Specimen preparation

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  • Transmission electron microscopy (TEM) is a technique where the electron-beam is transmitted through an ultra-thin specimen, interacting with specimen as it passes through it. An image is formed from the interaction of the electrons transmitted through the specimen, which is then magnified and focused onto an imaging device, such as a fluorescent screen, a photographic film, or a charge-coupled device (CCD) sensor. This technique is capable of imaging at significantly high resolution than the light microscopes, owing to the small de-Broglie wavelength of electrons....

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  • Tham khảo sách 'the transmission electron microscope_1', kỹ thuật - công nghệ, hoá học - dầu khí phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả

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  • This book is designed to explain to dental students the processes of diagnosis and treatment planning, through consideration of clinical cases and problems associated with aspects of all dental specialties. It presents a series of case histories from all the major areas of dentistry, and uses a question-and-answer format to guide readers through the process of examination, differential diagnosis, investigations, diagnosis and treatment. It prepares readers for the wide variety of problems likely to be encountered in clinical practice.

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  • The information nurses need when, where, and how they need it! Here s the information nurses and nursing students need to know about laboratory and diagnostic tests! This nursing-focused, easy-to-read handbook provides all of the information you need to understand how tests work, interpret their results, and provide quality patient care pre-test, intra-test, and post-test. Tests and procedures are listed in alphabetical order by their complete name for quick reference.

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  • Introduction: Pentazocine ( Fig. 5.1) is a non-narcotic analgesic, but shows mild dependence; there are many cases of pentazocine abuse especially for medical and co-medical workers. The drug is being regulated as a subclass compound of narcotics (DEA class: IV). Pentazocine in both antemortem and postmortem specimens is being analyzed by GC [1–4], HPLC [5–7] and GC/MS [8]. In this chapter, a simple method for GC/MS analysis of pentazocine in human whole blood and urine is presented. ⊡ Figure 5.1 Structures of pentazocine and dextromethorphan (IS).

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  • Molecular Cytogenetics The introduction of FISH methodologies in the late 1980s revolutionized the field of cytogenetics. In principle, FISH is similar to other DNA-DNA hybridization methodologies. The probe is labeled with a hapten, such as biotin or digoxigenin, to allow detection with a fluorophore (e.g., FITC or rhodamine). After the hybridization step, the specimen is counter-stained and the preparations are visualized with a fluorescence microscope.

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  • Sputum samples were fi rst classifi ed according to their macroscopic aspect in the laboratory. Salivary samples were immediately removed and a new sample was requested. The time between sputum collection and analysis was recorded. A 20–30 mm smear was made on a new slide with a wooden applicator. After heat fi xation, hot Ziehl-Neelsen (ZN) staining was performed: carbol fuchsin 0.3%, slow heating until steaming, rinsing after 5 min, destaining with 25% sulfuric acid, rinsing, counterstaining with methylene blue 0.3% for 1 min, rinsing and drying.

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  • Figure 79-8 A cDNA array experiment. RNA is prepared from cells, reverse transcribed to cDNA, and labeled with fluorescent dyes (typically green for normal cells and red for cancer cells). The fluorescent probes are mixed and hybridized to the cDNA array. Each spot on the array is a cDNA fragment that represents a different gene. The image is then captured with a fluorescence camera; red spots indicate higher expression in tumor compared with reference while green spots represent the opposite. Yellow signals indicate equal expression levels in normal and tumor specimens.

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  • A series of cobalt doped nickel ferrite with composition of Ni1-XCoXFe2O4 with x ranges from 0.0 to 0.8 (in steps of 0.2) was prepared by using co-precipitation method and subsequently sintered, annealed at 6000C for 3h. The influence of the Co content on the crystal lattice parameter, the stretching vibration and the magnetization of specimens were subsequently studied. XRD and FTIR were used to investigate structure and composition variations of the samples. All samples were found to have a cubic spinel structure. ...

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  • During the past decade or so, a wealth of information about metabolites in various human brain tumour preparations (cultured cells, tissue specimens, tumours in vivo) has been accumulated by global profiling tools. Such hol-istic approaches to cellular biochemistry have been termed metabolomics.

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  • This paper present the results of research on designing mold for vacuum infusion technique for preparation the polymer composite material. Specimens of varrious methods of vacuum technique have been prepared and their mechanical and structural properties have been studied. The results show that material obtained from vacuum assisted technique have a preciously mechanical properties, increased in average 45% in comparison with hand lay up method. The scanning electronic microscopy has been used for investigation on void appearance of composite materials.

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