PHLI-seq: Constructing and visualizing cancer genomic maps in 3D by phenotypebased high-throughput laser-aided isolation and sequencing
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Spatial mapping of genomic data to tissue context in a high-throughput and high-resolution manner has been challenging due to technical limitations. Here, we describe PHLI-seq, a novel approach that enables high-throughput isolation and genome-wide sequence analysis of single cells or small numbers of cells to construct genomic maps within cancer tissue in relation to the images or phenotypes of the cells.
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