Genotype methods

Xem 1-14 trên 14 kết quả Genotype methods
  • An ultimate goal of modern biology is to understand how the genetic blueprint of cells (genotype) determines the structure, function, and behavior of a living organism (phenotype). At the center of this scientific endeavor is characterizing the biochemical and cellular roles of proteins, the working molecules of the machinery of life. A key to understanding of functional proteins is the knowledge of their folded structures in a cell, as the structures provide the basis for studying proteins’ functions and functional mechanisms at the molecular level....

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  • In this study, we designed two PCR primers to amplify the region containing rs6548238. The AvaI restriction enzyme was selected to detect the SNP polymorphism. Sequencing the resulting bands validated the RFLP method. In conclusion, RFLP method is simple, sensitive and cost effective for genotyping of the TMEM18 rs6548238 polymorphism; thus, this method is most convenient for SNP analysis in large-size samples from Vietnamese population.

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  • The development and rapid implementation of molecular genotyping methods have revolutionized the possibility for differentiation and classification of microorganisms at the subspecies level. Investigation of the species diversity is required to determine molecular relatedness of isolates for epidemiological studies. Methods for molecular epidemiology of microorganisms must be highly reproducible and provide effective discrimination of epidemiologically unrelated strains.

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  • Over time, they have developed various characteristics to help them survive, which leads to plant diversity. It is essential to have regular assessments of the conservation status of all plant species, in order to prioritize those in need of conservation action and to provide a measure of the success of actions being taken. The improvement of cultivated plants considerably depends on the extent of genetic variability available within the species. Evaluation of different genotypes, varieties, population, etc.

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  • Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Clinical Microbiology đề tài: Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species...

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  • Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame

    pdf9p dauphong6 05-01-2012 32 3   Download

  • The birth of livestock genomics 15 years ago was inspired by the human genome initiative and the potential for capturing both its technologies and massive comparative data sets for application to livestock species, most of which are mammals. We are currently reaping the benefits of these efforts, with sequencing projects completed or ongoing in chickens, cattle, pigs, and horses and valuable mapping resources developed for others such as sheep and turkeys.

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  • Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame

    pdf9p dauphong8 14-01-2012 25 2   Download

  • Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: A comparison of alternative methods to compute conditional genotype probabilities for genetic evaluation with finite locus models

    pdf20p toshiba19 08-11-2011 25 1   Download

  • Yellow stem borer has been identified as a major insect pest of deepwater rice, causing severe yield losses. Bt gene(s) from Bacillus thuringiensis have been proven very effective in pest resistance program. The use of transgenic plants expressing Bt gene(s) is now occupied effective approach to control insect infestation. We have successfully introduced a synthetic cryIA(b) gene into embryogenic calli of a deepwater indica rice variety, Vaidehi, by using the biolistic method of transformation.

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  • The association of TGF b1 polymorphisms and atrial fibrillation (AF) in essential hypertensive (EH) subjects remains unknown. Methods EH subjects with AF (EH+AF+) and sinus rhythm (EH+AF-) were enrolled. The polymorphisms of +869 T ® C at codon 10 and + 915 G ® C at codon 25, were genotyped. The clinical characteristics including serum TGF b1 levels were detected. Results: The GG genotypes of TGF b1 +915 G ® C at codon 25 were more prevalent in subjects from EH+AF+ group than those from EH+AF- group (P = 0.009). The subjects with GG genotype from EH+AF+ group had the...

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  • Populations in Southeast AsiaandSouthChinahave highfrequencies ofa-thalassemia caused by a-globin gene mutations and/or deletions. This study was designed to find an efficient and simple diagnostic test for the mutations and deletions. A duplex polymerase chain reaction (PCR)/denaturing high-pressure liquid chromatography (DHPLC) was used to detect the mutations and deletions. A blinded study of 110 samples, which included 92 a-thalassemia samples with various genotypes and 18 normal DNA samples, was carried out by the methods.

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  • There is reason to expect strong genetic influences on the risk of developing active pulmonary tuberculosis (TB) among latently infected individuals. Many of the genome wide linkage and association studies (GWAS) to date have been conducted on African populations. In order to identify additional targets in genetically dissimilar populations, and to enhance our understanding of this disease, we performed a multi-stage GWAS in a Southeast Asian cohort from Indonesia. Methods: In stage 1, we used the Affymetrix 100 K SNP GeneChip marker set to genotype 259 Indonesian samples.

    pdf9p taisaocothedung 12-01-2013 18 1   Download

  • Objectives: To study prevalence of drug resistance and genotype testing for drug resistance HIV on HIV/AIDS patients with first-line antiretroviral treatment failure at Dongda Hospital, Hanoi. Subjects and methods: Cross-sectional, descriptive, prospective study on 47 HIV/AIDS patients with first-line antiretroviral treatment failure at Dongda Hospital, Hanoi during period from 6 - 2011 to 12 - 2016.

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