Controlling the impact of stress on brewing biomass, predicting yeast activity and
ensuring consistent fermentation performance through successive fermentations
remain areas of active interest for the brewing industry.
To be able to control and perhaps even manipulate yeast activity, it is necessary to
identify factors that affect its functionality during fermentation. Genetic stability and
integrity are crucial to maintaining predictable performance.
I HAVE selected to-night the particular subject of Yeast for two reasons—or, rather, I should say for three. In the first place, because it is one of the simplest and the most familiar objects with which we are acquainted. In the second place, because the facts and phenomena which I have to describe are so simple that it is possible to put them before you without the help of any of those pictures or diagrams which are needed when matters are more complicated, and which, if I had to refer to them here, would involve the necessity of my...
The summer/winter difference in plasma volume may be related to the regularly observed
summer fall in red blood cell values and total protein. In a fully dehydrated camel with
65% body water, alimentary water has been reported as 12%, which is similar to the
figure for tropical cattle. The total water content of the camel is considerably greater in
hot dry weather (up to 75%) than in cooler periods (down to 50%), which indicates its
physiological water conserving capability.
Economic demands to intensify the brewing process and increase the fermenter
productivity have stimulated interest in high-gravity brewing. However, increasing wort sugar
concentration can have a detrimental effect on fermentation performance, adversely affecting yeast
physiology and altering the physical and flavor properties of the beer product. Many methods such
as: higher pitching rates, higher fermentation temperatures, more efficient aeration than in
conventional brewing, and immobilised yeast were used to improve this process....
For elucidating protein–protein interactions, many methodologies have
been developed during the past two decades. For investigation of interac-tions inside cells under physiological conditions, yeast is an attractive
organism with which to quickly screen for hopeful candidates using versa-tile genetic technologies, and various types of approaches are now avail-able.
Sec14p of the yeastSaccharomyces cerevisiaeis involved in
protein secretion and regulation of lipid synthesis and
turnover in vivo, but acts as a phosphatidylinositol–phos-phatidylcholine transfer proteinin vitro. In this work, the
five homologues of Sec14p, Sfh1p–Sfh5p, were subjected to
biochemical andcell biological analysis toget abetter viewof
their physiological role. We show that overexpression of
SFH2andSFH4suppressed thesec14growth defect in a
more andSFH1in a less efficient way, whereas overexpres-sion ofSFH3andSFH5did not complementsec14....
Transgenic Mice as Models of Genetic Disease
Several organisms have been studied extensively as genetic models, including Mus musculus (mouse), Drosophila melanogaster (fruit fly), Caenorhabditis elegans (nematode), Saccharomyces cerevisiae (baker's yeast), and Escherichia coli (colonic bacterium). The ability to use these evolutionarily distant organisms as genetic models that are relevant to human physiology reflects a surprising conservation of genetic pathways and gene function.
Casein kinases I (CKI) are serine/threonine protein kinases widely expressed in a range of eukaryotes including yeast, mammals and plants. They have been shown to play a role in diverse physiological events including membrane traﬃcking. CKIa is associated with synaptic vesicles and phosphorylates some synaptic vesicle associated proteins including SV2. In this report, we show that syntaxin-1A is phosphorylated in vitro by CKI on Thr21.
The interaction between the human copper(I) chaperone, HAH1, and one
of its two physiological partners, the Menkes disease protein (ATP7A), was
investigated in solution using heteronuclear NMR. The study was carried
out through titrations involving HAH1 and either the second or the fifth
soluble domains of ATP7A (MNK2 and MNK5, respectively), in the pres-ence of copper(I). The copper-transfer properties of MNK2 and MNK5
are similar, and differ significantly from those previously observed for the
yeast homologous system. ...
The humanSUV3gene encodes an NTP-dependent DNA⁄RNA DExH
box helicase predominantly localized in mitochondria. Its orthologue in
yeast is a component of the mitochondrial degradosome complex involved
in the mtRNA decay pathway. In contrast to this, the physiological func-tion of human SUV3remains to be elucidated. In this report we demon-strate that the hSuv3 protein interacts with HBXIP, previously identified as
a cofactor of survivin in suppression of apoptosis and as a protein that
binds the HBx protein encoded by the hepatitis B virus....
We used parameter scanning to emulate changes to the
limitingrate for steps inafittedmodel of glucose-derepressed
yeast glycolysis. Three flux-control regimes were observed,
two of which were under the dominant control of hexose
transport, in accordance with various experimental studies