Luận văn: Nghiên cứu kỹ thuật phát sinh phôi soma và tạo hạt nhân tạo ở cây lan Hồ Điệp (Phalaenopsis sp. ) (part 9)

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Khi thực hiện đề tài này, chúng tôi hy vọng sẽ mang đến những hiểu biết mới về quá trình hình thành phôi vô tính trên cây lan Hồ Điệp nói riêng và hoa lan nói chung, đồng thời đem lại những ứng dụng thiết thực cho ngành nuôi cấy mô thực vật tại Việt Nam. 1.2

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Nội dung Text: Luận văn: Nghiên cứu kỹ thuật phát sinh phôi soma và tạo hạt nhân tạo ở cây lan Hồ Điệp (Phalaenopsis sp. ) (part 9)

81 Thí nghiệm 3 : Ảnh hưởng của chất kích thích sinh trưởng TDZ trong điều kiện môi trường đặc, lỏng lắc đến khả năng hình thành phôi soma từ mô sẹo của cây lan Hồ Điệp in vitro Số phôi tạo ra từ môi trường đặc Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups 188.77344 4 47.193360 38.747 .0000 Within groups 12.18000 10 1.218000 -------------------------------------------------------------------------------- Total (corrected) 200.95344 14 0 missing value(s) have been excluded. Table of means -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- 1 3 .000000 .0000000 .6371813 -1.0041673 1.004167 2 3 2.670000 .5773503 .6371813 1.6658327 3.674167 3 3 4.330000 .1732051 .6371813 3.3258327 5.334167 4 3 10.670000 .5773503 .6371813 9.6658327 11.674167 5 3 5.670000 1.1547005 .6371813 4.6658327 6.674167 -------------------------------------------------------------------------------- Total 15 4.668000 .2849561 .2849561 4.2189227 5.117077 Multiple range analysis -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- 1 3 .000000 X 2 3 2.670000 X 3 3 4.330000 XX 5 3 5.670000 X 4 3 10.670000 X -------------------------------------------------------------------------------- contrast difference limits 1-2 -2.67000 2.00833 * 1-3 -4.33000 2.00833 * 1-4 -10.6700 2.00833 * 1-5 -5.67000 2.00833 * 2-3 -1.66000 2.00833 2-4 -8.00000 2.00833 * 2-5 -3.00000 2.00833 * 3-4 -6.34000 2.00833 * * denotes a statistically significant difference.
82 Số phôi tạo ra từ môi trường lỏng lắc Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups 2931.9868 4 732.99669 261.039 .0000 Within groups 28.0800 10 2.80800 -------------------------------------------------------------------------------- Total (corrected) 2960.0668 14 0 missing value(s) have been excluded. Table of means -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- 1 3 .000000 .0000000 .9674709 -1.524688 1.524688 2 3 4.330000 .5773503 .9674709 2.805312 5.854688 3 3 12.670000 .1154701 .9674709 11.145312 14.194688 4 3 39.670000 1.1547005 .9674709 38.145312 41.194688 5 3 20.000000 1.7320508 .9674709 18.475312 21.524688 -------------------------------------------------------------------------------- Total 15 15.334000 .4326662 .4326662 14.652139 16.015861 Multiple range analysis -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- 1 3 .000000 X 2 3 4.330000 X 3 3 12.670000 X 5 3 20.000000 X 4 3 39.670000 X -------------------------------------------------------------------------------- contrast difference limits 1-2 -4.33000 3.04938 * 1-3 -12.6700 3.04938 * 1-4 -39.6700 3.04938 * 1-5 -20.0000 3.04938 * 2-3 -8.34000 3.04938 * 2-4 -35.3400 3.04938 * 2-5 -15.6700 3.04938 * 3-4 -27.0000 3.04938 * * denotes a statistically significant difference.
