Leaf explants of Echinacea purpurea L. taken from aseptically germinated
seedlings were inoculated with A. tumefaciens strains EHA105, carrying a binary
vector conferring herbicide resistant bar gene and fungal resistant chitinase gene.
Glufosinate ammonium‐resistant shoots were regenerated on a medium
containing BAP and NAA at a concentration of 4.88 and 0.053 μM, respectively.
A subsequent transfer of shoots to medium containing BAP was necessary for
stem elongation and leaf development.
The purpose of this book is to provide the advances in plant in vitro culture as related to perennial fruit crops and medicinal plants. Basic principles and new techniques, now available, are presented in detail. The book will be of use to researchers, teachers in biotechnology and for individuals interested to the commercial application of plant in vitro culture.