ISSR primers

Xem 1-20 trên 21 kết quả ISSR primers
  • Inter Simple Sequence Repeats, the PCR based technique was used to reveal the genetic diversity among wheat and its wild relatives. DNA profiling of 27 wheat genotypes at all the existing ploidy levels (diploid, tetraploid and hexaploid) was done using ISSR primers. All the primers were polymorphic. The primers resulted in the amplification of 73 bands out of which 17 bands were unique. Unique bands resulted in case of all the primers. According to these 4 ISSR primers genetic similarity value was observed ranging from 0 to 1, the highest genetic similarity value (1.

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  • Micropropagation is a highly sought after technique in the commercial production of orchid plants. This has the advantage of providing large number of plants in a short period of time. But a major constrain in the in vitro propagation technique is the somaclonal variation. It is important to produce true to type planting materials especially in case of the hybrids as they are more prone to variations. The true to type nature of the micropropagated plantlets can be confirmed by genetic fidelity analysis.

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  • Forty seven landraces of rice collected from different agro-climatic zones of Himachal Pradesh along with three check varieties were used in the present investigation for the diversity studies using molecular markers to know the their genetic relationship. Higher level of polymorphism was detected by both RAPD and ISSR analysis but the ISSR polymorphism percentage was higher than that of RAPD. Using fifteen RAPD and eleven ISSR primers, 81.2 and 86.4 percent of DNA polymorphism could be detected among these genotypes.

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  • Rice Stem borer is an important rice pest in India. In the present study, the genetic variability was analyzed among geographically isolated populations of rice yellow stem borer (YSB) from sixty locations of Odisha using ISSR marker technique. Fifty ISSR primers were screened and eleven ISSR primers were selected on the basis of clarity, usability, and reproducibility of their banding patterns. The 11 primers produced a total of 67 bright and discernible bands, all of which were polymorphic.

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  • The genetic fidelity of in vitro raised gerbera plants multiplied through micropropagation upto fifteen in vitro subcultures was assessed by using intersimple sequence repeat (ISSR) markers. 40 ISSR primers screened, 20 ISSR primers produced a total of 54 ISSRs clear, distinct and reproducible amplicons, which were monomorphic across all micropropagated plants (15) studied. Thus, a total 1094 bands were generated which exhibited homogeneous banding patterns with ISSR markers. All banding profiles from micropropagated plants were monomorphic and similar to those of the mother plant.

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  • Fusarium oxysporum f. sp. cumini is a serious disease of cumin (Cuminum cyminum L.). Ten isolates of F. oxysporum f. sp. cumini were isolated from major cumin growing districts of Gujarat state to compare molecular methods over traditional for differentiation among the isolates of pathogen. The average 98.70 % of polymorphism was recorded for all the 11 ISSR primers. The polymorphic information content (PIC) value ranged from 0.571 to 0.831 with an average of 0.739 per primer. The IISR primers grouped majority of the isolates based on their geographical location.

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  • Genetic diversity was assessed for 35 garcinia (Garcinia gummi-gutta (L) Roxb) germplasm maintained at Regional Agricultural Research Station, Kumarakom collected from different geographical locations using ISSR markers. Out of the 34 ISSR primers screened, 18 primers were polymorphic and used for further diversity analysis. The products were detected by electrophoresis and analysed using the program NTSYS-PC.

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  • Fenugreek [Trigonella foenum-graecum L.] is an annual herb of Leguminaceae family and is largely cultivated in warm temperate and tropical regions. The presence of variability in breeding population is prior need for crop improvement. This calls for an evaluation of genotypes to study the variability. The study was conducted to reveal the genetic diversity among 48 fenugreek genotypes which were collected from different geographical regions of India using Inter Simple Sequence Repeat markers.

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  • Random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers were used for comparative analysis of genetic variation in 42 sugar beet accessions. A total of 24 polymorphic primers (12 RAPD and 12 ISSR) were used.

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  • Phoenix dactylifera L. was introduced in Tunisia a very long time ago and plays an important socioeconomic role, especially in the south of the country. Genetic diversity and relationships among 26 cultivars were assessed with 7 ISSR primers.

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  • In view of the high level of morphological diversity in fern species, assessment of genetic variation among 19 fern species was evaluated using ten inter simple sequence repeat (ISSR) markers. The results suggested that the ISSR markers produced much better reproducible bands and were more efficient in grouping fern species. ISSR fragments generated 29 to 87 bands per primer. A total of 281 polymorphic bands generated 34.48 to 66.66 per cent polymorphism per primer. All germplasm were clearly differentiated by their ISSR fingerprints.

