Biology and culture

This project is focused on studying the biological effects of low-power RF radiation on the expression and function of mechanosensitive ion channel protein (Piezo-1). To evaluate the effects of long-term exposures, cells were exposed at the frequency of 1800 MHz and power of 17 dBm for a period of 2 and 4 hrs using the transverse electromagnetic (TEM) cell exposure system. To study the effects of short-term RF radiation (10 min), exposures emitted from the mobile phone headset at the frequency of 845 MHz and powers of -15 dBm and -5 dBm were applied to cell cultures.

141p runthenight04 02-02-2023 9 3   Download

Objectives of the study: Survey of biological activity of alkaloid isolated from hairy roots of E.longifolia to find new activities. Determining optimal culture conditions for E.longifolia hairy roots to grow and develop. Determining the concentration of three elicitors (Salicylic acid, Jasmonic acid and Yeat extract) on the production and accumulation of alkaloids isolated from hairy roots of E.longifolia in vitro.

26p extraenglish 24-05-2021 30 5   Download

The objective of the topic: The objective of this project is determination the role of a number of factors such as LED light, type and concentration of sugar, plant growth regulators, mineral medium, amino acids and polyamine in the flowering of T.fournieri in vitro.

27p extraenglish 24-05-2021 32 5   Download

The objective of the study was to find the explant resources, the type and concentration of plant growth regulators (PGRs), as well as the in vitro culture conditions suitable for different morphogenesis processes - callus induction, direct embryogenesis, shoot and root formation, etc. The growth and development of in vitro Ngoc Linh ginseng derived from thin cell layer (TCL) was examined in Quang Nam to assess the adaptability of in vitro Ngoc Linh plantlets in its natural territory, compared to those growing in Bidoup - Nui Ba National Park, Lam Dong.

27p capheviahe27 23-02-2021 21 5   Download

To provide scientific data on biological characteristics, breeding techniques, nursing and grow-out of Helmet Catfish - Cranoglanis bouderius (Richardson, 1846) in culture condition of Nghe An province. The success of the thesis will greatly contribute the basic knowledge to develop the seed production technique that can help to support the seed resources for commercial culture models and gene resources conservation for this species.

28p capheviahe27 23-02-2021 16 4   Download

The objective of this project is determination the role of a number of factors such as LED light, type and concentration of sugar, plant growth regulators, mineral medium, amino acids and polyamine in the flowering of T. fournieri in vitro.

27p larachdumlanat129 20-01-2021 36 5   Download

Based on the above scientific bases and arguments, I conduct a research project to search for bioactive alkaloids while also researching new activities from alkaloids, thereby increasing cumulative growth culture. Content of alkaloids by adding elicitors.

26p larachdumlanat129 20-01-2021 33 4   Download

To identify species, biological traits, chemical composition, and bioactive activities of essential oil and to develop propagation technical process using tissue culture techniques from shoot bud cutting to contribute to the conservation and development of genetic sources of native ginger plants in Bac Kan.

27p trinhthamhodang7 31-08-2020 46 2   Download

The study was carried out during 2013-14 in Meerut district of Uttar Pradesh state to focus on the sugarcane growers regarding impact of knowledge on adoption level of IPM practices. The 80 contact farmers were selected as respondents. The study revealed that the maximum knowledge gap was found to be existing in previous crop residues, crop rotation, hand picking of the insect and their destruction, hot air and water treatment, light and pheromone trap, microbial control, use of natural enemies, resistant varieties, biopesticides/ bio-agents, application and dose of different pest etc.

7p chauchaungayxua6 26-06-2020 21 3   Download

The objective of the study was to find the explant resources, the type and concentration of plant growth regulators (PGRs), as well as the in vitro culture conditions suitable for different morphogenesis processes - callus induction, direct embryogenesis, shoot and root formation, etc. The growth and development of in vitro Ngoc Linh ginseng derived from thin cell layer (TCL) was examined in Quang Nam to assess the adaptability of in vitro Ngoc Linh plantlets in its natural territory, compared to those growing in Bidoup - Nui Ba National Park, Lam Dong.

27p xacxuoc4321 09-07-2019 33 5   Download

The general objectives of the study are to evaluate the status and to find out scientific basis to develop freshwater prawn culture in brackish water, in order to diversify culture systems and species, to stabilize social-economics for inhabitants, and to adapt to the issues of sea level rise and saltwater intrusion in Mekong Delta.

25p change05 08-06-2016 41 3   Download

The octadecaneuropeptide (ODN; QATVGDVNTDRPG LLDLK) and its C-terminal octapeptide (OP; RPGLLDLK), which exert anxiogenic activity, have been previously shown to increase intracellular calcium concentration ([Ca21]i) in cultured rat astrocytes through activation of a metabotropic receptor positively coupled to phospholipase C. It has also been found that the [D -Leu5]OP analog possesses a weak antagonistic activity. The aim of the present study was to synthesize and characterize cyclic analogs of OP and [D -Leu5]OP.

