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The interactions between photosystem I and five charge mutants of plastocyanin from the cyanobacteriumPhormi-dium laminosumwere investigatedin vitro. The dependence of the overall rate constant of reaction,k2, on ionic strength was investigated using laser flash photolysis. The rate con-stant of the wild-type reaction increased with ionic strength, indicating repulsion between the reaction partners. Remov-inganegativechargeonplastocyanin(D44A) acceleratedthe reactionandmade it independentof ionic strength; removing a positive charge adjacent to D44 (K53A) had little effect....

10p tumor12 22-04-2013 40 4   Download

The different effects of pH and NaCl on individual O2-binding properties ofaandbsubunits within liganded tetramer and dimer of human hemo-globin (HbA) were examined in a number of laser time-resolved spectro-scopic measurements. A previously proposed approach [Dzhagarov BM & Lepeshkevich SV (2004)Chem Phys Lett390, 59–64] was used to determine the extent of subunit dissociation rate constant difference and subunit affinity difference from a single flash photolysis experiment.

11p dell39 27-03-2013 48 4   Download