Lipid oxidation

In Vietnam, a tea-producing country, the tea buds and top three leaves are normally used for tea production while older leaves are pruned and discarded as agricultural waste in the winter. The present study aimed to exploit catechins from pruned tea leaves and use them as natural antioxidants for applications in the food industry.

12p vibecca 01-10-2024 4 2   Download

The study was carried out to investigate lipid and protein oxidation of herring (Sardinella gibbosa) that was treated with (2.5% w/v) sodium acetate at 4o C. After 3, 6, 9, and 12 days, lipid and protein oxidation were evaluated by indications such as pH value, peroxide (PV), thiobarbituric acid-reactive substances (TBARS), protease enzyme activity and sulfhydryl.

9p dianmotminh02 03-05-2024 3 1   Download

Bài giảng Hoá học hữu cơ 2 - Chương 27: Phân tử sinh học lipid, cung cấp cho người học những kiến thức như: Sáp, chất béo và dầu; phospholipid; xà phòng; prostaglandin và các eicosanoid khác; sinh tổng hợp steroid;...Mời các bạn cùng tham khảo!

62p khanhchi2530 04-05-2024 5 4   Download

Bài viết trình bày đánh giá sự biến đổi các chỉ số chống oxy hóa ở thủy thủ tàu ngầm trong quá trình an dưỡng. Phương pháp nghiên cứu: Mô tả cắt ngang tự đối chứng trên 60 thủy thủ thuộc Quân chủng Hải quân tham gia an dưỡng tại đơn vị X từ 7/2022 đến 7/2023. Đánh giá sự biến đổi các chỉ số chống oxy hóa của thủy thủ trước và sau an dưỡng, gồm: malondialdehyde (MDA) và trạng thái chống oxy hóa toàn phần (Total anti-oxidant status - TAS).

6p vipanda 29-01-2024 8 3   Download

A Total 8 semen sample from four healthy Gir breeding bulls were collected at weekly interval for two weeks period using of artificial vagina method for evaluation of semen biochemical attributes at various stages of cryopreservation of Gir bull semen. The overall mean values for oxidative stress parameters viz. lipid peroxidation and glutathione reductase were 51.14 ± 0.18 μmol/ml and 32.24 ± 0.64 U/L, respectively in neat semen samples.

7p nguaconbaynhay5 16-05-2020 16 0   Download

Part 1 Ensuring product safety, quality analysis part 2, part 3 Improving quality in the supply chain,... are the main contents of thein three parts Tài liệu Safety and quality issues in fish processing.Each reference peers

529p nhivo123 11-09-2015 87 8   Download

Những nhà nghiên cứu Đài loan đã thông báo trong trong tạp chí Journal of Hypertension tháng 12 năm 2004 sesamin có thể kiểm soát cải thiện huyết áp bởi nó kích thích tế bào sản xuất oxide nitric, từ đây có khả năng ức chế sự sản xuất ET-1 (ET-1 production) làm giảm huyết áp đối với bệnh cao huyết áp Sesamin còn làm giảm thấp mức lipid máu, còn có tác dụng như là chất chống oxy hóa, và có chức năng điều hòa kháng thể. ...

36p huyentrangho 31-05-2013 153 19   Download

Glyoxal is a key compound involved in glyoxal oxidase (GLOX)-dependent production of glyoxylate, oxalate and H2O2 by lignin-degrading basidiomycetes. In this paper, we report that glyoxal was produced from a metabolite of ligninolytic fungi, linoleic acid, by manganese peroxidase (MnP)-dependent lipid peroxidation. In the absence of the parent substrate of linoleic acid, the dialdehyde was oxidized by MnP and Mn(III) chelate to start free radical reactions with emission of chemiluminescence at 700– 710 nm. ...

9p system191 01-06-2013 69 7   Download

Glycosphingolipids (GSLs) were purified from adults and plerocercoids of the tapeworm Diphyllobothrium hottai, and their chemical structures were determined. Total lipid fractions prepared from chloroform/methanol extracts of whole tissues were fractionated successively on ion-exchange chromatography, silicic acid column chromatography, and preparative TLC. The purified GSLs were characterized by methylation analysis, TLC-immunostaining, liquid secondary ion MS, MALDI-TOF MS, and 1H-NMR.

11p research12 01-06-2013 38 4   Download

Lipoprotein lipase (LPL) plays a key role in lipid metabo-lism. Molecular modeling of dimeric LPL was carried out usingINSIGHT IIbased upon the crystal structures of human, porcine, and horse pancreatic lipase. The dimeric model reveals a saddle-shaped structure and the key heparin-binding residues in the amino-terminal domain located on the top of this saddle.

10p research12 29-04-2013 46 4   Download

Hexadecylphosphocholine (HePC) is a synthetic lipid rep-resentative of a new group of antiproliferative agents, alkylphosphocholines (APC), which are promising candi-dates in anticancer therapy. Thus we have studied the action of HePC on the human hepatoblastoma cell line HepG2, which is frequently used as a model for studies into hepatic lipid metabolism.

