And medical engineering

The general aims of the present study are to determine the risks involved in acupuncture practice in Victoria, Australia as it is the first state that has systematically registered a non-medical associated treatment regiment and from an analysis of these risks, to develop an integrated framework for clinical risk management of acupuncture.

222p runthenight05 01-03-2023 7 3   Download

Epigenetic defects (gain or loss of DNA methylation) of the human ICR1 11p15 domain result in two opposite foetal growth disorders (BWS and SRS) depending on which parental allele is affected. A few deletions within ICR1 have been reported in familial BWS cases with ICR1 gain of methylation, however the mechanism(s) of the DNA methylation defects at ICR1 remains largely unknown in most BWS and SRS patients.

83p runthenight07 01-03-2023 9 3   Download

The overall aim of this study is to determine the most effective way to reduce the radiation dose to the breast during non-breast related CT scans, whilst still maintaining a suitable image quality. This study will attempt to compare the doses and noise of various protocols that can be used for dose reduction, including tube current modulation, bismuth breast shields and manual reduction of tube current.

111p runthenight07 01-03-2023 11 3   Download

The Arabidopsis FAE1 b-ketoacyl-CoA synthase (FAE1 KCS) catalyzes the condensation of malonyl-CoA with longchain acyl-CoAs. Sequence analysis of FAE1 KCS predicted that this condensing enzyme is anchored to a membrane by two adjacent N-terminal membrane-spanning domains. In order to characterize the FAE1 KCS and analyze its mechanism, FAE1 KCS and its mutants were engineered with a His6-tag at their N-terminus, and expressed in Saccharomyces cerevisiae. The membrane-bound enzyme was then solubilized and purified to near homogeneity on a metal affinity column.

9p research12 01-06-2013 46 3   Download

Human interleukin-11 (hIL-11) is a pleiotropic cytokine that is involved in numerous biological activities such as hematopoiesis, osteoclastogenesis, neurogenesis and female fertility. IL-11 is obviously a key reagent to study the IL-11 receptors. However, conventional radio-iodination techni-ques lead to a loss of IL-11 bioactivity. Here, we report the construction and the production of a new recombinant human IL-11 (FPDIL-11). In this molecule, a speci®c phosphorylation site (RRASVA) has been introduced at the N-terminus of rhIL-11. ...

8p research12 29-04-2013 30 4   Download

The solution structure of three small serine proteinase inhibitors, two natural and one engineered protein, SGCI (Schistocerca gregaria chymotrypsin inhibitor), SGCI[L30R, K31M] and SGTI (Schistocerca gregaria trypsin inhibitor), were determined by homonuclear NMR-spectroscopy. The molecules exhibit di€erent speci®cities towards target proteinases, where SGCI is a good chymo-trypsin inhibitor, itsmutant is apotent trypsin inhibitor, and SGTI inhibits both proteinases weakly.

11p research12 23-04-2013 33 2   Download

Engineered yeast cells efficiently convert ergosta-5-eneol to pregnenolone and progesterone provided that endogenous pregnenolone acetylase activity is disrupted and that heterologous sterolD7-reductase, cytochrome P450 side chain cleavage (CYP11A1) and 3b hydroxysteroid dehydrogenase/isomerase (3b-HSD) activities are present. CYP11A1activity requires the expressionof themammalian NADPH-adrenodoxin reductase (Adrp) and adrenodoxin (Adxp) proteins as electron carriers.

13p tumor12 20-04-2013 28 2   Download

Limited proteolysis in combination with liquid chromatography-ion trap mass spectrometry (LC-MS) was used to analyze engineered or natural proteins derived from a type I modular polyketide synthase (PKS), the 6-deoxyerythronolide B synthase (DEBS), and comprising either the first two extension modules linked to the chain-terminating thioesterase (TE) (DEBS1-TE); or the last two extension modules (DEBS3) or the first exten-sion module linked to TE (diketide synthase, DKS).

15p awards 06-04-2013 38 1   Download

The extreme thermal stabilization achieved by the introduction of a disul-fide bond (G8C⁄N60C) into the cysteine-free wild-type-like mutant (pWT) of the neutral protease fromBacillus stearothermophilus[Mansfeld J, Vri-end G, Dijkstra BW, Veltman OR, Van den Burg B, Venema G, Ulbrich-Hofmann R & Eijsink VG (1997)J Biol Chem 272, 11152–11156] was attributed to the fixation of the loop region 56–69.

