Genetic diversity analysis

Xem 1-20 trên 324 kết quả Genetic diversity analysis
  • Genetic diversity analysis of nine varieties of wheat (Triticum aestivum) was evaluated using 14 SSR markers. A genetic relationship was studied by calculating the genetic distances using an un-weighted pair-group method with arithmetic mean (UPGMA) subprogram of NTSYS-PC software. The cluster analysis shows that the most closely related varieties were V6 (GW1255) and V9 (GW366); V4 (GW11) and V8 (GW273), V1 (GW503) and V3 (GW451) respectively. V7 (GW173) and V3 (GW451) were the most distinct varieties among all the 9 varieties analyzed in this study.

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  • The present study was carried out at Dr R.H. Richharia Research Laboratory, Department of plant molecular biology and biotechnology, College of Agriculture Raipur (C.G.), Indira Gandhi Krishi Vishwavidyalaya, Raipur (Chhattisgarh) during Rabi 2013-14. Experimental materials comprised 24 Brassica compestris L. var. toria genotypes with the objectives to estimate the genetic diversity analysis at molecular level.

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  • A study was conducted between the 16 onion genotypes for genetic diversity analysis. The study involved the molecular techniques like RAPD ISSR and SSR markers. The markers showed genetic diversity remarkably. Among all markers ISSR and SSR markers gave diversified results than RAPD.

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  • The working group of G. hirsutum germplasm accessions was characterized for Distinctiveness, Uniformity and Stability testing. Subsequent analysis of data was done to study the genetic diversity available among the accessions using principal component and clustering of 320 cotton germplasm. Under field and laboratory, 26 qualitative traits and 14 quantitative traits were recorded. There is no variation observed for gossypol glands, anther filament colour, male sterility, boll bearing habit and boll opening.

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  • Daffodil (Narcissus spp.) belongs to family Amaryllidaceae and is a bulbous perennial grown for attractive flower, borne in spring sometimes autumn or winter. The present study was carried out to assess the genetic diversity present in daffodils in temperate region of Kashmir India using eleven sample sequence repeats (SSR’s). Twenty seven genotypes of daffodils belonging to different species such as Narcissus incomparabilis, Narcissus pseudonarcissus, Narcissus jonquilla, Narcissus poeticus, Narcissus papyraceus were evaluated for molecular characterization.

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  • Cytoplasmic Male sterility (CMS) is increasingly utilized for hybrid seed production of chilli (Capsicum annuum L.). Eight CMS and eight maintainer lines were studied for genetic diversity using ISSR markers. The ISSR analysis revealed substantial polymorphism in chilli. The results of the present study indicated the efficiency of ISSR markers in investigating genetic variability at molecular level, which is important for detecting variation of different genotypes of chilli and its utilization for further breeding programme.

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  • The present investigation to assess genetic diversity among 100 rice genotypes including five checks was conducted at Thyavanagi Village of Davanagere, Karnataka during Kharif 2016. Mahalanobis’ D2 statistics is used to quantify the degree of divergence. It is based on second degree statistics and pattern obtained by D2 does not change with number of characters. Mean values of 100 rice genotypes for ten characters were used to assess genetic distance between pair of genotypes. Genotypes were grouped into eleven clusters distribution patterns.

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  • Genetic divergence of 30 okra genotypes was studied using Mahalanobis D2 statistics revealed that considerable genetic diversity among genotypes in okra. Thirty diverse genotypes were grouped into six clusters with the highest of 11 genotypes in the cluster II, 10 in the cluster I, 4 genotypes in the cluster III, 3 in cluster V and 1 in each IV and VI cluster. The inter cluster distance was higher than the intra cluster distance indicating wide genetic diversity among the genotypes of different groups.

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  • Principal Component Analysis (PCA) is an important statistical tool through which we can easily access important polygenic characters which are of great importance in a plant breeding programme. The experiment was conducted with 35 germplasm accessions of brinjal to analyze genetic diversity at N. M. College of Agricultural, Navsari Agricultural University during the kharif season of 2016.

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  • Random amplified polymorphism DNA (RAPD) analysis was done to assess the diversity among 21 species/strains of Pleurotus. The morphologically similar species/ strains too gave a new account of the evolutionary process and taxonomy of mushrooms. A total of 150 10 mer primers were screened, out of which 10 primers viz. OPD-03, OPD-05, OPD08, OPA-13, OPA-16, OPQ-15, OPQ-16 and OPQ-18, S-1461 and S-1462 produced consistent polymorphic banding pattern.