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  • Opium poppy (Papaver somniferum L.) is an important medicinal crop in the world. In the present investigation 24 prominent opium accessions were assessed by ISSR molecular markers for the genetic diversity analysis. A total of 116 amplified bands were obtained from the 15 primers, out of which 98 were polymorphic. Overall size of PCR amplified products had ranged between 300 bp to 2800 bp. The polymorphism percentage ranged from 16.66% to 100% for nine primers. The ISSR similarity matrix revealed the Jaccard’s similarity values lie between 0.35-0.90.

    pdf6p caygaocaolon3 09-03-2020 5 0   Download

  • Bananas (Musa spp.) are mostly diploid or triploid with various combinations of genomes inherited from their ancestors. Inter simple sequence repeat (ISSR) markers were used for evaluation of genetic diversity of some synthetic Banana Hybrids. A total of 10 primers were evaluated for banana hybrids based on ISSR polymorphism. Diversity analysis carried out by using ISSR markers in some Banana hybrids revealed the confirmation of the hybridization along with the phylogenetic relationship of the hybrids with their parents. Out of the 10 primers studied, six primers exhibited scorable markers.

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  • Cotton is an important fiber cash crop of India and cotton leaf curl disease is the major biotic constraint that can significantly reduce the production and productivity of the crop. Gossypium hirsutum L. suffered losses in Northern part of India mainly in Haryana due to high incidence of cotton leaf curl disease (CLCuD) and “whitefly” which is the vector of this disease. Development of resistant variety to this disease is most effective, long term and safe method to tackle with this problem.

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  • Genetic variability study of five isolates of Xanthomonas axonopodis pv. citri was carried out using molecular markers. The isolates were collected from five places in Maharashtra state, India. Morphological and Biochemical tests viz. gram’s reaction, starch hydrolysis, indole production, catalase test, KOH test, Gelatin liquefaction and Acid production test were carried out to identify and confirm the purity of the culture.

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  • Intra- and interspecific polymorphisms among fungal pathogens that cause wilt and root rot on chickpea were investigated by using 30 RAPD (random amplified polymorphic DNA) and 20 ISSR (inter-simple sequence repeats) primers. UPGMA (unweighted pair group method with arithmetic average) cluster analysis of RAPD and ISSR datasets using Dice’s coefficient differentiated all fungal isolates from each other and revealed considerable genetic variability between the isolates.

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  • Genetic diversity evaluation among 16 Gloriosa accessions collected from different locations in Tamil Nadu was studied by using Inter-simple sequence repeat (ISSR) markers. Thirty six ISSR primers were used, among that 16 primers showed 83.56 per cent polymorphism and produced 213 amplicons. This indicates that there is a high level of variation at the genetic level among these accessions. The primer UBC-807 showed the highest PIC value (0.958), which represented the high efficiency of the individual primer.

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  • Genetic uniformity of in vitro raised plants is a prerequisite for production of quality planting material of banana. Embryogenic cultures were induced from immature male flower buds of two important cultivars of banana, ‘Rasthali’ (Silk-Musa AAB) and ‘Grand Naine’ (Cavendish-Musa AAA). Cell suspensions were established and regenerated plants were evaluated for their genetic stability using 10 inter simple sequence repeat markers.

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  • The molecular evaluation of twelve chickpea (Cicer arietinum L.) varieties (screened for their resistance to fusarium wilt) at the Department of Plant Pathology and Agriculture Microbiology in 2014-15 was conducted to assess the genetic diversity and relationship of chickpea genotypes using RAPD. Twenty five primers of RAPD were used of which 15 primers gave amplification products. A total 349 amplicons were obtained of which 331 amplicons were polymorphic with 93.64 % level of polymorphism was observed.

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  • Present study was conducted on genetic diversity using ISSR markers for a total of 40 landraces of little millet (Panicum sumatrense) collected from five different districts of Madhya Pradesh. Ten ISSR markers amplified total 42 loci while 32 loci showed 76.19% polymorphism. Maximum number (06) of alleles were scored by the primers UBC-807 whereas, minimum number of alleles (03) were scored by the primers UBC-816. Percentage of the number of polymorphic loci within population among the three regions, the highest frequency of polymorphism was found in the Dindori region (97.

    pdf8p quenchua2 15-12-2019 13 0   Download



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