Strand separation

Xem 1-10 trên 10 kết quả Strand separation
  • There is still a controversy over the mechanism of promoter DNA strand separation upon open transcription complex (RPo) formation by Escheri-chia coli RNA polymerase: is it a single or a stepwise process controlled by Mg 2+ ions and temperature? To resolve this question, the kinetics of pseudo-first-order oxidation of thymine residues by KMnO4 in the )11…+2 DNA region of RPo at thekPRpromoter was examined under single-hit conditions as a function of temperature (13–37C) in the absence or presence of 10 mmMgCl2....

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  • Concept of hybrid molecules: When double strand DNA is steamed to a temperature exceed the melting temperature (Tm), it will separate into 2 single strands DNA due to breaks of H bonds. If the reaction temperature is then decreased slowly plus other appropriate experimental conditions, these ssDNA will pair again. This phenomenon is called the hybridization of molecules. Characteristic of the hybrid molecules: Specificity, the re-pairing occurs only between two complementary sequences.

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  • The term DNA sequencing refers to methods for determining the order of the nucleotides bases adenine,guanine,cytosine and thymine in a molecule of DNA. The first DNA sequence were obtained by academic researchers,using laboratories methods based on 2- dimensional chromatography in the early 1970s. By the development of dye based sequencing method with automated analysis,DNA sequencing has become easier and faster.

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  • Automated DNA sequencing is a core research tool used by almost every research biochemistry lab. It is used to determine the sequence of DNA, or the genetic code, that serves as the blueprint of life for every organism on Earth.

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  • The vocabulary component of the course is designed to develop students' awareness and ability across a wide range of vocabulary skills. The first section focuses on effective dictionary skills, as a key foundation for further vocabulary development. Subsequent sections feature a variety of topic vocabulary and idioms, and there are also three separate strands which run throughout the book: word formation (including word families, collocation and phrasal verbs).

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  • When mounting to a pole is not possible due to a lack of space, strand mounting is the common alternative. During an installation, the MST universal bracket is easily strand mounted using standard materials already available to the installer. A separate strand mounting bracket can sell for as much as $15 and, again, may not always be readily available or even the proper one for a particular deployment.

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  • More discrete sequence alterations rely heavily on the use of the PCR, which allows rapid gene amplification and analysis. Moreover, PCR makes it possible to perform genetic testing and mutational analysis with small amounts of DNA extracted from leukocytes or even from single cells, buccal cells, or hair roots. Screening for point mutations can be performed by numerous methods (Table 62-9); most are based on the recognition of mismatches between nucleic acid duplexes, electrophoretic separation of single- or double-stranded DNA, or sequencing of DNA fragments amplified by PCR.

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  • This book emerges from two separate, but intersecting, strands of work that began in the late 1980s, when the World Bank ini- tiated a review of priorities for the control of specific diseases. The review generated findings about the comparative cost- effectiveness of interventions for most diseases important in developing countries. The purpose of the cost-effectiveness analysis (CEA) was to inform decision making within the health sectors of highly resource-constrained countries.

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  • The occurrence of DNA double-strand breaks in the nucleus provokes in its structural organization a large-scale alteration whose molecular basis is still mostly unclear. Here, we show that double-strand breaks trigger pref-erential assembly of nucleoproteins in human cellular fractions and that they mediate the separation of large protein–DNA aggregates from aque-ous solution.

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  • DNA helicases are molecularmotor enzymes that use the energy of NTP hydrolysis to separate transiently energetic-ally stable duplex DNA into single strands. They are there-fore essential in nearly all DNA metabolic transactions. They act as essential molecular tools for the cellular machinery. Since the discovery of the first DNA helicase in Escherichia coliin1976, several havebeen isolated fromboth prokaryotic and eukaryotic systems.

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