13p system191 01-06-2013 32 4   Download

The spermine analogue N1,N11-diethylnorspermine (DENSPM) efficiently depletes the cellular pools of putrescine, spermidine and spermine by down-regulating the activity of the polyamine biosynthetic enzymes and up-regulating the activity of the catabolic enzyme spermidine/ spermine N1-acetyltransferase (SSAT). In the breast cancer cell line L56Br-C1, treatment with 10 lM DENSPM induced SSAT activity 60 and 240-fold at 24 and 48 h after seeding, respectively, which resulted in polyamine depletion. ...

7p research12 01-06-2013 53 5   Download

Two secreted acid phosphatases (SAP1 and SAP2) were markedly up-regulated during Pi-starvation of tomato suspension cells.SAP1 and SAP2 were resolved during cat-ion-exchange FPLC of culture media proteins from 8-day-old Pi -starved cells, and purified to homogeneity and final p-nitrophenylphosphatehydrolyzing specificactivitiesof 246 and 940lmol Pi producedÆmin )1 mgÆprotein )1 , respect-ively.SDS/PAGE, periodic acid-Schiff staining and analyt-ical gel filtration demonstrated that SAP1 and SAP2, respectively, exist as 84 and57 kDa glycosylatedmonomers....

9p tumor12 22-04-2013 55 2   Download

PhotobacteriumphosphoreumIFO13896 emits light strongly when cultured in medium containing 3% NaCl, but only weakly in medium containing 1% NaCl.It is known that dim or dark mutants appear frequently and spontaneously from this parent strain.To confirm that riboflavin biosyn-thesis is stimulatedwhen theluxoperon is active, the amount of light emitted and flavins synthesized under strongly or weakly light emitting conditions was determined.

10p tumor12 22-04-2013 27 5   Download

To identify all of theO-methyltransferase genes involved in isoquinolinealkaloidbiosynthesis inCoptis japonicacells,we sequenced 1014 cDNAclones isolated from high-alkaloid-producing cultured cells ofC. japonica.Amongthem,we found all three reported O-methyltransferases and an O-methyltransferase-likecDNAclone (CJEST64).This cDNA was quite similar toS-adenosyl-L-methionine:coclaurine 6-O-methyltransferase and S-adenosyl-L-methionine:iso-flavone 7-O-methyltransferase.

9p tumor12 22-04-2013 26 3   Download

Primary olfactory neuronal cultures exposed to odorant stimulation have previously exhibited concentration-related effects in terms of intracellular cAMP levels and adenylate cyclase activity [Ronnett, G.V., Parfitt, D.J., Hester, L.D. & Snyder, S.H. (1991)PNAS88, 2366–2369].Maximal sti-mulation occurred for intermediate concentrations, whereas AC activity declined for both low and high odorant con-centrations.We suspected that this behavior might be ascribed to the intrinsic response of the first molecular species concerned by odorant detection, i.e. the olfactory receptor itself....

8p fptmusic 16-04-2013 37 3   Download

A thermostable enzyme from the hyperthermophilic sul-phate-reducing archaeon, Archaeoglobus fulgidus,was expressed and characterized on the assumption that it is homologous to exonuclease III from Escherichia coli. Sequence similaritydatabase searcheswere performedbased on the amino acid sequence of exonuclease III. The 774bp longgenewas isolated froma culture sample andcloned into different vectors.

12p fptmusic 16-04-2013 27 3   Download

To test the role of poly(ADP-ribose) polymerase on the telomerase activity, we determined the telomerase activity in leukemic cells K562 treated with benzamide and 4-amino 1,8 naphthalimide (NAP), the inhibitors of PARP. We observed that both the agents inhibited telomerase activity in a dose-dependent manner. The doses of benzamide and NAP that inhibited telo-merase activity to 50% of untreated control cells were 10.7 ± 0.6 mmand 200 ± 7lm, respectively.

12p fptmusic 11-04-2013 47 2   Download

GE23077, a novel microbial metabolite recently isolated from Actinomadurasp. culture media, is a potent and selective inhibitor of bacterial RNApolymerase (RNAP). It inhibitsGram-positive (Bacillus subtilis) andGram-negative (Escherichia coli)RNAPs with IC50 values (i.e. the concen-tration at which the enzyme activity is inhibited by 50%) in the 10 )8 Mrange, whereas it is not active onE. coliDNA polymerase or on eukaryotic (wheat germ) RNAP II (IC50 values10 )4 Min both cases).

0p awards 05-04-2013 56 2   Download