Glutathione (GSH) conjugating enzymes, glutathioneS-transferases (GSTs),
are present in different subcellular compartments including cytosol,
mitochondria, endoplasmic reticulum, nucleus and plasma membrane. The
regulation and function of GSTs have implications in cell growth, oxidative
stress as well as disease progression and prevention.
Bài giảng "Công nghệ di truyền - Chương 1: Các enzyme chủ yếu" cung cấp cho người học các kiến thức: Enzyme cắt giới hạn, Methyl hóa, Enzyme terminal transferase, phản ứng cắt bằng RE, DNA polymerase, DNA polymerase ở Prokaryote,... Mời các bạn cùng tham khảo nội dung chi tiết.
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients...
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Genetic polymorphisms in glutathione S-transferase (GST) superfamily and risk of arsenic-induced urothelial carcinoma in residents of southwestern Taiwan
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The metal tolerance profile of Thlaspi goesingense is mimicked in Arabidopsis thaliana heterologously expressing serine acetyl-transferase
Mouse liver glutathione transferase P1-1 has three cysteine residues at posi-tions 14, 47 and 169. We have constructed the single, double and triple cys-teine to alanine mutants to define the behaviour of all three thiols. We
confirm that C47 is the ‘fast’ thiol (pK7.4), and define C169 as the alkaline
reactive residue with a pKa
Bài giảng "Chương 1: Các Enzyme chủ yếu" cung cấp cho người học các kiến thức: Enzyme cắt giới hạn, Enzyme terminal transferase, phản ứng cắt bằng RE, DNA polymerase ở Prokaryote, DNA polymerase bền nhiệt, DNA polymerase bền nhiệt có hoạt tính sửa sai,... Mời các bạn cùng tham khảo.
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Anticitrullinated protein antibody (ACPA) in rheumatoid arthritis: influence of an interaction between HLA-DRB1 shared epitope and a deletion polymorphism in glutathione s-transferase in a cross-sectional study...
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Oral ezatiostat HCl (Telintra(R), TLK199) and Idiopathic Chronic Neutropenia (ICN): A case report of complete response of a patient with G-CSF resistant ICN following teatment with ezatiostat, a glutathione S-transferase P1-1 (GSTP1-1) inhibitor...
GlutathioneS-transferases (GSTs) catalyze the conjugation of glutathione
to hydrophobic compounds, contributing to the metabolism of toxic
chemicals. In this study, we show that two naturally occurring tau GSTs
(GSTUs) exhibit distinctive kinetic parameters towards 1-chloro-2,4-dini-trobenzene (CDNB), although they differ only in three amino acids
(Arg89, Glu117 and Ile172 in GSTU1 are replaced by Pro89, Lys117 and
Val172 in GSTU2).
A family 16 glycoside hydrolase, xyloglucan xyloglucosyl transferase
(EC 184.108.40.206), also known as xyloglucan endotransglycosylase (XET), and
designated isoenzyme HvXET6, was purified approximately 400-fold from
extracts of young barley seedlings.
Onchocerciasis or river blindness, caused by the filarial nematodeOncho-cerca volvulus, is the second leading cause of blindness due to infectious
diseases. The protective role of the omega-class glutathione transferase 3
from O. volvulus(OvGST3) against intracellular and environmental reactive
oxygen species has been described previously.
A highly active glutathioneS-transferase was purified from adult German
cockroaches,Blattella germanica. The purified enzyme appeared as a single
band of 24 kDa by SDS⁄PAGE, and had a different electrophoretic mobil-ity than, a previously identified Sigma class glutathione S-transferase
(Bla g 5).
S-(2,3-Dichlorotriazinyl)glutathione (SDTG) was synthes-ized and shown to be an effective alkylating affinity label
for recombinant maize glutathioneS-transferase I (GST I).
InactivationofGST IbySDTGatpH 6.5 followedbiphasic
pseudo-first-order saturation kinetics. The biphasic kinetics
can be described in terms of a fast initial phase of inactiva-tion followed by a slower phase, leading to 42 ± 3%
4-Hydroxy-2-trans-nonenal (4-HNE), one of the major end
products of lipid peroxidation, has been shown to induce
apoptosis in a variety of cell lines. It appears to modulate
signalingprocesses inmore thanonewaybecause it has been
suggested to have a role in signaling for differentiation and
proliferation. We showfor the first time that incorporation
of 4-HNE-metabolizing glutathioneS-transferase (GST)
isozyme, hGSTA4-4, into adherent cell lines HLE B-3 and
CCL-75, by either cDNA transfection or microinjection of
active enzyme, leads to their transformation. ...
UDP-N-acetylglucosamineenolpyruvyl transferase (MurA),
catalyzes the first step in the biosynthesis of peptidoglycan,
involving the transfer of the intactenolpyruvyl moiety from
phosphoenolpyruvate to the 3¢-hydroxyl group of UDP-N-acetylglucosamine (UDPNAG). The enzyme is irreversibly
inhibited by the antibiotic fosfomycin. The inactivation is
caused by alkylation of a highly conserved cysteine residue
(C115) that participates in the binding of phos-phoenolpyruvate.
Rubber transferase, acis-prenyltransferase, catalyzes the
addition of thousands of isopentenyl diphosphate (IPP)
molecules toanallylic diphosphate initiator, suchas farnesyl
diphosphate (FPP,1), in the presence of a divalent metal
cofactor. In an effort to characterize the catalytic site of
rubber transferase, the effects of two types of protein
farnesyltransferase inhibitors, several chaetomellic acid A
analogs (2,4–7)anda-hydroxyfarnesylphosphonic acid (3),
on the ability of rubber transferase to add IPP to the allylic
diphosphate initiator were determined. ...
Propionate CoA-transferase fromClostridium propionicum
hasbeenpuri®edand thegene encoding the enzymehasbeen
cloned and sequenced. The enzyme was rapidly and irre-versibly inactivated by sodium borohydride or hydroxyl-amine in the presence of propionyl-CoA. The reduction of
the thiol ester between a catalytic site glutamate and CoA
with borohydride and the cleavage by hydroxylamine were
used to introduce a site-speci®c label, which was followed
by MALDI-TOF-MS. This allowed the identi®cation of
glutamate 324 at the active site. ...
Two genes (CM-AAT1 and CM-AAT2) with strong sequence homology (87% identity at the protein level) putatively involved in the formation of aroma volatile esters have been isolated from Charentais melon fruit. They belong to a large and highly divergent family of multifunctional plant acyl-transferases and show at most 21% identity to the only other fruit acyl-transferase characterized so far in strawberry. RT-PCR studies indicated that both genes were speciﬁcally expressed in fruit at increasing rates in the early and mid phases of ripening.
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: 4-Hydroxynonenal induces apoptosis in human osteoarthritic chondrocytes: the protective role of glutathione-S-transferase...