Modelling the bacterial
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Oligomerization into multimeric complexes is a prerequisite for the chaperone function of almost all a-crystallin type heat shock proteins (a-Hsp), but the molecular details of complex assembly are poorly understood. The a-Hsp proteins from Bradyrhizobium japonicum are suitable bacterial models for structure-function studies of these ubiquitous stress proteins. They fall into two distinct classes, A and B, display chaperone activity in vitro and form oligomers of 24 subunits.
9p 
research12
01-06-2013
49
6
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Human aromatase is responsible for estrogen biosynthesis and is implicated, in particular, in reproduction and estro-gen-dependent tumor proliferation. Themolecular structure model is largelyderived fromtheX-ray structure of bacterial cytochromes sharing only 15±20% identities with hP-450arom. In the present study, site directed mutagenesis experiments were performed to examine the role of K119, C124, I125, K130, E302, F320, D309, H475, D476, S470, I471 and I474 of aromatase in catalysis and for substrate binding....
13p research12
29-04-2013
33
3
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Ligand binding by the aryl hydrocarbon receptor (AhR), a member of the bHLH-PAS family of transcriptional reg-ulatory proteins, has been mapped to a region within the second ÔPASÕ domain, a conserved sequence motif ®rst discovered in the Per-ARNT-Sim family of proteins.
6p research12
29-04-2013
43
3
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Drosophilais a powerful model system to study the regula-tory and effector mechanisms of innate immunity. To iden-tify molecules induced in the course of viral infection in this insect, we have developed a model based on intrathoracic injection of the picorna-like DrosophilaCvirus(DCV).We have used MALDI-TOF mass spectrometry to compare the hemolymph of DCV infected flies and control flies. By contrast with the strong humoral response triggered by injectionof bacteriaor fungal spores, we have identifiedonly one molecule induced in the hemolymph of virus infected flies....
10p tumor12
20-04-2013
27
3
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Mitochondrial cytochromecoxidase is a key protonmotive component of the respiratory chain. Mutations in the mito-chondrially-encoded subunits of the complex have been reported in associationwith a range of diseases. In this work we used yeast and bacterial mutants to assess the effect of humanmutations insubunit 1 (L196I) andsubunit 3 (G78S, A200T,DF94–F98, F251L and W249Stop).
9p tumor12
20-04-2013
34
1
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Cold shock proteins (CSPs) form a family of highly conserved bacterial proteins capable of single-stranded nucleic acid binding. They are suggested to act as RNA chaperones during cold shock inhibiting the formation of RNA secondary structures, which are unfavourable for transcription and translation.
12p fptmusic
12-04-2013
37
2
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In this work we compared two plant ureases, jackbean urease (JBU) and embryo-specific soybean urease (SBU) and a bacterial (Bacillus pasteurii) urease, for kinetic parameters and other biological properties described recently for ureases that are independent of the ureolytic activity. The insecticidal effect of ureases was investigated in feedingtrials with the cotton sucker bug,Dysdercus peruvi-anus (Hemiptera) as an insect model. Contrastingwith B. pasteuriiurease (PBU), both plant ureases presented potent insecticidal activity, with LD50 values of 0.017% (w/w) and 0.
7p dell39
03-04-2013
43
2
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Kid, the toxin of theparD(kis, kid) maintenance system of plasmid R1, is an endoribonuclease that preferentially cleaves RNA at the 5¢ of A in the core sequence 5¢-UA(A⁄C)-3¢. A model of the Kid toxin interacting with the uncleavable mimetic 5¢-AdUACA-3¢ is available.
14p vinaphone15
25-02-2013
41
2
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Inclusion bodies are insoluble protein aggregates usually found in recombi-nant bacteria when they are forced to produce heterologous protein species. These particles are formed by polypeptides that cross-interact through sterospecific contacts and that are steadily deposited in either the cell’s cytoplasm or the periplasm.
9p cosis54
05-01-2013
43
4
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Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: The bacterial pathogen Listeria monocytogenes: an emerging model in prokaryotic transcriptomics...
4p toshiba4
07-09-2011
79
11
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