7p research12 23-04-2013 42 1   Download

We describe sensitive new approaches for detecting and quantitatingprotein–lipid interactionsusinganalytical ultra-centrifugation and continuous size-distribution analysis [Schuck (2000)Biophys. J.78,1606–1619]. The newmethods were developed to investigate the binding of human apo-lipoprotein E (apoE) isoforms to size-fractionated lipid emulsions, and demonstrate that apoE3 binds preferentially tosmall lipidemulsions,whereas apoE4exhibits apreference for large lipid particles.

11p tumor12 22-04-2013 33 2   Download

The oxidant function of pro-apoptotic protein Bax was investigated through heterologous expression in yeast. Direct measurements of fatty acid content show that Bax-expression induces oxidation of mitochondrial lipids. This effect is prevented by the coexpression of Bcl-xL. The oxi-dation actually could be followed on isolated mitochondria as respiration-induced peroxidation of polyunsaturatedcis-parinaric acid and on whole cells as the increase in the amount of thiobarbituric acid-reactiveproducts. ...

11p tumor12 22-04-2013 37 2   Download

The activity of m-calpain, a heterodimeric, Ca 2+ -dependent cysteine protease appears to be modulated by membrane interactions involving oblique-orientated a-helix formation by a segment, GTAMRILGGVI, in the protein’s smaller subunit. Here, graphical and hydrophobic moment-based analyses predicted that this segment may form ana-helix with strong structural resemblance to the influenza virus peptide, HA2, a known oblique-orientateda-helix former.

9p tumor12 22-04-2013 34 5   Download

Reactive oxygen species (ROS) are products of normal metabolic activities and are thought to be the cause of many diseases. A selenium-containing single-chain abzyme 2F3 (Se-2F3-scFv) that imitates glutathione peroxidase has been produced which has the capacity to remove ROS. To evaluate the antioxidant ability of Se-2F3-scFv, we con-structeda ferrous sulfate/ascorbate (Vc/Fe 2+ )-inducedmito-chondrial damage model system and investigated the capacity of Se-2F3-scFv to protect mitochondria from oxi-dative damage.

6p tumor12 20-04-2013 33 4   Download

Lactoferrin (LF) is a Fe 3+ -binding glycoprotein, first recognized in milk and then in other human epithelial secretions and barrier fluids. Many different functions have been attributed to LF, including protection from iron-induced lipid peroxidation, immunomodulation and cell growthregulation, DNA binding, and transcriptional acti-vation. Its physiological role is still unclear, but it has been suggested to be responsible for primary defense against microbial and viral infection.

9p tumor12 20-04-2013 36 3   Download

We have studied the interaction ofb-17, a potent synthetic antimicrobialb-peptide, with phospholipids. We find that unlikeother antimicrobial peptides suchasmagainin II,b-17 facilitates the formation of nonbilayer phases, indicating that the peptide promotes negative curvature. Studies of liposomal leakage also indicate a different mode of mem-brane interaction relative to magainin II, but both leakage andmembrane binding showthatb-17, likemagainin II, has strongaffinity formembranes containinganionic lipids....

9p tumor12 20-04-2013 47 2   Download

Ceramide is a lipid second messenger that acts on mul-tiple-target enzymes, some of which are involved in other signal-transduction systems. We have previously demon-strated that endogenous ceramide modifies the metabolism of brain ethanolamine plasmalogens. The mechanism involved was studied. On the basis of measurements of breakdown products, specific inhibitor effects, and previ-ous findings, we suggest that a plasmalogen-selective phospholipase A2 is the ceramide target.

11p tumor12 20-04-2013 33 3   Download

Specific lipids of the purple membrane ofHalobacteriaare required for normal bacteriorhodopsin structure, function, and photocycle kinetics [Hendler, R.W. & Dracheva, S. (2001) Biochemistry (Moscow)66, 1623–1627]. The decay of the M-fast intermediate through a path including the O intermediate requires the presence of a hydrophobic envi-ronment near four charged aspartic acid residues within the cytoplasmic loop region of the protein (R. W. Hendler & S. Bose, unpublished results).

6p fptmusic 16-04-2013 33 2   Download

Oxysterol binding protein (OSBP) and its homologs have been shown to regulate lipid metabolism and vesicular transport. However, the exact molecular function of individual OSBP homologs remains uncharacterized. Here we demonstrate that the yeast OSBP homolog, Osh6p, bound phos-phatidic acid and phosphoinositides via its N-terminal half containing the conserved OSBP-related domain (ORD). Using a green fluorescent protein fusion chimera, Osh6p was found to localize to the cytosol and patch-like or punctate structures in the vicinity of the plasma membrane....

13p fptmusic 12-04-2013 52 3   Download