Biochemical tests

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  • This book is designed to fit into a handbag or shirt pocket. It contains material which is usually required in a hurry when treating a critically ill or injured infant or child. In addition to information relevant to emergency care, there are what we hope will be useful pages of data on subjects which without a photographic memory would normally require reference to a paediatric text book. Examples include a weight for height chart, normal values for common biochemical tests, normal developmental profiles and normal ECG measurements....

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  • A plant nutrient is a chemical element that is essential for plant growth and reproduction. Essential element is a term often used to identify a plant nutrient. The term nutrient implies essentiality, so it is redundant to call these elements essential nutrients. Commonly, for an element to be a nutrient, it must fit certain criteria. The principal criterion is that the element must be required for a plant to complete its life cycle. The second criterion is that no other element substitutes fully for the element being considered as a nutrient.

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  • Methylation was initially addressed as one of the most primitive mechanisms that organisms utilize to (a) protect genomic DNA and initiate the host resistance mechanism towards foreign DNA insertion and subsequently, (b) control gene expression, (Doerfler & Böhm, 2006). From an evolutionary point of view as well, the catalytic domain in the structure of the methylation enzymes across all organisms has been preserved to perform methyl group addition.

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  • The programme is indebted to the five hundred plus international nurses from twentythree different countries who have worked and studied with us over the last four years at the Oxford Radcliffe Hospitals NHS Trust, the University Hospitals of Leicester NHS Trust and other hospitals. They have tested our new ideas, suggested improvements and shown honesty, courage and a sense of humour throughout. We are particularly grateful to the international nurses who commented on the first drafts of this book....

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  • Chapter 004. Screening and Prevention of Disease Examples include tests that are biochemical (e.g., cholesterol, glucose), physiologic (e.g., blood pressure, growth curves), radiologic (e.g., mammogram, bone densitometry), or tissue specimens (e.g., Pap smear, fine-needle aspirations). In the future, it is anticipated that genetic testing will play an increasingly important role for predicting disease risk (Chap. 64). However, such tests are not widely used except for individuals at risk for high-penetrance genes based on family or ethnic history (e.g., BRCA1, BRCA2).

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  • PCR detection of viral pathogens is extremely useful, but suffers from the challenge of detecting the many variant strains of a given virus that arise over time. Here, we report the computational derivation and initial experi-mental testing of a combination of 10 PCR primers to be used in a single high-sensitivity mixed PCR reaction for the detection of dengue virus.

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  • The positive cofactor 4 (PC4) protein has an important role in transcrip-tional activation, which has been proposed to be mediated by transcription factor IIA (TFIIA) and TATA-binding protein-associated factors. To test this hypothesis, we cloned the Schizosaccharomyces pombe PC4gene and analysed the role of the PC4 protein in the stimulation of basal tran-scription driven by TATA-containing and TATA-less promoters.

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  • Microvascular perfusion is often impaired after primary percutaneous coronary intervention (PCI). We proposed that in situ thrombosis might contribute to poor myocardial perfusion in this setting. To test this hypothesis, we evaluated the effect of lowdose intracoronary streptokinase administered immediately after primary PCI. Methods Forty-one patients undergoing primary PCI were randomly assigned to receive intracoronary streptokinase (250 kU) or no additional therapy.

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  • Although dyspnea is a common symptom, there has been only limited investigation of its prognostic significance among patients referred for cardiac evaluation. methods We studied 17,991 patients undergoing myocardial-perfusion single-photon-emission computed tomography during stress and at rest. Patients were divided into five categories on the basis of symptoms at presentation (none, nonanginal chest pain, atypical angina, typical angina, and dyspnea).

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  • The concentrations of two structurally distinct membrane fluidizers, the local anesthetic benzyl alcohol (BA) and heptanol (HE), were used at con-centrations so that their addition to K562 cells caused identical increases in the level of plasma membrane fluidity as tested by 1,6-diphenyl-1,3,5-hexa-triene (DPH) anisotropy.

