English 8 unit 16

Five truncation mutants of chloroplast ATP synthasecsubunit from spin-ach (Spinacia oleracea) lacking 8, 12, 16, 20 or 60 N-terminal amino acids were generated by PCR by a mutagenesis method. The recombinantc genes were overexpressed inEscherichia coliand assembled withabsub-units into a native complex. The wild-type (WT)abcassembly i.e.abcWT exhibited high Mg 2+ -dependent and Ca 2+ -dependent ATP hydrolytic activity.

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