DNA recombination

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  • DNA replication, the process of copying one double stranded DNA molecule to produce two identical copies, is at the heart of cell proliferation. This book highlights new insights into the replication process in eukaryotes, from the assembly of pre-replication complex and features of DNA replication origins, through polymerization mechanisms, to propagation of epigenetic states. It also covers cell cycle control of replication initiation and includes the latest on mechanisms of replication in prokaryotes. The association between genome replication and transcription is also addressed.

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  • Chapter 6 - DNA structure, replication, and recombination. This chapter includes contents: Experimental evidence for DNA as the genetic material, the Watson and Crick double helix model of DNA, genetic information in DNA base sequence, DNA replication, recombination at the DNA level.

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  • Homologous recombination (HR) serves to eliminate deleterious lesions, such as double-stranded breaks and interstrand crosslinks, from chromosomes. HR is also critical for the preservation of replication forks, for telomere maintenance, and chromosome segregation in meiosis I. As such, HR is indispensable for the maintenance of genome integrity and the avoidance of cancers in humans. The HR reaction is mediated by a conserved class of enzymes termed recombinases.

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  • Since the discovery of the DNA structure researchers have been highly interested in the molecular basis of genome inheritance. This book covers a wide range of aspects and issues related to the field of DNA replication. The association between genome replication, repair and recombination is also addressed, as well as summaries of recent work of the replication cycles of prokaryotic and eukaryotic viruses. The reader will gain an overview of our current understanding of DNA replication and related cellular processes, and useful resources for further reading....

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  • Việc tinh chế enzyme cắt giới hạn đầu tiên (1970) và sử dụng nó để tạo ra các phân tử DNA tái tổ hợp đầu tiên trong ống nghiệm (1972-1973) là nền tảng cho sự ra đời của kỹ thuật di truyền (genetic engineering) và công nghệ DNA tái tổ hợp (recombinant DNA technology).

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  • Chương 2: CÔNG CỤ CHUYỂN GEN: PLASMID VÀ BACTERIOPHAGE Một phân tử DNA cần phải bộc lộ 1 vài đặc trưng tiêu biểu để có thể dùng như 1 công cụ để tạo dòng gen (gene cloning). Quan trọng nhất là chúng phải có khả năng tái bản trong tế bào chủ (host cell), tạo được 1 số lượng lớn các bản sao (copies ) của phân tử DNA tái tổ hợp (recombinant DNA molecule) và truyền được qua các tế bào con (daughter cells) . Một công cụ tạo dòng còn đòi hỏi phải có kích thước khá nhỏ, lý...

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  • Chương 6 Chöông 6: CLONING VECTOR CHO E.COLI Ngaøy nay, nhöõng thí nghieäm cô baûn lieân quan ñeán vieäc taïo cloning gene ñöôïc quan taâm.

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  • Bắt đầu từ những năm đầu thập niên 1970, các thành tựu về sinh học phân tử và kỹ thuật di truyền giúp chúng ta có thể hiểu rõ bản chất phân tử của các bệnh xuất hiện trên động vật, thực vật và đặc biệt là trên người. Giáo sư Paul Berg thuộc trường đại học Stanford (Hoa Kỳ), người được nhận giải Nobel hóa học năm 1980, là người đầu tiên tạo ra DNA tái tổ hợp (recombinant DNA- rDNA) vào năm 1972...

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  • Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E.

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  • Lập bản đồ gene của DNA ty thể * Lập bản đồ bộ gene ty thể của nấm men Có các phương pháp khác nhau xây dựng bản đồ bộ gene ty thể: - Lập bản đồ tái tổ hợp (recombination mapping): Ở nấm men sự phân li tế bào chất và tái tổ hợp xảy ra trong quá trình mọc chồi ở cytohet lưỡng bội. Sự phân li và tái tổ hợp có thể phát hiện trực tiếp ở các dạng lưỡng bội mọc chồi hay quan sát sản phẩm giảm phân khi chồi được kích thích tạo bào tử. Ví dụ...

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  • (BQ) Part 1 book "Principles and techniques of biochemistry and molecular biology" presents the following contents: Basic principles, cell culture techniques, centrifugation, microscopy, molecular biology, bioinformatics and basic techniques, recombinant DNA and genetic analysis, immunochemical techniques.

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  • Glofish is the first transgenic animal approved to be consumed by human in the USA  The insertion of different constructs of GFP into the fish genomes to give different green colors.The idea for recombinant DNA was first proposed by Peter Lobban, a graduate student of Prof. Dale Kaiser in the Biochemistry Department at Stanford University Medical School. The first publications describing the successful production and intracellular replication of recombinant DNA appeared in 1972 and 1973.

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  • Unequal Crossing-Over Normally, DNA recombination in germ cells occurs with remarkable fidelity to maintain the precise junction sites for the exchanged DNA sequences (Fig. 62-3). However, mispairing of homologous sequences leads to unequal crossover, with gene duplication on one of the chromosomes and gene deletion on the other chromosome. A significant fraction of growth hormone (GH) gene deletions, for example, involve unequal crossing-over (Chap. 333).

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  • Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Effects of recombinant human growth hormone on HIV-1-specific T-cell responses, thymic output and proviral DNA in patients on HAART: 48-week follow-up...

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  • Cells of the budding yeast, S. cerevisiae, have for several decades now been considered as the prototypic eukaryotic cells, ideally suited to study and uncover many of the basic phenomena of eukaryotic life.

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  • Rad51 and disrupted meiotic cDNA1 (Dmc1) are the two eukaryotic DNA recombinases that participate in homology search and strand exchange reactions during homologous recombination mediated DNA repair. Rad51 expresses in both mitotic and meiotic tissues whereas Dmc1 is confined to meiosis.

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  • DNA transactions in eukaryotes require that proteins gain access to target sequences packaged in chromatin. Further, interactions between distinct nucleoprotein complexes are often required to generate higher-order structures. Here, we employed two prokaryotic site-specific recombination systems to investigate how chromatin packaging affects the assembly of nucleoprotein structures of different complexities at more than 30 genomic loci. The dynamic nature of chromatin permitted protein–DNA and DNA–DNA interactions for sites of at least 34 bp in length.

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  • Activation-induced deaminase (AID) initiates switch recombination and somatic hypermutation of immunoglobulin genes in activated B cells. Compelling evidence now shows that AID travels with RNA polymerase II to deaminate actively transcribed DNA. reports deposited research A common mechanism for class-switch recombination and somatic hypermutation In all cells, high-fidelity pathways repair DNA to maintain the integrity of the genome. A handful

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  • The Mhr1 protein is necessary for mtDNA homologous recombination in Saccharomyces cerevisiae. Homologous pairing (HP) is an essential reaction during homologous recombination, and is generally catalyzed by the RecA⁄Rad51 family of proteins in an ATP-dependent manner. Mhr1 cata-lyzes HP through a mechanism similar, at the DNA level, to that of the RecA⁄Rad51 proteins, but without utilizing ATP.

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  • Meiotic recombination is initiated by DNA double-stranded breaks intro-duced by the SPO11 protein. Despite a decade of research, the biochemical functions of SPO11 remain largely unknown, perhaps because of difficulties in studying the functionally active SPO11.

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