83 Thí nghiệm 4 : Ảnh hưởng của các loại môi trường đến quá trình tái sinh cây lan Hồ Điệp in vitro từ phôi soma Tỉ lệ mẫu chết Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups 2481.8963 7 354.55662 47.817 .0000 Within groups 118.6371 16 7.41482 -------------------------------------------------------------------------------- Total (corrected) 2600.5334 23 0 missing value(s) have been excluded. Table of means -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- 1 3 26.670000 .0000000 1.5721341 24.312787 29.027213 2 3 24.446667 2.2233333 1.5721341 22.089454 26.803879 3 3 4.446667 2.2233333 1.5721341 2.089454 6.803879 4 3 2.223333 2.2233333 1.5721341 -.133879 4.580546 5 3 6.670000 .0000000 1.5721341 4.312787 9.027213 6 3 2.223333 2.2233333 1.5721341 -.133879 4.580546 7 3 .000000 .0000000 1.5721341 -2.357213 2.357213 8 3 .000000 .0000000 1.5721341 -2.357213 2.357213 -------------------------------------------------------------------------------- Total 24 8.335000 .5558333 .5558333 7.501599 9.168401 Multiple range analysis -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- 7 3 .000000 X 8 3 .000000 X 4 3 2.223333 XX 6 3 2.223333 XX 3 3 4.446667 XX 5 3 6.670000 X 2 3 24.446667 X 1 3 26.670000 X -------------------------------------------------------------------------------- contrast difference limits 1-2 2.22333 4.71443 1-3 22.2233 4.71443 * 1-4 24.4467 4.71443 * 1-5 20.0000 4.71443 * 1-6 24.4467 4.71443 * * denotes a statistically significant difference.
84 Hệ số nhân chồi Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups 381.55196 7 54.507423 78.569 .0000 Within groups 11.10000 16 .693750 -------------------------------------------------------------------------------- Total (corrected) 392.65196 23 0 missing value(s) have been excluded. Table of means -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- 1 3 .000000 .0000000 .4808846 -.721025 .721025 2 3 .780000 .0577350 .4808846 .058975 1.501025 3 3 1.440000 .2309401 .4808846 .718975 2.161025 4 3 9.000000 1.1547005 .4808846 8.278975 9.721025 5 3 .440000 .1154701 .4808846 -.281025 1.161025 6 3 3.000000 .2886751 .4808846 2.278975 3.721025 7 3 11.340000 .1732051 .4808846 10.618975 12.061025 8 3 5.330000 .5773503 .4808846 4.608975 6.051025 -------------------------------------------------------------------------------- Total 24 3.916250 .1700184 .1700184 3.661329 4.171171 Multiple range analysis -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- 1 3 .000000 X 5 3 .440000 X 2 3 .780000 X 3 3 1.440000 X 6 3 3.000000 X 8 3 5.330000 X 4 3 9.000000 X 7 3 11.340000 X -------------------------------------------------------------------------------- contrast difference limits 1-2 -0.78000 1.44205 1-3 -1.44000 1.44205 1-4 -9.00000 1.44205 * 1-5 -0.44000 1.44205 1-6 -3.00000 1.44205 * * denotes a statistically significant difference.
85 Số lá/chồi Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups 3.9647625 7 .5663946 462.363 .0000 Within groups .0196000 16 .0012250 -------------------------------------------------------------------------------- Total (corrected) 3.9843625 23 0 missing value(s) have been excluded. Table of means -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- 1 3 .0000000 .0000000 .0202073 -.0302982 .0302982 2 3 1.0000000 .0000000 .0202073 .9697018 1.0302982 3 3 1.0000000 .0000000 .0202073 .9697018 1.0302982 4 3 1.2300000 .0173205 .0202073 1.1997018 1.2602982 5 3 1.0000000 .0000000 .0202073 .9697018 1.0302982 6 3 1.2300000 .0346410 .0202073 1.1997018 1.2602982 7 3 1.3800000 .0115470 .0202073 1.3497018 1.4102982 8 3 1.2700000 .0404145 .0202073 1.2397018 1.3002982 -------------------------------------------------------------------------------- Total 24 1.0137500 .0071443 .0071443 1.0030380 1.0244620 Multiple range analysis -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- 1 3 .0000000 X 2 3 1.0000000 X 3 3 1.0000000 X 5 3 1.0000000 X 6 3 1.2300000 X 4 3 1.2300000 X 8 3 1.2700000 X 7 3 1.3800000 X -------------------------------------------------------------------------------- contrast difference limits 1-2 -1.00000 0.06060 * 1-3 -1.00000 0.06060 * 1-4 -1.23000 0.06060 * 1-5 -1.00000 0.06060 * 1-6 -1.23000 0.06060 * * denotes a statistically significant difference.
