Monosaccharide glycosides

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  • In order to address the ever-increasing significance of carbohydrates in biology the presentation of material in this volume has been somewhat modified. A new introductory chapter (1) highlights some of the reviews published during the year that give an overview of the glycobiological aspects of carbohydrate chemistry, and another new chapter (20) brings together reports of the use of enzymic methods in the field - particularly those employed in the synthesis of glycosides and especially di- and oligosaccharides. There is consequently some inevitable overlap between...

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  • Review material published this year includes an essay ‘the unexpected and the unpredictable in organic synthesis’ which is a valuable account by Mukaiyama of the extraordinary history of his research, sections on stereoselective routes to sugars and glycosides being of special relevance.’

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  • - Polysaccharides are macromolecules, polymers with a few hundred to a few thousand monosaccharides joined by glycosidic linkages. - Some polysaccharides serve as storage material, hydrolyzed as needed to provide sugar for cells. - Other polysaccharides serve as building material for structures that protect the cell or the whole organism. - The architecture and function of a polysaccharide are determined by its sugar monomers and by the positions of its glycosidic linkages.

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  • A series of trehalose-based oligosaccharides were isolated from the cytoplasmic fraction of Mycobacterium smegmatis and purified by gel-filtration and paper chromatography and TLC. Their structures were determined by HPLC and GLC to determine sugar composition and ratios, MALDI-TOF MS to measure molecular mass, methylation analysis to determine linkages, 1H-NMR to obtain anomeric configurations of glycosidic linkages, and exoglycosidase digestions followed by TLC to determine sequences and anomeric configurations of the monosaccharides....

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