12p awards 06-04-2013 43 3   Download

For 30 years, the field of medical device technology has struggled to identify the specifications for optimal biocompatibility. For the vast majority of devices, clinical experience has eventually confirmed that the best performance is achieved when biocom-patibility is equated with biological safety that is materials are selected on the basis of maximizing bioinertness and minimizing toxicity and irritation.

7p inspiron33 26-03-2013 52 5   Download

Kumamolisin-As is an acid collagenase with a subtilisin-like fold. Its active site contains a unique catalytic triad, Ser278-Glu78-Asp82, and a putative transition-state stabilizing residue, Asp164. In this study, the mutants D164N and E78H⁄D164N were engineered in order to replace parts of the catalytic machinery of kumamolisin-As with the residues found in the equivalent positions in subtilisin.

14p inspiron33 26-03-2013 54 5   Download

Human manganese superoxide dismutase is a mitochondrial metalloenzyme that is involved in protecting aerobic organisms against superoxide toxicity, and has been implicated in slowing tumor growth. Unfortunately, this enzyme exhibits strong product inhibition, which limits its potential bio-medical applications.

9p inspiron33 23-03-2013 42 3   Download

A fatty-acid-metabolizing enzyme fromArabidopsis thaliana, CYP94C1, belonging to the cytochrome P450 family was cloned and characterized. CYP94C1 was heterologously expressed in aSaccharomyces cerevisiae strain (WAT11) engineered for P450 expression. When recombinant yeast microsomes were incubated with lauric acid (C12:0) for 15 min, one major metabolite was formed.

12p media19 04-03-2013 42 2   Download

It remains a challenge to achieve the stable and long-term expression (in human cell lines) of a previously engineered hybrid enzyme [triple-catalytic (Trip-cat) enzyme-2; Ruan KH, Deng H & So SP (2006) Biochemistry45, 14003–14011], which links cyclo-oxygenase isoform-2 (COX-2) to prostacy-clin (PGI2) synthase (PGIS) for the direct conversion of arachidonic acid into PGI2through the enzyme’s Trip-cat functions.

10p vinaphone15 28-02-2013 37 3   Download

A set of thirty-two natural and ten semisynthetic ecdysteroids was assayed in murine 3T3 cells across ten different ecdysteroid receptor (EcR) ligand-binding domains derived from nine arthropod species in an engineered gene switch format. Among the ecdysteroids tested, the most biologically wide-spread ecdysteroid, 20-hydroxyecdysone (20E), was moderately and consis-tently potent across the tested EcRs.

25p vinaphone15 28-02-2013 33 2   Download

l-Asparaginase (EC 3.5.1.1,l-ASNase) catalyses the hydrolysis ofl-Asn, producingl-Asp and ammonia. This enzyme is an anti-neoplastic agent; it is used extensively in the chemotherapy of acute lymphoblastic leukaemia. In this study, we describe the use of in vitro directed evolution to create a new enzyme variant with improved thermal stability.

12p vinaphone15 27-02-2013 53 4   Download

Experimental design has a long tradition in statistics, engineering and life sciences, dating back to the beginning of the last century when optimal designs for industrial and agricultural trials were considered. In cell biol-ogy, the use of mathematical modeling approaches raises new demands on experimental planning.

17p vinaphone15 27-02-2013 40 3   Download

Elucidating the origin of substrate and coenzyme specificity has been the focus of much work relating to enzyme engineering. Many enzymes exhibit tight specificity for particular substrates despite a close structural relation-ship to other nonreactive compounds.

10p vinaphone15 25-02-2013 38 3   Download

The active site of triosephosphate isomerase (TIM, EC: 5.3.1.1), a dimeric enzyme, lies very close to the subunit interface. Attempts to engineer monomeric enzymes have yielded well-folded proteins with dra-matically reduced activity.

15p vinaphone15 25-02-2013 38 3   Download

KcsA, a potassium channel fromStreptomyces lividans, was the first ion channel to have its transmembrane domain structure determined by crystal-lography. Previously we have shown that its C-terminal cytoplasmic domain is crucial for the thermostability and the expression of the channel. Expression was almost abolished in its absence, but could be rescued by the presence of an artificial left-handed coiled coil tetramerization domain GCN4.

11p viettel02 20-02-2013 47 3   Download