Synthetic gene

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  • Computational synthetic biology has borrowed methods, concepts, and techniques from systems biology and electrical engineering. Features of tools for the analysis of biochemical networks and the design of electric circuits have been combined to develop new software, where Standard Biological Parts (physically stored at the MIT Registry) have a mathematical description, based on mass action or Hill kinetics, and can be assembled into genetic networks in a visual, “drag & drop” fashion.

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  • Yellow stem borer has been identified as a major insect pest of deepwater rice, causing severe yield losses. Bt gene(s) from Bacillus thuringiensis have been proven very effective in pest resistance program. The use of transgenic plants expressing Bt gene(s) is now occupied effective approach to control insect infestation. We have successfully introduced a synthetic cryIA(b) gene into embryogenic calli of a deepwater indica rice variety, Vaidehi, by using the biolistic method of transformation.

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  • Trong vài năm gần đây đã xuất hiện một diễn tiến khoa học tuy còn trong giai đoạn thầm lặng, phôi thai, nhưng đang hứa hẹn mang lại một cuộc cách mạng mới trong sinh học bởi tiềm năng ứng dụng lớn lao trong xã hội, gồm việc chế tạo dược phẩm, tạo năng lượng và xử lý môi trường. Ðó là sự ra đời của bộ môn sinh học tổng hợp(synthetic biology) hay còn gọi là sinh học kiến tạo (constructive biology), dựa trên kỹ thuật tổng hợp và điều hòa gene. ...

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  • The explosion of new technologies in the post-genomic era is allowing the study of biological systems on a genome-wide scale, at the main functional genomic levels: (epi)-genome, transcriptome, proteome, endo- and exometabolome, and their interactions. At the same time that these techniques are being applied and refined, the complexity of biological systems is being rediscovered (1) with thousands of components (e.g., genes, transcripts, proteins, metabolites) participating in finely tuned metabolic and regulatory networks.

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  • Genetics is the science of genes, heredity, and the variation of organisms.[37][38] Genes encode the information necessary for synthesizing proteins, which in turn play a large role in influencing (though, in many instances, not completely determining) the final phenotype of the organism. In modern research, genetics provides important tools in the investigation of the function of a particular gene, or the analysis of genetic interactions. Within organisms, genetic information generally is carried in chromosomes, where it is represented in the chemical structure of particular DNA molecules....

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  • Synthetic polymers fulfill many functions in biotechnology and medicine. In cell culture technology and tissue engineering they provide the surfaces to which cells may attach. Cross-linked polymer networks are used for drug delivery and cell encapsulation. Polymer-based porous membranes can be used to shield implanted cells from the immune system of the host, while allowing for the exchange of nutrients and metabolic waste products thus keeping the cells alive and functioning.

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  • Game theory provides a powerful mathematical framework that can accommodate the preferences and requirements of various stakeholders in a given process as regards the outcome of the process. The chapters' contents in this book will give an impetus to the application of game theory to the modeling and analysis of modern communication, biology engineering, transportation, etc...

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  • A novel vector platform for vitamin H-inducible transgene expression in mammalian cells have therefore designed a strategy to convert antibiotic-responsive transcription factors into gene regulation systems responsive to non-toxic biotin, also known as vitamin H.

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  • Liver fibrosis ranks as the second cause of death in México's productive-age population. This pathology is characterized by acummulation of fibrillar proteins in hepatic parenchyma causing synthetic and metabolic disfunction. Remotion of excessive fibrous proteins might result in benefit for subjects increasing survival index. The goal of this work was to find whether the already known therapeutical effect of human urokinase Plasminogen Activator and human Matrix Metalloprotease 8 extends survival index in cirrhotic animals.

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  • Modern biotechnology can be used to manufacture existing medicines relatively easily and cheaply. The first genetically engineered products were medicines designed to treat human diseases. To cite one example, in 1978 Genentech developed synthetic humanized insulin by joining its gene with a plasmid vector inserted into the bacterium Escherichia coli. Insulin, widely used for the treatment of diabetes, was previously extracted from the pancreas of abattoir animals (cattle and/or pigs).

