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Blunt vs sticky ends

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  • Once you have your restriction enzymes chosen, it is time to design the final complete gene The multiple cloning site (or whatever plasmid you are cloning into) should already have the 5’ portion of the gene intact (i.e. RBS, spacer, Met) Sequences must be in frame

    ppt34p taytrang001 04-12-2012 43 6   Download

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