Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Differences in the way a mammalian cell and yeast cells coordinate cell growth and cell-cycle progression...
The glyoxylate cycle plays an essential role for anaplerosis of oxaloacetate
during growth of microorganisms on carbon sources such as acetate or
fatty acids and has been shown to contribute to virulence of several patho-gens.
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Research Article Computational Methods for Estimation of Cell Cycle Phase Distributions of Yeast Cells
This work concerns the cause of glycolytic oscillations in yeast. We analyse
experimental data as well as models in two distinct cases: the relaxation-like oscillations seen in yeast extracts, and the sinusoidal Hopf oscillations
seen in intact yeast cells. In the case of yeast extracts, we use flux-change
plots and model analyses to establish that the oscillations are driven by
on⁄off switching of phosphofructokinase.
The eukaryotic glyoxylate cycle has been previously hypothesized to occur in the peroxisomal compartment, which in the yeast Saccharomyces cerevisiae additionally represents the sole site for fatty acid b-oxidation. The subcellular location of the key glyoxylate-cycle enzyme malate synthase 1 (Mls1p), an SKL-terminated protein, was examined in yeast cells grown on dierent carbon sources.
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Modulation of the transcription regulatory program in yeast cells committed to sporulation...
A yeast model was generated to study the mechanisms and phenotypical
repercussions of expression of a-synuclein as well as the coexpression of
protein tau. The data show that aggregation ofa-synuclein is a nucleation–
elongation process initiated at the plasma membrane. Aggregation is con-sistently enhanced by dimethyl sulfoxide, which is known to increase the
level of phospholipids and membranes in yeast cells.
In both industrial fermenters and in their natural habitats, microorganisms
often experience an inhomogeneous and fluctuating environment. In this
paper we mimicked one aspect of this nonideal behaviour by imposing a
low and oscillating extracellular glucose concentration on nonoscillating
suspensions of yeast cells. The extracellular dynamics changed the intracel-lular dynamics – which was monitored through NADH fluorescence – from
steady to equally dynamic; the latter followed the extracellular dynamics at
the frequency of glucose pulsing....
The budding yeastSaccharomyces cerevisiaepossesses a very flexible and
complex programme of gene expression when exposed to a plethora of
environmental insults. Therefore, yeast cell homeostasis control is achieved
through a highly coordinated mechanism of transcription regulation invol-ving several factors, each performing specific functions. Here, we present
our current knowledge of the function of the yeast activator protein family,
formed by eight basic-leucine zipper trans-activators, which have been
shown to play an important role in stress response....
Engineered yeast cells efficiently convert ergosta-5-eneol to
pregnenolone and progesterone provided that endogenous
pregnenolone acetylase activity is disrupted and that
heterologous sterolD7-reductase, cytochrome P450 side
chain cleavage (CYP11A1) and 3b hydroxysteroid
dehydrogenase/isomerase (3b-HSD) activities are present.
CYP11A1activity requires the expressionof themammalian
NADPH-adrenodoxin reductase (Adrp) and adrenodoxin
(Adxp) proteins as electron carriers.
The objective of this research is to study of microbial lipid production by locally photosynthetic microalgae and oleaginous yeast via integrated cultivation technique using CO2 emissions from yeast fermentation. A maximum specific growth rate of Chlorella sp. KKU-S2 of 0.284 (1/d) was obtained under an integrated cultivation and a maximum lipid yield of 1.339g/L was found after cultivation for 5 days, while 0.969g/L of lipid yield was obtained after day 6 of cultivation time by using CO2 from air. A high value of volumetric lipid production rate (QP, 0.
The ubiquinone coenzyme Q (CoQ) is synthesized in mitochondria with a
large, hydrophobic isoprenoid side chain. It functions in mitochondrial res-piration as well as protecting membranes from oxidative damage. Yeast
that cannot synthesize CoQ (DCoQ) are viable, but cannot grow on nonfer-mentable carbon sources, unless supplied with ubiquinone.
Abstract Enterovirus type 71 (EV71) 2A protease exhibited strong transcriptional activity in yeast cells. The transcriptional activity of 2A protease was independent of its protease activity. EV71 2A protease retained its transcriptional activity after truncation of 40 amino acids at the N-terminus but lost this activity after truncation of 60 amino acids at the N-terminus or deletion of 20 amino acids at the C-terminus. Thus, the acidic domain at the C-terminus of this protein is essential for its transcriptional activity.
Yeast IA3 aspartic proteinase inhibitor operates through an unprecedented
mechanism and exhibits a remarkable specificity for one target enzyme, sac-charopepsin. Even aspartic proteinases that are very closely similar to
saccharopepsin (e.g. the vacuolar enzyme fromPichia pastoris) are not sus-ceptible to significant inhibition.
The EVI1 transcriptional repressor is critical to the normal development of
a variety of tissues and participates in the progression of acute myeloid leu-kaemias. The repressor domain (Rp) was used to screen an adult human
kidney yeast two-hybrid library and a novel binding partner designated
ubiquitously expressed transcript (UXT) was isolated.
Alr1p is an integral plasma membrane protein essential for uptake of
into yeast cells. Homologs of Alr1p are restricted to fungi and some
protozoa. Alr1-type proteins are distant relatives of the mitochondrial
and bacterial Mg
-transport proteins, Mrs2p and CorA, respectively, with
which they have two adjacent TM domains and a short Mg
motif in common.
Aspartyl aminopeptidase (EC 18.104.22.168) cleaves only unblocked N-terminal
acidic amino-acid residues. To date, it has been found only in mammals.
We report here that aspartyl aminopeptidase activity is present in yeast.
Yeast aminopeptidase is encoded by an uncharacterized gene in chromo-some VIII ( 1 YHR113W, Saccharomyces Genome Database).
We investigated the changes in the oxidative stress response of yeast cells
suffering mitochondrial dysfunction that could impair their viability.
First, we demonstrated that cells with this dysfunction rely exclusively on
cytosolic thioredoxin peroxidase I (cTPxI) and its reductant sulfiredoxin,
among other antioxidant enzymes tested, to protect them against H2O2
The mechanism of inhibition of yeast F0F1-ATPase by its
naturally occurring protein inhibitor (IF1) was investi-gated in submitochondrial particles by studying the IF1-mediatedATPase inhibition in the presence andabsence
of a protonmotive force. In the presence of protonmotive
force, IF1 added during net NTP hydrolysis almost
completely inhibitedNTPase activity. At moderate IF1
concentration, subsequent uncoupler addition unexpect-edly caused a burst of NTP hydrolysis.