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  • Cultivated sugarcane (Saccharum spp. complex, 2n = 80-120) belongs to the genus Saccharum of the family Poaceae. Sugarcane (Saccharum spp. complex) is an important cash crop of the tropical and subtropical regions of the world for its distinct characteristic of high sugar concentration accumulated in the stalk. Genetic diversity gives species the ability to adapt to changing environments, including new pests and diseases and new climatic conditions.

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  • Genetic diversity was assessed for 35 garcinia (Garcinia gummi-gutta (L) Roxb) germplasm maintained at Regional Agricultural Research Station, Kumarakom collected from different geographical locations using ISSR markers. Out of the 34 ISSR primers screened, 18 primers were polymorphic and used for further diversity analysis. The products were detected by electrophoresis and analysed using the program NTSYS-PC.

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  • Soybean is an important legume and oilseed crop with high protein (40%) and oil (20%). RAPD markers were used to access the genetic diversity among twenty four soybean genotypes. A total of Twenty primers were used out of which 18 got amplified which produced 164 bands and all were found polymorphic i.e. 100% polymorphism. The total number of amplified bands varied between 2 (OPF-19) and 16 (OPA-01) with an average of 9.1 bands per primer.

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  • Using molecular markers one can examine genetic diversity of species, cultivars, lines and forms of sunflower, identify genotypes, systematize collection material, classify heterosis groups and construct genetic maps. Fourteen parental genotypes of sunflower viz.,CMS-2A, CMS-821A, CMS-850A, R-10-46-2A, CMS-A4, CMS-A6, CMS-A10, R-GM-39, R-GM-41, R-GM-49, R-GM-69, 83-Br, R-393 and R2F01120B were analyzed for genetic diversity using 15 RAPD and 44 SSR primers. The genetic similarity index computed considering pooled data of SSR and RAPD markers showed very wide range from 0.18 to 0.

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  • The objective of this work was to evaluate the extent and distribution of genetic diversity among 25 accessions of Withania somnifera using RAPD markers and to determine the phylogenetic relationship amongst them with the future goal of assisting the marker assisted crop improvement programme.

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  • Random Amplified Polymorphic DNA (RAPD) markers were used to evaluate genetic similarity and interrelationship among 18 citrus cultivars, including 13 species and 5 hybrids. Out of 40 decamer primers screened, 25 were selected which produced 250 markers of which 231 were polymorphic and some species or cultivar specific RAPD markers.

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  • The present investigation was carried out with 32 diverse genotypes of bread wheat in completely randomized block design with 3 replications at Norman E. Borlaug Crop Research Centre, G.B. Pant University of agriculture & Technology Pantnagar for screening the genetic diversity for yield and physiological traits under normal sown condition. The observations were recorded on 16 agronomic traits and 3 physiological traits. The statistical analysis for genetic divergence was done using Mahalanobis-D 2 statistics and clustering of genotypes was done using Tocher method.

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  • Genetic diversity analysis was conducted at Main Agricultural Research Station, University of Agricultural Sciences, Dharwad during Kharif, 2017. In this study, 35 genotypes were evaluated for 11 yield and yield attributing traits and grouped into four clusters through Mahalanobis D2 statistic. Cluster I was the biggest with 23 genotypes followed by cluster II, III and IV. Among eleven characters studied, seed cotton yield (25.38 %) and days to 50 per cent flowering (22.86 %), contributed high for divergence while other characters like boll weight (0.34 %), number of sympodia per plant (0.

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  • Fifty-six germplasm lines of green gram [Vigna radiata (L.) Wilczek] were used to determine the extent of genetic diversity using Mahalanobis D2 statistic. The analysis was carried out for 20 quantitative traits. The D2 statistic performed on 20 quantitative traits grouped all 56 genotypes into eight divergent clusters. Cluster V (8559.766) had maximum intra-cluster distance while inter-cluster distance was highest between clusters III and VI(329997.

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  • Seed dormancy is an important trait affecting grain yield and quality in cereal crops. It is controlled by its own complicated genetic and environmental factors. The polymorphism pattern study of 24 rice genotypes for RM primers used in the present molecular study were clearly classified the rice genotypes into dormant (Cluster I, Cluster IIA) and nondormant (Cluster II B, Cluster III). In the present study, a total of 40 alleles were detected across the 24 rice genotypes by 11 polymorphic SSR markers. The number of alleles generated per locus by each marker ranged from 2 to 6.

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