Kỹ thuật xác định Tumor maker theo phương pháp này có thể tóm tắt như sau
1. Pha rắn 2. Kháng thể đơn dòng I.
3. Kháng nguyên (Tumor Marker).
4. Kháng thể II và chất phát tin (phóng xạ hay huỳnh quang hoặc enzym).
(1) Pha rắn (Steptavidin) - một lớp tráng gắn vào mặt trong thành ống nghiệm.
(2) Kháng thể đơn dòng I - gắn vào thành ống nghiệm.
For decades we have been learning about the interplay between tumors and the
immune system. Our knowledge seemed somewhat incomplete and indirect, like
listening to the ocean waves through a shell. Only recently, cancer immunotherapy has
started to become a reality, with Provenge (Dendreon Corporation, WA), an
autologous antigen-presenting cell preparation, earning the approval of United States
Food and Drug Administration (FDA) for the treatment of advanced prostate cancer in
Publishing Process Manager Iva Lipovic Technical Editor InTech DTP team Cover InTech Design team First published January, 2013 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from email@example.com Research Directions in Tumor Angiogenesis, Edited by Jianyuan Chai p. cm. ISBN 978-953-51-0963-1
The urgent need for computer-assisted detection of tumors and lesions in medical
images becomes clear when one considers the state of affairs in X-ray film mammography
for breast cancer screening. In the United States it is estimated that
there are currently more than 50 million women over the age of 40 at risk of contracting
Summarizing a decade of scientific advance and therapeutic innovation, Renal Tumor offers all physicians treating kidney cancer, as well as researchers, updated information concerning the epidemiology, biology, and treatment of renal cell carcinoma. Contributors to this book are from all over the world and are experts in their individual fields.
Angiogenesis is an extension process of the cardiovascular network within human body. It is usually triggered by the demand of oxygen and nutrients from the fast growing tissue and uncontrollably dividing cells, as seen during wound healing and tumor progression. This book focuses on tumor angiogenesis and includes 8 chapters written by highly experienced scholars from five different countries.
Tham khảo sách 'evolution of the molecular biology of brain tumors and the therapeutic implications edited by terry lichtor', y tế - sức khoẻ, y học thường thức phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả
Ebook Tumor immunology methods and protocols contents: atmps for cancer immunotherapy: A regulatory overview 1 maria cristina galli, natural antibodies to Tumor-Associated antigens, generation and cryopreservation of clinical grade Wilms’ tumor 1
mRNA-Loaded dendritic cell vaccines for cancer immunotherapy,...
Gastroenteropancreatic neuroendocrine tumors (GEP-NETs) are rare and heterogeneous
neoplasms with overall increasing incidence, but not an associated increase in survival rate
over the past few decades. Tumors originate from at least 16 different cells of diffuse
endocrine system (DES), scattered through mucosa of gastrointestinal tract. They are mainly
sporadic, but sometimes exhibit familial inheritance.
Many tumor types exhibit an impaired Pasteur effect, i.e. despite the pres-ence of oxygen, glucose is consumed at an extraordinarily high rate com-pared with the tissue from which they originate – the so-called ‘Warburg
Extensive research has been performed to unravel the mechanistic signaling
pathways mediated by tumor necrosis factor receptor 1 (TNFR1), by con-trast there is limited knowledge on cellular signaling upon activation of
a-enolase (ENOA) is a metabolic enzyme involved in the synthesis of pyru-vate. It also acts as a plasminogen receptor and thus mediates activation of
plasmin and extracellular matrix degradation. In tumor cells, EMOAis
upregulated and supports anaerobic proliferation (Warburg effect), it is
expressed at the cell surface, where it promotes cancer invasion
DNA tumor viruses ensure genome amplification by hijacking the cellular
replication machinery and forcing infected cells to enter the S phase. The
retinoblastoma (Rb) protein controls the G1⁄S checkpoint, and is targeted
by several viral oncoproteins, among these the E7 protein from human
In early studies on energy metabolism of tumor cells, it was proposed that
the enhanced glycolysis was induced by a decreased oxidative phosphoryla-tion. Since then it has been indiscriminately applied to all types of tumor
cells that the ATP supply is mainly or only provided by glycolysis, without
an appropriate experimental evaluation.
Plasminogen activator inhibitor type 1 (PAI-1) is induced by many proin-flammatory and pro-oxidant factors. Among them, tumor necrosis factora
(TNFa), a pivotal early mediator that regulates and amplifies the develop-ment of inflammation, is one of the strongest PAI-1 synthesis activators.
Location of the TNFa response element in the PAI-1 promoter is still
trans-[PtCl2NH3(4-Hydroxymethylpyridine)] (trans-PtHMP) is an analogue
of clinically ineffective transplatin, which is cytotoxic in the human leuke-mia cancer cell line. As DNA is a major pharmacological target of anti-tumor platinum compounds, modifications of DNA by trans-PtHMP and
recognition of these modifications by active tumor suppressor protein p53
were studied in cell-free media using the methods of molecular biology and
Human chromosome 7 ORF 24 (C7orf24) has been identified as a tumor-related protein, and shown to be ac-glutamyl cyclotransferase. In the cur-rent study, we characterized the promoter region of the human C7orf24
gene to explore the transcriptional regulation of the gene.
The tumor suppressor p16
has functions beyond cell-cycle control via
cyclin-dependent kinases. A coordinated remodeling ofN- and O-glycosyla-tion, and an increase in the presentation of the endogenous lectin galectin-1 sensing these changes on the surface of p16
carcinoma cells (Capan-1), lead to potent pro-anoikis signals.