Antigen and antibody

We evaluated the immunoreactivity profiles of eight commercial anti-host cell protein (anti-HCP) antibodies from different host animals and their antigens used for immunization by an isobaric labeled affinity purification-mass spectrometry (AP-MS) method.

9p vironald 15-12-2022 9 1   Download

Haemonchus contortus (H. contortus) is one of the most important parasites that cause huge economic losses to small ruminant industry worldwide. Effective prognosis and treatment depend upon the early diagnosis of H. contortus infection.

11p vigalileogalilei 28-02-2022 16 1   Download

The aim of the study was to determine the prevalence of laboratory abnormalities suggestive of kidney disease in clinically healthy BMDs compared to a control population and to investigate if there is a correlation with the occurrence of antibodies to B. burgdorferi sensu lato, Ehrlichia canis, and Anaplasma (A.) spp. and with the occurrence of Dirofilaria (D.) immitis antigen.

8p vigandhi 23-02-2022 8 1   Download

Bacillus anthracis is the causative agent of anthrax, a disease of both humans and various animal species, and can be used as a bioterror agent. Effective vaccines are available, but those could benefit from improvements, including increasing the immunity duration, reducing the shot frequency and adverse reactions. In addition, more sophisticated antigen delivery and potentiation systems are urgently required.

10p vigandhi 23-02-2022 7 1   Download

Mutant peptides presented by MHC (major histocompatibility complex) Class II in cancer are important targets for cancer immunotherapy. Both animal studies and clinical trials in cancer patients showed that CD4 T cells specific to tumor-derived mutant peptides are essential for the efficacy of immune checkpoint blockade therapy by PD1 antibody.

9p vitzuyu2711 29-09-2021 5 0   Download

Natural antibodies (NAb) are an important component of the innate immune system, and fight infections as a part of the first line defence. NAb are poly-reactive and can respond non-specifically to antigens.

11p vitzuyu2711 29-09-2021 9 0   Download

B cell affinity maturation enables B cells to generate high-affinity antibodies. This process involves somatic hypermutation of B cell immunoglobulin receptor (BCR) genes and selection by their ability to bind antigens.

11p vijeeni2711 30-06-2021 6 0   Download

NS1 antigen, known as Non Structural antigen is an important antigenic component of Dengue Virus. Dengue virus is transmitted by bite of Aedes aegyptii mosquito. Hemorrhagic manifestations and shock syndrome are most dreaded complications of Dengue IgM antibody is produced in Acute phase and IgG in later phases of Dengue and may remain for very long periods.

4p trinhthamhodang11 27-04-2021 12 0   Download

Previous studies have suggested that antibodies against human leukocyte antigen (HLA) are associated with worse outcomes in lung transplantation. However, little is known about the factors associated with outcomes following lung transplantation in Asia.

9p vimaine2711 26-03-2021 8 1   Download

The immune checkpoint receptor programmed cell death protein I (PD-1) has been identified as a key target in immunotherapy. PD-1 reduces the risk of autoimmunity by inducing apoptosis in antigen-specific T cells upon interaction with programmed cell death protein ligand I (PD-L1).

12p viwyoming2711 16-12-2020 7 1   Download

Recombinant human CD45RO antigen was expressed from E. coli BL21 (DE3), purified and analyzed by SDS-PAGE and Western blotting. The purified CD45RO antigen was used to immunize Balb/c mice. Spleen cells from immunized mouse were collected and fused with P3X63Ag8.653 myeloma cells to form hybridoma. Anti-CD45 antibody-secreting capacity of hybridoma clones was evaluated by ELISA assay. Anti-CD45 MAb from the culture supernatant of the chosen hybridoma clone was purified by affinity chromatography. The MAb was characterized the biochemical characteristics and biological activity.

8p trinhthamhodang9 04-12-2020 7 1   Download

Today’s modern research of B and T cell antigen receptors (the immunoglobulins (IG) or antibodies and T cell receptors (TR)) forms the basis for detailed analyses of the human adaptive immune system.

10p vikentucky2711 24-11-2020 4 0   Download

The genes that produce antibodies and the immune receptors expressed on lymphocytes are not germline encoded; rather, they are somatically generated in each developing lymphocyte by a process called V(D)J recombination, which assembles specific, independent gene segments into mature composite genes. The full set of composite genes in an individual at a single point in time is referred to as the immune repertoire.

10p vioklahoma2711 19-11-2020 4 0   Download

The humoral immune system response is based on the interaction between antibodies and antigens for the clearance of pathogens and foreign molecules. The interaction between these proteins occurs at specific positions known as antigenic determinants or B-cell epitopes.

7p vioklahoma2711 19-11-2020 3 1   Download

The identification of immunogenic regions on the surface of antigens, which are able to be recognized by antibodies and to trigger an immune response, is a major challenge for the design of new and effective vaccines. The prediction of such regions through computational immunology techniques is a challenging goal, which will ultimately lead to a drastic limitation of the experimental tests required to validate their efficiency.

12p vioklahoma2711 19-11-2020 7 2   Download

The single chain fragment antibody (scFv) antibodies were raised against CRY2B antigen by phage display technology. In antigen-antibody affinity selection the scFv antibody displaying phages concentration was increased (4.54 × 1010 to 2.3 ×1011) and fourth round phages were higher in concentration and affinity (1.06) to antigen revealed by ELISA.

11p nguaconbaynhay8 13-10-2020 10 0   Download

In 2009, a novel influenza vaccine was distributed worldwide to combat the H1N1 influenza “swine flu” pandemic. However, antibodies induced by the vaccine display differences in their specificity and cross-reactivity dependent on pre-existing immunity.

13p vicolorado2711 22-10-2020 6 0   Download

Rabies is a highly fatal viral infection of central nervous system affecting all warm blooded animals including humans. The virus belongs to the Rhabdoviridae family, genus Lyssa virus. Several techniques have been standardized internationally for the diagnosis of rabies. This study was designed to assess the efficacy of Seller's staining test (SST) and Direct Fluorescent Antibody Test (dFAT) for diagnosis of rabies antigen, Mouse Innoculation Test (MIT) and real time reverse transcriptase polymerase chain reaction (qRT-PCR) were used as the Gold Standards.

7p trinhthamhodang1215 23-09-2020 7 0   Download

The present study has been conducted in the rural population attended to MNR medical college for blood donation. Among the 100 voluntary donors screened for HIV the mean age group being taken 19 to 50 yrs. Among the gender the male donors were more in number.

11p trinhthamhodang1215 23-09-2020 10 0   Download

Prostate cancer (PCa) is the second leading cause of cancer deaths in men in the United States. The prostate-specific antigen (PSA), often found at high levels in the serum of PCa patients, has been used as a marker for PCa detection and as a target of immunotherapy.

13p vijennie2711 29-09-2020 13 0   Download