Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Distinguishing between linear and exponential cell growth during the division cycle: Single-cell studies, cell-culture studies, and the object of cell-cycle research
Technical advancements are basic elements in our life. In biophysical studies, new applications and improvements in well-established techniques are being implemented every day. This book deals with advancements produced not only from a technical point of view, but also from new approaches that are being taken in the study of biophysical samples, such as nanotechniques or single-cell measurements.
Cell biology studies the structural and physiological properties of cells, including their behaviors, interactions, and environment. This is done on both the microscopic and molecular levels, for single-celled organisms such as bacteria as well as the specialized cells in multicellular organisms such as humans. Understanding the structure and function of cells is fundamental to all of the biological sciences. The similarities and differences between cell types are particularly relevant to molecular biology....
Several general principles have arisen from these studies. Bevacizumab appears to potentiate the effects of many different types of active chemotherapeutic regimens used to treat a variety of different tumor types. No phase III trials have demonstrated single-agent activity for bevacizumab; colon and lung cancer trials have demonstrated a lack of activity when used alone. An exception may be renal cell cancer (RCC), a tumor that is specifically dependent upon VEGF as the result of deletion of the VHL tumor suppressor and activation of the HIF-1α transcription factor (see above).
Here, results are produced only when an exceptional
condition is reached (the temperature is outside the desired
range), and thus relatively few tuples will match. We note
that this is a low selectivity query, indicating that it outputs
(selects) a small percentage of the original sensor tuples.
As mentioned above, our discussions with engineers in
industrial settings suggest that each sensor may have sev-
eral alarm conditions associated with it, and there may be
hundreds or thousands of sensors in a single factory.
Diaminopelargonic acid aminotransferase (DAPA AT), which is involved
in biotin biosynthesis, catalyzes the transamination of 8-amino-7-oxonona-noic acid (KAPA) using S-adenosyl-l-methionine (AdoMet) as amino
donor.Mycobacterium tuberculosisDAPA AT, a potential therapeutic tar-get, has been overproduced inEscherichia coliand purified to homogeneity
using a single efficient step on a nickel-affinity column.
TheB class cell-attachedephrinsmediate contact-dependent
cell–cell communications and transduce the contact signals
to the host cells through the binding interactions of their
cytoplasmic domains. Two classes of intracellular effectors
of B ephrins have been identified: one contains the PSD-95/
Dlg/ZO-1 (PDZ)domain(for examplePDZ-RGS3), and the
second the Src homology 2 (SH2) domain (e.g. the Grb4
In humans, thromboxane (TX) A2 signals through two receptor isoforms,
thromboxane receptor (TP)aand TPb, which are transcriptionally regula-ted by distinct promoters, Prm1 and Prm3, respectively, within the single
TP gene. The aim of the current study was to investigate the ability of the
endogenous peroxisome proliferator-activated receptor (PPAR)c ligand
-prostaglandin J2 (15d-PGJ2) to regulate expression of the
human TP gene and to ascertain its potential effects on the individual TPa
Thermobifida fuscaexocellulase Cel6B acts by an inverting hydrolysis mech-anism; however, the catalytic acid and base residues for this enzyme have
not been confirmed. Site-directed mutagenesis and kinetic studies were used
to show that Asp274 is the catalytic acid, which is consistent with what is
found for other members of family-6 glycoside hydrolases; however, a sin-gle catalytic base was not identified.
Campylobacter jejunihosts two hemoglobins (Hbs). TheCamplylobacter je-juni single-domain Hb (called Cgb) is homologous to the globin domain of
flavohemoglobin, and it has been proposed to protect the bacterium against
A catalytic turnover of supercoiled DNA (scDNA) transformation medi-ated by topoisomerases leads to changes in the linking number (Lk) of the
polymeric substrate by 1 or 2 per cycle. As a substrate of the topoisomeri-zation reaction it is chemically identical to its product; even a single
catalytic event results in the quantum leap in the scDNA topology.
Exoribonuclease II (RNase II), encoded by thernb gene, is a ubiquitous
enzyme that is responsible for 90% of the hydrolytic activity in
Escherichia colicrude extracts. The E. colistrain SK4803, carrying the
mutant allelernb296, has been widely used in the study of the role of
RNase II. We determined the DNA sequence ofrnb296 and cloned this
mutant gene in an expression vector. Only a point mutation in the coding
sequence of the gene was detected, which results in the single substitution
of aspartate 209 for asparagine....
This study describes the construction of a library of single-chain antibody
fragments (scFvs) from a single human donor by individual amplification
of all heavy and light variable domains (1.1·10
recombinants). The lib-rary was panned using the phage display technique, which allowed selection
of specific scFvs (3F and C1) capable of recognizing Cn2, the major toxic
component of Centruroides noxiusscorpion venom.
The catalytic activity of phenylalanine hydroxylase (PAH,
phenylalanine 4-monooxygenase EC 18.104.22.168) is regulated
by three main mechanisms, i.e. substrate (L-phenylalanine,
L-Phe) activation, pterin cofactor inhibition and phos-phorylation of a single serine (Ser16) residue.To address the
molecular basis for the inhibition by the natural cofactor
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin, its effects on the
recombinant tetrameric human enzyme (wt-hPAH) was
studied using three different conformational probes, i.e.
The primary purpose of the present study was to investi-gate whether DNA replication at meiotic prophase also
requires replication factors, especially proliferating cell
nuclear antigen (PCNA). We cloned PCNA cDNAs
(CoPCNA) from a cDNA library made from basidia of the
basidiomycete,Coprinus cinereus. Interestingly, although
CoPCNAis a single-copy gene in the genome, two dier-entPCNAcDNA species were isolated using degenerate
primers and a meiotic cDNA library, and were designated
Recently, we have identiﬁed and puriﬁed minisatellite DNA binding proteins (MNBPs) that bind to the mouse hypervariable minisatellite Pc-1, from NIH3T3 cells. This study describes the isolation and characterization of a mouse leucine-rich protein (mLRP130) as one of the MNBPs that binds to the C-rich strand of Pc-1. The mLRP130 cDNA was demonstrated to encode a polypeptide of 1306 aminoacid residues with a deduced molecular mass of 137 kDa, and the mLRP130 mRNA is detected in various organs, including heart, brain, liver, skeletal muscle, kidneys and testes.
DRAM Cell ObservationDRAM Cell Observations.1T DRAM requires a sense amplifier for each bit line, due
to charge redistribution read-out.DRAM memory cells are single ended in contrast to
SRAM cells.The read-out of the 1T DRAM cell is destructive; read
and refresh operations are necessary for correct
Although in many trials haloperidol has been used as
the standard comparator, the clinical trial evidence for haloperidol itself is much less
impressive than one might expect (Barry et al 2012). By their very nature systematic reviews
and RCTs provide only average indices of probable efficacy in groups of individuals recruited
to the study in question. Although many RCTs attempt to limit inclusion criteria to a single
category of diagnosis from DSM4 or ICD10, many studies include individuals with different
types of schizophrenic diagnosis such as schizoaffective disorder.
PCR detection of viral pathogens is extremely useful, but suffers from the
challenge of detecting the many variant strains of a given virus that arise
over time. Here, we report the computational derivation and initial experi-mental testing of a combination of 10 PCR primers to be used in a single
high-sensitivity mixed PCR reaction for the detection of dengue virus.