Express 4.0

Trong thế giới động vật, ong mật là loài thuộc lớp côn trùng. Chúng xuất hiện cách đây 40 triệu năm vào thời kỳ Eocene. Ong mật có ở khắp nơi trên thế giới gồm nhiều loài khác nhau. Mỗi loài có điều kiện môi trƣờng phát triển khác nhau và hiệu quả sản xuất cũng khác nhau. Sản phẩm đƣợc làm ra bởi ong mật nhƣ phấn hoa, mật ong, sáp ong, sữa ong chúa, keo ong, nọc ong là các loại thực phẩm dƣợc liệu độc đáo có giá trị kinh tế cao. Ngoài ra, ong mật còn...

94p canhchuon_1 21-06-2013 130 23   Download

The expression of mitochondrial and hydrogenosomal ADP/ATP carriers (AACs) from plants, rat and the anaerobic chytridiomycete fungus Neocallimastix spec. L2 in Escherichia coli allows a functional integration of the recombinant proteins into the bacterial cytoplasmic membrane. For AAC1 and AAC2 from rat, apparent Km values of about 40 lM for ADP, and 105 lM or 140 lM, respectively, for ATP have been determined, similar to the data reported for isolated rat mitochondria.

10p system191 01-06-2013 37 3   Download

The gene fprA of Mycobacterium tuberculosis, encoding a putative protein with 40% identity to mammalian adrenodoxin reductase, was expressed in Escherichia coli and the protein purified to homogeneity. The 50-kDa protein monomer contained one tightly bound FAD, whose fluorescence was fully quenched. FprA showed a low ferric reductase activity, whereas it was very active as a NAD(P)H diaphorase with dyes. Kinetic parameters were determined and the specificity constant (kcat/Km) for NADPH was two orders of magnitude larger than that of NADH....

9p system191 01-06-2013 36 3   Download

Retinoids are potent immune modulators that inhibit Fas ligand (FasL) expression and thereby repress the activationinduced apoptosis of immature thymocytes and T-cell hybridomas. In this study, we demonstrate that all-transretinoic acid (all-trans-RA) directly represses the transcriptional activity of the nuclear factors of activated T-cells (NFAT), which is an important transactivator of the FasL promoter.

9p research12 01-06-2013 42 5   Download

Arecombinant streptococcalC5apeptidasewas expressed in Escherichia coliand its catalytic properties and thermal sta-bility were subjected to examination. It was shown that the NH2 -terminal region ofC5a peptidase (Asn32–Asp79/ Lys90) forms the pro-sequence segment. Upon maturation the propeptide is hydrolyzed either via an autocatalytic intramolecular cleavage or by exogenous protease strepto-pain. At pH 7.4 the enzyme exhibited maximum activity in the narrow range oftemperatures between 40 and 43
C....

13p research12 29-04-2013 46 3   Download

To facilitate studies on di€erentiation of adrenocortical cells and regulation of steroidogenic genes, we established cell lines from adrenals of adult transgenic mice harboring a temperature-sensitive large T-antigen gene of simian virus 40. Adrenal glands of the mice exhibited normal cortical zonation including a functionally undi€erentiated cell-layer between the aldosterone-synthesizing zona glomerulosa cells and the corticosterone-synthesizing zona fasciculata cells.

13p research12 29-04-2013 44 2   Download

The gene encoding a subtilisin-like serine proteinase in the psychrotrophic Vibriosp. PA44 has been successfully cloned, sequenced and expressed in Escherichia coli.The gene is 1593 basepairs and encodes a precursor protein of 530 amino acid residues with a calculated molecular mass of 55.7 kDa. The enzyme is isolated, however, as an active 40.6-kDa proteinase, without a 139amino acid residue N-terminal prosequence.

11p tumor12 22-04-2013 51 2   Download

To assess the expression and physiological role of the mitochondrial NAD + -independent lactate dehydrogenase (iLDH) inEuglena gracilis, cells were grown with different carbon sources, and theD-andL-iLDHactivities and several key metabolic intermediates were examined. iLDH activity was significant throughout the growth period, increasing by three- to fourfold from latency to the stationary phase. Intracellular levels ofD-andL-lactate were high (5–40 mM) fromthe start of the culture and increased (20–80 mM)when the stationaryphasewas entered....

