Generating a specific

Sweet sorghum is a new generation bio-energy crop with considerable tolerance to drought and salinity and amenable for multiple uses. Sweet sorghum has elicited the interest of breeder due to its capacity to provide renewable energy products, industrial commodities, food and animal feed. The sweet sorghum donor RSSV 313 is developed through a cross NSS223 x NARI 111 at MPKV, Rahuri through pedigree selection as identified and recommended as a donor parent in crop improvement programme based on specific physiological traits viz.

6p chauchaungayxua6 26-06-2020 10 2   Download

Prolyl endopeptidase (PEP) is a proline-specific oligopepti-dase with a reported effect on learning and memory in dif-ferent rat model systems. Using the astroglioma cell line U343,PEP expression was reduced by an antisense technique. Measuring different second-messenger concen-trations revealed an inverse correlation between inositol 1,4,5-triphosphate [Ins(1,4,5)P3] concentration and PEP expression in the generated antisense cell lines. However,no effect on cAMP generation was observed.

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To generate human antibodies against CXCR4, a seven-transmembrane chemokine receptor and a principal coreceptor forHIV-1, several roundsofPathfinder andStep-back selection from a large phage display antibody library were performed on Jurkat cells. A mAb against CXCR4 or biotinyated phage antibodies were used as guide molecules. Over 100 pan-Jurkat-cell-positive antibodies were charac-terized, but none were CXCR4 specific.

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This study describes the construction of a library of single-chain antibody fragments (scFvs) from a single human donor by individual amplification of all heavy and light variable domains (1.1·10 8 recombinants). The lib-rary was panned using the phage display technique, which allowed selection of specific scFvs (3F and C1) capable of recognizing Cn2, the major toxic component of Centruroides noxiusscorpion venom.

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Inactivation of factor Va (FVa) by activated protein C (APC) is a predominant mechanism in the down-regulation of thrombin generation. In normal FVa, APC-mediated inactivation occurs after cleavage at Arg306 (with corres-pondingrate constantk¢306) or after cleavage at Arg506 (k506 ) and subsequent cleavage at Arg306 (k306). We have studied the influence of heparin on APC-catalyzed FVa inactivation by kinetic analysis of the time courses of inac-tivation. Peptide bond cleavage was identified by Western blottingusingFV-specific antibodies. ...

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Understanding the sequence determinants of protein struc-ture, stabilityand folding is critical for understanding how natural proteins have evolved and how proteins can be engineeredtoperformnovel functions.Thecomplexityof the protein folding problem requires the abilityto search large volumes of sequence space for proteins with specific struc-tural or functional characteristics.

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Aloesone synthase (ALS) and chalcone synthase (CHS) are plant-specific type III poyketide synthases sharing 62% amino acid sequence identity. ALS selects acetyl-CoA as a starter and carries out six successive condensa-tions with malonyl-CoA to produce a heptaketide aloesone, whereas CHS catalyses condensations of 4-coumaroyl-CoA with three malonyl-CoAs to generate chalcone.

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Previous cross-linking studies [Kim E, Bobkova E, Hegyi G, Muhlrad A & Reisler E (2002)Biochemistry41, 86–93] have shown that site-specific cross-linking among F-actin monomers inhibits the motion and force generation of actomyosin. However, it does not change the steady-state ATPase parameters of actomyosin.

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Autodesk Robot™ Structural Analysis Professional software is a collaborative, versatile, and faster software application that can help you compete and win in the global economy. Purpose-built for BIM, Autodesk Robot Structural Analysis Professional calculates even your more complex models with powerful finite element auto-meshing, nonlinear algorithms, and a comprehensive collection of design codes to help you achieve results in minutes, not hours.

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Formylglycine-generating enzyme (FGE) catalyzes in newly synthesized sul-fatases the oxidation of a specific cysteine residue to formylglycine, which is the catalytic residue required for sulfate ester hydrolysis. This post-trans-lational modification occurs in the endoplasmic reticulum (ER), and is an essential step in the biogenesis of this enzyme family.

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Molybdenum insertion into the dithiolene group on the 6-alkyl side-chain of molybdopterin is a highly specific process that is catalysed by the MoeA and MogA proteins in Escherichia coli. Ligation of molybdate to molybdopterin generates the molybdenum cofactor, which can be inserted directly into molybdoenzymes binding the molybdopterin form of the molybdenum cofactor,

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Modern tools in proteomics require access to large arrays of specific bind-ers for use in multiplex array formats, such as microarrays, to decipher complex biological processes. Combinatorial protein libraries offer a solu-tion to the generation of collections of specific binders, but unit operations in the process to isolate binders from such libraries must be automatable to ensure an efficient procedure.

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We previously reported the use of a humanized bi-specific diabody that targets epidermal growth factor receptor and CD3 (hEx3-Db) for cancer immunotherapy. Bacterial expression can be used to express small recombi-nant antibodies on a large scale; however, their overexpression often results in the formation of insoluble aggregates, and in most cases artificial affinity peptide tags need to be fused to the antibodies for purification by affinity chromatography.

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There is an urgent need to develop new innovative therapies for the control of advanced cancer. The combination of antigen-specific immunotherapy with the employment of immunomodulatory agents has emerged as a potentially plausible approach for the control of advanced cancer. Methods: In the current study, we explored the combination of the DNA vaccine encoding calreticulin (CRT) linked to human papillomavirus type 16 (HPV-16) E7 antigen (CRT/E7) with the TLR7 agonist imiquimod for their ability to generate E7-specific immune responses and antitumor effects in tumor-bearing mice.

9p toshiba23 18-11-2011 59 5   Download