Cycling and cycling indices

Orexin A and B (also known as hypocretins), two recently discovered neuropeptides, play an important role in food intake, sleep/wake cycle and neuroendocrine functions. Orexins are endogenous ligands of two G-protein-coupled receptors, termed OX1 and OX2. This work presents the first short orexin A and B analogues, orexin A 23–33 and orexin B 18–28, with high affinity (119 ± 49 and 49 ± 23 nM) for OX1 receptors expressed on SK-N-MC cells and indicates the importance of the C-terminal part of the orexin peptides for this ligand–receptor interaction.

8p research12 01-06-2013 33 5   Download

Ribonucleotide reductase is essential for the synthesis of all four dNTPs required for DNA replication. The enzyme is composed of two proteins, R1 and R2, which are both needed for activity. Expression of the R1 andR2mRNAs is restricted to the S-phase of the cell cycle, but the R1 andR2 promoters shownoobvious sequence homologies that could indicate coordination of transcription.

11p fptmusic 16-04-2013 36 3   Download

Metabolically unstable proteins are involved in a multitude of regulatory networks, including those that control cell signaling, the cell cycle and in many responses to physiological stress. In the present study, we have deter-mined the stability and characterized the degradation process of some members of the Gq class of heterotrimeric G proteins. Pulse-chase experi-ments in HEK293 cells indicated a rapid turnover of endogenously expressed Gaq and overexpressed Gaq and Ga16 subunits.

13p fptmusic 12-04-2013 37 2   Download

They clam approximately 500 species, widely distributed in coastal intertidal zone of tropical temperate countries. Our country has about 40 species of 7 breed groups, distributed along the coast from north to south. Northern coastal areas have clam oil (Meretrix lime), clam password (Meretrix llusoria Rumplius). Southern coastal areas have clam (Meretrix lyrata Sowerby). Clams are mollusks team has great potential in the tide country. Culture technique is not complicated, short cycle, investment less valuable export.

46p xinh_la 10-02-2012 71 9   Download

Functional cooperation between FACT and the MCM helicase complex constitutes an integral step during DNA replication initiation. However, mode of regulation that underlies the proper functional interaction of FACT and MCM is poorly understood. Methods & Results: Here we present evidence indicating that such interaction is coordinated with cell cycle progression and differential complex formation. We first demonstrate the existence of two distinct FACT-MCM subassemblies, FACT-MCM2/4/6/7 and FACT-MCM2/3/4/5. Both complexes possess DNA unwinding...

11p toshiba23 18-11-2011 45 2   Download