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  • SUT2was found ina screen formulticopy suppressorsof the synthetic slow growth phenotype of aDras2Dgpa2double deletion mutant. It failed, however, to cure the lethal phenotype of aDras1Dras2mutant suggesting that it acts upstreamof Ras or in a parallel pathway. By testing cAMP-dependent reactions including the accumulation of storage carbohydrates, pseudohyphal differentiation, entry of mei-osis as well as the measurement ofFLO11reporter activity we show that Sut2pmodulates the activity of protein kinase A (PKA)....

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  • (BQ) Part 1 book "Clinical Biochemistry" presents the following contents: Biochemical investigations and quality control, automation, kidney disease, hyperuricaemia and gout, fluid and electrolyte disorders, clinical enzymology and biomarkers, liver function tests,... and other contents.

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  • The assembly status of the cytochromebc1 complex has been analyzed in distinct yeast deletion strains in which genes for one or more of thebc1 subunits were deleted. In all the yeast strains tested, a bc1 sub-complex of approximately 500 kDa was found when the mitochondrial membranes were analyzed by blue native electrophoresis.

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  • Hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) are the most common hereditary cardiac conditions. Both are frequent causes of sudden death and are often associated with an adverse disease course.Alpha-cardiac actinis one of the disease genes where different mis-sense mutations have been found to cause either HCM or DCM. We have tested the hypothesis that the protein-folding pathway plays a role in dis-ease development for two actin variants associated with DCM and six asso-ciated with HCM....

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  • Anovel photoactivatable analog of antisauvagine-30 (aSvg-30), a specific antagonist for corticotropin-releasing factor (CRF) receptor, type 2 (CRF2), has been synthesized and characterized. TheN-terminal amino-acidD-Phe in aSvg-30 [D-Phe11,His12]Svg(11)40) was replacedbyaphenyldiazirine, the 4-(1-azi-2,2,2-trifluoroethyl)benzoyl (ATB) residue.

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  • Recently, we have identified and purified minisatellite DNA binding proteins (MNBPs) that bind to the mouse hypervariable minisatellite Pc-1, from NIH3T3 cells. This study describes the isolation and characterization of a mouse leucine-rich protein (mLRP130) as one of the MNBPs that binds to the C-rich strand of Pc-1. The mLRP130 cDNA was demonstrated to encode a polypeptide of 1306 aminoacid residues with a deduced molecular mass of 137 kDa, and the mLRP130 mRNA is detected in various organs, including heart, brain, liver, skeletal muscle, kidneys and testes.

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  • The identity of an organism may be confirmed by demonstrating its ability to perform a number of biochemical reactions, each species conforming to a recognizable result pattern. Most of the media used in the tests detailed in this section can be obtained from commercial sources

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  • Harrison's Internal Medicine Chapter 4. Screening and Prevention of Disease Screening and Prevention of Disease: Introduction A primary goal of health care is to prevent disease or to detect it early enough that intervention will be more effective. Strategies for disease screening and prevention are driven by evidence that testing and intervention are practical and effective. Currently most screening tests are readily available and inexpensive. Examples include tests that are biochemical (e.g., cholesterol, glucose), physiologic (e.g., blood pressure, growth curves), radiologic (e.g.

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  • Vitamins and trace minerals are required constituents of the human diet since they are either inadequately synthesized or not synthesized in the human body. Only small amounts of these substances are needed for carrying out essential biochemical reactions (e.g., acting as coenzymes or prosthetic groups). Overt vitamin or trace mineral deficiencies are rare in Western countries due to a plentiful, varied, and inexpensive food supply; however, multiple nutrient deficiencies may appear together in persons who are chronically ill or alcoholic.

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  • Cytogenetic Testing in Prenatal Diagnosis The vast majority of prenatal diagnostic studies are performed to rule out a chromosomal abnormality, but cells may also be propagated for biochemical studies or molecular analyses of DNA. Three procedures are used to obtain samples for prenatal diagnosis: amniocentesis, chorionic villus sampling (CVS), and fetal blood sampling. Amniocentesis is the most common procedure and is routinely performed at 15–17 weeks of gestation.

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