Porcine heart cytoplasmic malate dehydrogenase (s-MDH) is a dimeric protein (2 · 35 kDa). We have studied equilibrium unfolding and refolding of s-MDH using activity assay, ﬂuorescence, far-UV and near-UV circular dichroism (CD) spectroscopy, hydrophobic probe-1-anilino8-napthalene sulfonic acid binding, dynamic light scattering, and chromatographic (HPLC) techniques. The unfolding and refolding transitions are reversible and show the presence of two equilibrium intermediate states.
Ju Park et al. Nanoscale Research Letters 2011, 6:223 http://www.nanoscalereslett.com/content/6/1/223
Optimal synthesis and characterization of Ag nanofluids by electrical explosion of wires in liquids
Eun Ju Park1, Seung Won Lee2, In Cheol Bang2, Hyung Wook Park1*
Abstract Silver nanoparticles were produced by electrical explosion of wires in liquids with no additive. In this study, we optimized the fabrication method and examined the effects of manufacturing process parameters.
General Compositional Layout Information
A good basic composition will often either be asymetrical or will lead the viewer's eye around the work. Some standard compositional layouts are shown above.
Quadrant-In this simple composition a dynamic balance is created by
the changes in value from dark to light.
Clearly, man-made projects are not new: monuments surviving from the earliest civilizations testify
to the incredible achievements of our forebears and still evoke our wonder and admiration. Modern
projects, for all their technological sophistication, are not necessarily greater in scale than some
of those early mammoth works.