10p fptmusic 12-04-2013 37 1   Download

In the nematodeCaenorhabditis elegans, 40 genes code for astacin-likeproteins (nematodeastacins,NAS).The astacins are metalloproteases present in bacteria, invertebrates and vertebrates and serve avarietyof physiological functions like digestion, hatching, peptide processing, morphogenesis and pattern formation. With the exception of one distorted pseudogene, all the otherC. elegansastacins are expressed and are evidently functional. For 13 genes we found splicing patterns differing from the Genefinder predictions in WormBase, sometimes markedly....

12p fptmusic 12-04-2013 37 3   Download

The RNA recognition motif (RRM), also known as RNA-binding domain (RBD) or ribonucleoprotein domain (RNP) is one of the most abundant protein domains in eukaryotes. Based on the comparison of more than 40 structures including 15 complexes (RRM–RNA or RRM–protein), we reviewed the structure–function relationships of this domain. We identified and classified the different structural elements of the RRM that are import-ant for binding a multitude of RNA sequences and proteins.

14p awards 06-04-2013 40 2   Download

Cyclic AMP-dependent protein kinase (PKA) is a holoenzyme that consists of a regulatory (R) subunit dimer and two catalytic (C) subunits that are released upon stimulation by cAMP. Immunoblotting and immunoprecipita-tion of T-cell protein extracts, immunofluorescence of permeabilized T cells and RT⁄PCR of T-cell RNA using C subunit-specific primers revealed expression of two catalytically active PKA C subunits Ca1 (40 kDa) and Cb2 (47 kDa) in these cells.

0p awards 06-04-2013 37 3   Download

Thec-jun belongs to the family of proto-oncogenes and encodes for the protein Jun, a component of transcription factorAP-1 involved in regulation of the expression of genes indispensable for cell proliferation and differentiation. While the role ofc-junin the regulation of such genes has been well examined, the regulation ofc-junin proliferating cells is not fully understood.

11p awards 05-04-2013 31 3   Download

Approximately 400 million allergic patients are sensitized against group 1 grass pollen allergens, a family of highly cross-reactive allergens present in all grass species. We report the eukaryotic expression of the group 1 aller-gen from Timothy grass, Phl p 1, in baculovirus-infected insect cells. Domain elucidation by limited proteolysis and mass spectrometry of the purified recombinant glycoprotein indicates that the C-terminal 40% of Phl p 1, a major IgE-reactive segment, represents a stable domain....

0p awards 05-04-2013 27 2   Download

This report describes an efficient strategy for amplified functional purification of the human H1 receptor after heterologous expression in Sf9 cells. The cDNA encoding a C-terminally histidine-tagged (10xHis) human histamineH1 receptor was used to generate recombinant baculovirus in a Spodoptera frugiperda-derived cell line (IPLB-Sf9). As judged from its ligand affinity profile, functional receptor could be expressed at high levels (30–40 pmol per 10 6 cells).

11p dell39 03-04-2013 60 4   Download

The recent discovery of theNogo family ofmyelin inhibitors and theNogo-66 receptor opens upa very promising avenue for the development of therapeutic agents for treating spinal cord injury. Nogo-A, the largest member of the Nogo fam-ily, is a multidomain protein containing at least two regions responsible for inhibiting central nervous system (CNS) regeneration. So far, no structural information is available for Nogo-A or any of its structural domains. We have sub-cloned and expressed two Nogo-A fragments, namely the 182 residueNogo-A(567–748) and the 66 residueNogo-66 in Escherichia coli..

11p dell39 03-04-2013 48 4   Download

We report the development of a high-level bacterial expression system for the Alzheimer’s disease-associated amyloid b-peptide (Ab), together with a scaleable and inexpensive purification procedure. Ab(1–40) and Ab(1–42) coding sequences together with added ATG codons were cloned directly into a Pet vector to facilitate production of Met-Ab(1–40) and Met-Ab(1– 42), referred to as Ab(L1–40) and Ab(L1–42), respectively.

11p vinaphone15 27-02-2013 48 2   Download