Medicine of the future

The topology of central carbon metabolism ofAspergillus nigerwas identified and the metabolic network reconstruc-ted, by integrating genomic, biochemical and physiological information available for this microorganism and other related fungi. The reconstructed network may serve as a valuabledatabase for annotationof genes identified in future genome sequencing projects on aspergilli. Based on the metabolic reconstruction, a stoichiometric model was set up that includes 284metabolites and335reactions,...

11p tumor12 20-04-2013 26 3   Download

NAD(P)H regeneration is important for biocatalytic reactions that require these costly cofactors. A mutant phosphite dehydrogenase (PTDH-E175A⁄A176R) that utilizes both NAD and NADP efficiently is a very promising system for NAD(P)H regeneration. In this work, both the kin-etic mechanism and practical application of PTDH-E175A⁄A176R were investigated for better understanding of the enzyme and to provide a basis for future optimization.

12p fptmusic 11-04-2013 31 2   Download

Discovery-driven translational research in breast cancer is moving steadily from the study of cell lines to the analysis of clinically relevant samples that, together with the ever increasing number of novel and powerful tech-nologies available within genomics, proteomics and functional genomics, promise to have a major impact on the way breast cancer will be diag-nosed, treated and monitored in the future.

14p awards 05-04-2013 42 3   Download

The efficient engineering of enzymes with novel activities remains an ongoing challenge. Towards this end, genetic selection techniques provide a method for finding rare solutions to catalytic problems that requires only a limited foreknowledge of structure–function relationships. We have used genetic selections to extensively probe the structure and mechanism of chorismate mutases. The insights gained from these investigations will aid future enzyme design efforts.

8p dell39 03-04-2013 50 4   Download

Employing transgenic plants as alternative systems to the conventional Escherichia coli, Pichia pastorisor baculovirus hosts to produce recombin-ant allergens may offer the possibility of having available edible vaccines in the near future. In this study, two EF-hand-type Ca 2+ -binding allergens from olive pollen, Ole e 3 and Ole e 8, were produced in transgenic Arabid-opsis thaliana plants.

10p inspiron33 25-03-2013 40 3   Download

Elucidation of the involvement of protein kinase C subtypes in several dis-eases is an important challenge for the future development of new drug tar-gets. We previously identified the PKI55 protein, which acts as a protein kinase C modulator, establishing a feedback loop of inhibition.

9p media19 05-03-2013 28 1   Download

Understanding the structural basis of thermostability and thermoactivity, and their interdependence, is central to the successful future exploitation of extremophilic enzymes in biotechnology. However, the structural basis of thermostability is still not fully characterized.

11p media19 04-03-2013 25 4   Download