86 Số rễ/chồi Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups .2386500 7 .0340929 136.371 .0000 Within groups .0040000 16 .0002500 -------------------------------------------------------------------------------- Total (corrected) .2426500 23 0 missing value(s) have been excluded. Table of means -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- 1 3 .0000000 .0000000 .0091287 -.0136873 .0136873 2 3 .0000000 .0000000 .0091287 -.0136873 .0136873 3 3 .0000000 .0000000 .0091287 -.0136873 .0136873 4 3 .0000000 .0000000 .0091287 -.0136873 .0136873 5 3 .0000000 .0000000 .0091287 -.0136873 .0136873 6 3 .2400000 .0230940 .0091287 .2263127 .2536873 7 3 .2200000 .0115470 .0091287 .2063127 .2336873 8 3 .0000000 .0000000 .0091287 -.0136873 .0136873 -------------------------------------------------------------------------------- Total 24 .0575000 .0032275 .0032275 .0526608 .0623392 Multiple range analysis -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- 1 3 .0000000 X 2 3 .0000000 X 3 3 .0000000 X 4 3 .0000000 X 5 3 .0000000 X 8 3 .0000000 X 7 3 .2200000 X 6 3 .2400000 X -------------------------------------------------------------------------------- contrast difference limits 1-2 0.00000 0.02737 1-3 0.00000 0.02737 1-4 0.00000 0.02737 1-5 0.00000 0.02737 1-6 -0.24000 0.02737 * * denotes a statistically significant difference.
87 Chiều dài rễ Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups 66.345863 7 9.4779804 60.659 .0000 Within groups 2.500000 16 .1562500 -------------------------------------------------------------------------------- Total (corrected) 68.845863 23 0 missing value(s) have been excluded. Table of means -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- 1 3 .0000000 .0000000 .2282177 -.3421831 .3421831 2 3 .0000000 .0000000 .2282177 -.3421831 .3421831 3 3 .0000000 .0000000 .2282177 -.3421831 .3421831 4 3 .0000000 .0000000 .2282177 -.3421831 .3421831 5 3 .0000000 .0000000 .2282177 -.3421831 .3421831 6 3 3.6700000 .5773503 .2282177 3.3278169 4.0121831 7 3 4.0000000 .2886751 .2282177 3.6578169 4.3421831 8 3 .0000000 .0000000 .2282177 -.3421831 .3421831 -------------------------------------------------------------------------------- Total 24 .9587500 .0806872 .0806872 .8377700 1.0797300 Multiple range analysis -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- 1 3 .0000000 X 2 3 .0000000 X 3 3 .0000000 X 4 3 .0000000 X 5 3 .0000000 X 8 3 .0000000 X 6 3 3.6700000 X 7 3 4.0000000 X -------------------------------------------------------------------------------- contrast difference limits 1-2 0.00000 0.68437 1-3 0.00000 0.68437 1-4 0.00000 0.68437 1-5 0.00000 0.68437 1-6 -3.67000 0.68437 * * denotes a statistically significant difference.
88 Thí nghiệm 5 : Ảnh hưởng của môi trường tạo vỏ hạt đến sự tái sinh cây con từ hạt nhân tạo Means plot: LSD Confidence level: 95 Range test: LSD Analysis of variance -------------------------------------------------------------------------------- Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level -------------------------------------------------------------------------------- Between groups 4434.6323 5 886.92645 126.704 .0000 Within groups 84.0000 12 7.00000 -------------------------------------------------------------------------------- Total (corrected) 4518.6323 17 0 missing value(s) have been excluded. Table of means -------------------------------------------------------------------------------- Stnd. Error Stnd. Error 95 % LSD Level Count Average (internal) (pooled s) intervals for mean -------------------------------------------------------------------------------- 1 3 44.440000 1.1547005 1.5275252 42.086005 46.793995 2 3 77.780000 1.1547005 1.5275252 75.426005 80.133995 3 3 37.780000 1.1547005 1.5275252 35.426005 40.133995 4 3 55.560000 .5773503 1.5275252 53.206005 57.913995 5 3 71.110000 2.8867513 1.5275252 68.756005 73.463995 6 3 37.780000 1.1547005 1.5275252 35.426005 40.133995 -------------------------------------------------------------------------------- Total 18 54.075000 .6236096 .6236096 53.113986 55.036014 Multiple range analysis -------------------------------------------------------------------------------- Method: 95 Percent LSD Level Count Average Homogeneous Groups -------------------------------------------------------------------------------- 3 3 37.780000 X 6 3 37.780000 X 1 3 44.440000 X 4 3 55.560000 X 5 3 71.110000 X 2 3 77.780000 X -------------------------------------------------------------------------------- contrast difference limits 1-2 -33.3400 4.70799 * 1-3 6.66000 4.70799 * 1-4 -11.1200 4.70799 * 1-5 -26.6700 4.70799 * 1-6 6.66000 4.70799 * 2-3 40.0000 4.70799 * 2-4 22.2200 4.70799 * * denotes a statistically significant difference.