Eukaryotic homologous recombination

Rad51 and disrupted meiotic cDNA1 (Dmc1) are the two eukaryotic DNA recombinases that participate in homology search and strand exchange reactions during homologous recombination mediated DNA repair. Rad51 expresses in both mitotic and meiotic tissues whereas Dmc1 is confined to meiosis.

10p inspiron33 26-03-2013 54 5   Download

The human Rad51 protein, a eukaryotic ortholog of the bacterial RecA protein, is a key enzyme that functions in homologous recombination and recombinational repair of double strand breaks. The Rad51 protein con-tains two flexible loops, L1 and L2, which are proposed to be sites for DNA binding, based on a structural comparison with RecA.

12p inspiron33 25-03-2013 31 2   Download

DNA topoisomerase 6 (TOP6) belongs to a novel family of type II DNA topoisomerases present, other than in archaebacteria, only in plants. Here we report the isolation of full-length cDNAs encoding putative TOP6 sub-units A and B from rice (Oryza sativassp.indica), preserving all the struc-tural domains conserved among archaebacterial TOP6 homologs and eukaryotic meiotic recombination factor SPO11.

16p inspiron33 23-03-2013 41 4   Download

In eukaryotes, meiosis leads to genetically variable gametes through recom-bination between homologous chromosomes of maternal and paternal ori-gin. Chromatin organization following meiotic recombination is critical to ensure the correct segregation of homologous chromosomes into gametes. However, the mechanism of chromatin organization after meiotic recombi-nation is unknown.

10p media19 06-03-2013 43 2   Download