Polarized membrane sorting of connexin 43 (Cx43) has not been well-char-acterized. Based on the presence of a putative sorting signal, YKLV(286–
289), within its C-terminal cytoplasmic domain, we hypothesized that Cx43
is selectively expressed on the basolateral surface of Madin–Darby canine
kidney (MDCK) cells in a tyrosine-dependent manner.
Topogenesis of cytochrome P450scc, a resident proteinof the
inner membrane of adrenocortical mitochondria, is still
obscure. In particular, little is known about the cause of its
tissue specificity. In an attempt to clarify this point, we
examined the process in Saccharomyces cerevisiae cells
synthesizing cytochrome P450scc as its native precursor
(pCYP11A1) or versions inwhich its N-terminal addressing
presequence had been replaced with those of yeast mitoch-ondrial proteins: CoxIV(1–25) and Su9(1–112)....
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells
Not long ago, social media seemed so new and different that it was treated as an appendage of
sorts—a kind of marketing that should be tried only by “experts.”
While that view still exists to some degree today, it’s become clear to many that social media is no
longer marketing’s new thing. It’s now simply part of the way we do marketing today.
I believe that the proper way to view social media from a small-business owner’s point of view is
as more of an evolution than a revolution.
The level of intracellular proteins is mainly regulated through modifications
by ubiquitin ligases that target them for degradation. Members of the
NEDD4 family of E3 ubiquitin ligases, such as Itch (atrophin-1 interacting
protein 4), possess up to four WW domains for specific association with
PY motif-containing substrates.
We have observed that the more that is captured, the more correlations are possible to help find things. For
example, suppose you want to refer to a document you recall viewing when visiting Boston last year. A
GPS trail or travel calendar entry can be used a the starting point for a search performed for all events from
the same day, where the entry for viewing the document along with its name and thumbnail appears. We
could multiply examples of this sort – perhaps you recall it was a hot day; perhaps you remember an
Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells
In receptor-mediated sorting of soluble protein ligands in the endomem-brane system of eukaryotic cells, three completely different receptor pro-teins for mammalian (mannose 6-phosphate receptor), yeast (Vps10p) and
plant cells (vacuolar sorting receptor; VSR) have in common the features
of pH-dependent ligand binding and receptor recycling.
Phage display of antibody libraries is a powerful tool for antibody discov-ery and evolution. Recombinant antibodies have been displayed on phage
particles as scFvs or Fabs, and more recently as bivalent F(ab¢)2.We
recently developed a technology (E-clonal) for screening of combinatorial
IgG libraries using bacterial periplasmic display and selection by fluores-cence-activated cell sorting (FACS) [Mazor Yet al.
Gangliosides are glycosphingolipids mainly present at the outer leaflet of
the plasma membrane of eukaryotic cells, where they participate in recogni-tion and signalling activities. The synthesis of gangliosides is carried out in
the lumen of the Golgi apparatus by a complex system of glycosyltrans-ferases.
Correct sorting of newly synthesized peroxisomal matrix
proteins is dependent on a peroxisomal targeting signal
(PTS).So far two PTSs are known.PTS1 consists of a
tripeptide that is located at the extremeCterminus ofmatrix
proteins and is specifically recognized by the PTS1-receptor
Pex5p.We studied Hansenula polymorpha Pex5p
(HpPex5p) using fluorescence spectroscopy.The intensity of
Trp fluorescence of purified HpPex5p increased by 25%
upon shifting the pH frompH 6.0 to pH 7.2.
Subunits (a, b and c) of the interleukin-2 receptor complex (IL-2R) are involved in both proliferative and activationinduced cell death (AICD) signaling of T cells. In addition, the signaling b and c chains are shared by other cytokines (e.g. IL-7, IL-9, IL-15). However, the molecular mechanisms responsible for recruiting/sorting the a chains to the signaling chains at the cell surface are not clear.
And so, for another six years at least, it is over. Thousands of
authors referring to vast numbers of papers have, in sometimes-
contested consultation with the governments that lend
their name to the process, provided the world with their best assessment
to date of humanity’s prospects and options in the matter of
The Intergovernmental Panel on Climate Change (IPCC) is far
from a perfect institution, but it is a necessary and a heartening one.
Two banks were established in Scotland by charter from the king; one
the Bank of Scotland, in 1695; the other, the Royal Bank of Scotland, in
1727. These two banks have branches in most of the principal towns of
Scotland; but as they never obtained any exclusive privileges, a multi-
tude of private banks sprung up to dispute the business with them, and
to divide its profits.
This free competition among the banks, produced a new sort of
bank loans, which has given celebrity to the Scotch system of banking.
Calpain 7, a mammalian ortholog of yeast Cpl1⁄Rim13 and fungal PalB, is
an atypical calpain that lacks a penta-EF-hand domain. Previously, we
reported that a region containing a tandem repeat of microtubule-interact-ing and transport (MIT) domains in calpain 7 interacts with a subset of
endosomal sorting complex required for transport (ESCRT)-III-related
proteins, suggesting involvement of calpain 7 in the ESCRT system.
Protein sorting and assembly in membrane biogenesis and function involves
the creation of ordered domains of lipids known as membrane rafts. The
rafts are comprised of all the major classes of lipids, including glycero-phospholipids, sphingolipids and sterol. Cholesterol is known to interact
with sphingomyelin to form a liquid-ordered bilayer phase.
PA-TM-RING proteins have an N-terminal protease-associated domain, a
structure found in numerous proteases and implicated in protein binding,
and C-terminal RING finger and PEST domains. Homologous proteins
include GRAIL (gene related to anergy in leukocytes), which controls
T-cell anergy, and AtRMR1 (receptor homology region-transmembrane
domain-RING-H2 motif protein), a plant protein storage vacuole sorting
Despite the critical importance of protein ubiquitination in the regulation
of diverse cellular processes, the molecular mechanisms by which cells rec-ognize and transmit ubiquitin signals remain poorly understood. The
endosomal sorting machinery component hepatocyte growth factor-regu-lated tyrosine kinase substrate (Hrs) contains a ubiquitin-interacting motif
(UIM), which is believed to bind ubiquitinated membrane cargo proteins
and mediate their sorting to the lysosomal degradation pathway.
In order to evaluate the ability of the cell-penetrating
a-helical amphipathic model peptide KLALKLALKALK
AALKLA-NH2 (MAP) to deliver peptide nucleic acids
(PNAs) into mammalian cells, MAP was covalently linked
to the 12-mer PNA 5¢-GGAGCAGGAAAG-3¢ directed
against themRNAof the nociceptin/orphaninFQ receptor.
The cellular uptake of both the naked PNA and its MAP-conjugate was studied by means of capillary electrophoresis
combined with laser-induced fluorescence detection, confo-cal laser scanningmicroscopyandfluorescence-activatedcell