Confocal microscopy

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  • Science often grows exponentially from certain key insights, and the insight Marvin Minsky had in 1955 is no exception. While working as a postdoctoral fellow at HarvardUniversity, Minsky sought a method to reduce the high level of light scatter that made high contrast imag‐ ing of thick specimens impossible by standard light microscopy.

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  • Using an NSOM for confocal microscopy actin in the brain, nanopositioning, role of glial cells and actin in the brain,... As the main contents of the document "Role of precision motion control in brain cell research". Invite you to consult

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  • Microscopic and imaging techniques: – Optical microscopy – Confocal microscopy – Electron microscopy (SEM and TEM, related methods) – Scanning probe microscopy (STM and AFM, related methods) Surface spectrometric techniques: – X-ray fluorescence (from electron microscopy) – Auger electron spectrometry – X-ray photoelectron spectrometry (XPS/UPS/ESCA)

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  • Tham khảo sách 'laser scanning, theory and applications_1', kỹ thuật - công nghệ, điện - điện tử phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả

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  • Ever since the invention of laser by Schawlow and Townes in 1958, various innovative ideas of laser-based applications emerge very year. At the same time, scientists and engineers keep on improving laser's power density, size, and cost which patch up the gap between theories and implementations. More importantly, our everyday life is changed and influenced by lasers even though we may not be fully aware of its existence.

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  • Non-invasive Breslow index determination would allow one-time procedure for melanoma clinical management which would be of utmost medical interest for several reasons: unimpaired sentinel lymph node status, low morbidity, pain and stress associated to surgical excision margins. However, as explained throughout the current chapter, such a determination requires melanoma diagnosis prior to Breslow determination.

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  • Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Spontaneous confocal Raman microscopy–a tool to study the uptake of nanoparticles and carbon nanotubes into cells

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  • Our goal is to develop automated methods for the segmentation of threedimensional biomedical images. Here, we describe the segmentation of confocal microscopy images of bee brains (20 individuals) by registration to one or several atlas images. Registration is performed by a highly parallel implementation of an entropy-based nonrigid registration algorithm using B-spline transformations. We present and evaluate different methods to solve the correspondence problem in atlas based registration.

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  • In 1665, a book was published that inaugurated the use of the microscope to investigate the natural world. The author was Robert Hooke, a talented artist, architect, and amateur scientist. Hooke wrote Micrographia: Or Some Physiological Descriptions of Minute Bodies Made by Magnifying Glasses with Observations and Inquiries Thereupon, at the behest of the newly chartered Royal Society in London, for whom he was working as curator of scientific experiments. In Micrographia, he presented the first detailed observations of everyday objects made with his self-constructed light microscope....

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  • We analyzed the role of gangliosides in the association of the ErbB2 recep-tor tyrosine-kinase (RTK) with lipid rafts in mammary epithelial HC11 cells. Scanning confocal microscopy experiments revealed a strict ErbB2– GM3 colocalization in wild-type cells. In addition, analysis of membrane fractions obtained using a linear sucrose gradient showed that ErbB2, epi-

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  • Tham khảo sách 'confocal laser microscopy - principles and applications in medicine, biology, and the food sciences by lagali contributors', y tế - sức khoẻ, y học thường thức phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả

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  • Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" In vivo imaging of the airway wall in asthma: fibered confocal fluorescence microscopy in relation to histology and lung function...

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  • Jurkat T lymphocytes were treated with daunorubicin and WP631, a daunorubicin-based DNAbinding agent, in experiments aimed to analyze cellular uptake of these drugs and their effect on cell viability. WP631 was taken up more slowly than daunorubicin, but laser confocal microscopy and spectrofluorometricquantificationshowed that thedrug accumulated in the cells. Despite the slow uptake rate, the antiproliferative capacity of WP631 (measured as IC50after a 72-h continuous treatment) was greater than that of daunorubicin.

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  • Inducible nitric oxide synthase (iNOS) functions as a homodimer. In cell extracts, iNOS molecules partition both in cytosolic and particulate frac-tions, indicating that iNOS exists as soluble and membrane associated forms. In this study, iNOS features were investigated in human intestinal epithelial cells stimulated with cytokines and in duodenum from mice exposed to flagellin. Our experiments indicate that iNOS is mainly associ-ated with the particulate fraction of cell extracts.

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  • The relationships between cardiac cell structure and the regulation of mitochondrial respiration were studied by applying fluorescent confocal microscopy and analysing the kinetics of mitochondrial ADP-stimulated respiration, during calcium-induced contraction in permeabilized cardiomyo-cytes and myocardial fibers, and in their ‘ghost’ preparations (after selective myosin extraction).

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  • In the eight years since publication of the first edition, enormous progress has been made in the field of skin imaging and analysis. Because of the broad array of new methods now available to the research scientist—and more and more to the clinically- oriented dermatologist—we were able to widen the scope of this book from the skin surface to the entire skin. This is also reflected in the change of the volume title, Bioengineering of the Skin: Skin Imaging and Analysis. Hence, this second edition is a major revision of the first edition, with more than 30 new chapters added....

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  • TRPM4b is a Ca 2+ -activated, voltage-dependent monovalent cation chan-nel that has been shown to act as a negative regulator of Ca 2+ entry and to be involved in the generation of oscillations of Ca 2+ influx in Jurkat T-lymphocytes. Transient overexpression of TRPM4b as an enhanced green fluorescence fusion protein in human embryonic kidney (HEK) cells resulted in its localization in the plasma membrane, as demonstrated by confocal fluorescence microscopy.

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  • The uptake by mammalian cells of phosphorothioate oli-gonucleotides was compared with that of their respective complexes or conjugates with cationic, cell-penetrating model peptides of varying helix-forming propensity and amphipathicity. An HPLC-based protocol for the synthesis and purification of disulfide bridged conjugates in the 10– 100 nmol range was developed. Confocal laser scanning microscopy (CLSM) in combination with gel-capillary electrophoresis and laser induced fluorescence detection (GCE-LIF) revealed cytoplasmic and nuclear accumula-tion in all cases. ...

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