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  • important regulators of biological processes in animals and plants. MiRNAs regulate gene expression at the posttranscriptional level by binding to mRNAs and either inhibit translation or modify the stability of the mRNA. Due to the important biological role of miRNAs it is of great interest to study their expression level in the cells. Furthermore, miRNAs have been associated with cancer and other diseases [1] and miRNA expression can help in the diagnosis and prognostic of human disease [2,3].

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  • ITuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: nhibition of core gene of HCV 3a genotype using synthetic and vector derived siRNAs

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  • The regulation of cytokines and pro-inflammatory genes is an absolute essentiality to combat inflammatory diseases. The present study investigated the effects of 4-O-chloroacetyl-2,3-di-O-acetyl-6-O-levulinoyl-b-d-glucopyr-anosyl]-(1-3)-1-O-(p-methoxyphenyl)-2-deoxy-2-N-trichloroacetyl-4,6-O-ben-zylidene-a-d-glucopyranoside (RSCL-0409), a novel small molecule Toll-like receptor (TLR) signalling antagonist, and its mechanism of action in human monocytic (THP-1) cells stimulated with lipopolysaccharide (LPS)....

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  • ThepyrGgene fromLactococcus lactisencodes CTP syn-thase (EC 6.4.3.2), an enzyme converting UTP to CTP. A series of strains were constructed with different levels of pyrGexpression by insertion of synthetic constitutive pro-moters with different strengths in front ofpyrG.These strains expressed pyrGlevels in a range from 3 to 665% relative to thewild-type expression level.Decreasing the level of CTP synthase to 43% had no effect on the growth rate, showing that the capacity of CTP synthase in the cell is in excess in a wild-type strain. ...

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  • Wilson disease (WD), an inherited disorder affecting copper metabolism, is characterized by hepatic cirrhosis and neuronal degeneration, which result from toxic levels of copper that accumulate in the liver and brain, respectively. We reported previously that the  1.3-kb promoter of the WD gene contains four metal response elements (MREs). Among the four MREs, MREa plays the most important role in the transcriptional activation of the WD promoter.

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  • Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Synthetic lethal analysis of Caenorhabditis elegans posterior embryonic patterning genes identifies conserved genetic interactions...

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  • The Springer Handbook of Auditory Research presents a series of comprehensive and synthetic reviews of the fundamental topics in modern auditory research. The volumes are aimed at all individuals with interests in hearing research, including advanced graduate students, postdoctoral researchers, and clinical investigators. The volumes are intended to introduce new investigators to important aspects of hearing science and to help established investigators to better understand the fundamental theories and data in fields of hearing that they may not normally follow closely....

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  • The abundance of the doubly substituted CO2 isotopologue, 13C18O16O, in CO2 produced by phosphoric acid digestion of synthetic, inorganic calcite and natural, biogenic aragonite is proportional to the concentration of 13C–18O bonds in reactant carbonate, and the concentration of these bonds is a function of the temperature of carbonate growth. This proportionality can be described between 1 and 50 C by the function: D47 = 0.0592 Æ 106 Æ T2  0.

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  • Cell-free protein synthesis offers rapidaccess toproteins that are selectively labelledwith [ 15 N]aminoacids andsuitable for analysis by NMR spectroscopy without chromatographic purification. A system based on anEscherichia colicell ex-tractwas optimizedwith regard toproteinyieldandminimal usage of 15 N-labelled amino acid, and examined for the presence of metabolic by-products which could interfere with the NMR analysis. Yields of up to 1.8 mg of human cyclophilin A per mL of reactionmedium were obtained by expression of a synthetic gene. ...

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  • Two isoforms of a heme oxygenase gene,ho1andho2, with 51% identity in amino acid sequence have been identified in the cyanobacterium Synechocystissp. PCC 6803. Isoform-1, Syn HO-1, has been characterized, while isoform-2, Syn HO-2, has not. In this study, a full-lengthho2gene was cloned using synthetic DNA and Syn HO-2 was demonstrated to be highly expressed inEscherichia colias a soluble, catalytically active protein.

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