Number of genes

The development of fruits without fertilization is known as parthenocarpy. Parthenocarpy improves the fruit quality, processing attributes, production and productivity of vegetable crops like tomato, cucumber, watermelon etc. Absence of seeds can enhances the shelf life of the fruits, allowing a better conservation, fruit set in adverse climatic conditions, early and offseason production of vegetable crops. Therefore, it is important to ensure yield stability regardless of environmental conditions.

20p gaocaolon5 14-06-2020 16 3   Download

An optogenetic upgrade for the tet - OFF system fused the inhibitor to the blue light-responsive B-LID degron and optimized the performance of this construct with regard to the number of Med25VBD repeats. This approach resulted in an optogenetic upgrade of the popular Tet-OFF (TetR-VP64, tetO7-PhCMVmin)  system  that  allows tunable, blue light-inducible gene expression in HEK-293T  cells.

5p thuyancn 01-06-2015 30 5   Download

During the last decade several novel yeast genes encoding proteins related to the PPP family of Ser/Thr protein phosphatases have been discovered and their functional characterization initiated. Most of these novel phosphatases display intriguing structural features and/or are involved in a number of important functions, such as cell cycle regulation, protein synthesis and maintenance of cellular integrity. While in some cases these genes appear to be restricted to fungi, in others similar proteins can be found in higher eukaryotes. ...

6p research12 01-06-2013 27 3   Download

The cAMP-dependent protein kinase (PKA) signaling pathway plays a major role in a number of pathophysio-logical conditions. However, there have been conflicting evidences regarding the action of cAMP/PKA on nuclear factor-jB(NF-jB). Inthis study,wehaveexplored the effect of cAMP/PKA on NF-jBactivity and determined its molecular mechanism. PKA activating agents or expression of thecatalyticsubunitofPKA(PKAc) inhibitedtheNF-jB-dependent reporter gene expression induced by tumor nec-rosis factora(TNFa). ...

7p research12 23-04-2013 33 4   Download

Imidazolium trans-imidazoledimethyl sulfoxide-tetrachlo-roruthenate (NAMI-A) is a novel ruthenium-containing experimental antimetastatic agent. Compelling evidence ascribes apivotal role toendothelial cells in theorchestration of tumor angiogenesis and metastatic growth, suggesting antiangiogenic therapy as an attractive approach for anticancer treatment.

10p tumor12 22-04-2013 42 2   Download

Multi-protein complexes are emerging as important entities of biological activity inside cells that serve to create func-tional diversity by contextual combination of gene products and, at the same time, organize the large number of different proteins into functional units. Many a time, when studying protein complexes rather than individual proteins, the bio-logical insight gained has been fundamental, particularly in cases in which proteins with no previous functional anno-tation couldbe placed intoa functional context derived from their
molecular environment. ...

9p tumor12 20-04-2013 38 2   Download

Induction of CYP3A genes by the ligand-activated preg-nane-X-receptor (PXR)involves the interaction of other as yet unidentified liver transcription factors. Here we show that the CYP3A1promoter contains two active sites con-trolled by the CCAAT/enhancer-binding protein a (C/ EBPa), previously shown to regulate a number of liver stress response genes. We have identified two functional C/EBP binding sites at theCYP3A1promoter that confer luciferase activity to C/EBPacotransfected CHO cells.

9p tumor12 20-04-2013 43 4   Download

The c-jungene encodes the protein Jun, a component of the essential transcription factor, AP1. Jun/AP-1 occupies a central position in signal transduction pathways as it is responsible for the induction of a number of genes in response to growth promoters. However, the exact mecha-nisms leading to an enhanced expression of the c-jun gene itself during proliferation, differentiation, cell growth and development are not fully understood. Cell culture studies have given some insight in the mechanisms involved in the up-regulation of c-jun expression by UV irradiation and phorbol esters. ...

9p tumor12 20-04-2013 43 2   Download

The transcription factorNF-jB is activated in cellular stress responses. This requires rapid regulation of its function, which is accomplished, in part, by various modes of phos-phorylation. Even though diverseDNAbinding subunits of NF-jB proteins may transactivate fromdistinct recognition sequences, the differential regulation of transcription from the large number of NF-jB responsive sites in various gene promoters andenhancershasbeen incompletelyunderstood.

12p fptmusic 16-04-2013 52 3   Download

The wealth of available genomic data has spawned a corresponding interest in computational methods that can impart biological meaning and context to these experiments. Traditional computational methods have drawn rela-tionships between pairs of proteins or genes based on notions of equality or similarity between their patterns of occurrence or behavior. For exam-ple, two genes displaying similar variation in expression, over a number of experiments, may be predicted to be functionally related.

9p fptmusic 12-04-2013 41 4   Download

Plasminogen activator inhibitor type-2 (PAI-2) is a nonconventional serine protease inhibitor (serpin) with unique and tantalizing properties that is generally considered to be an authentic and physiological inhibitor of uro-kinase. However, the fact that only a small percentage of PAI-2 is secreted has been a long-standing argument for alternative roles for this serpin.

10p fptmusic 12-04-2013 38 2   Download

In eukaryotes, pre-rRNA processing depends on a large number of non-ribosomal trans-acting factors that form large and intriguingly organized complexes. A novel nucleolar protein, Nop53p, was isolated by using Nop17p as bait in the yeast two-hybrid system. Nop53p also interacts with a second nucleolar protein, Nip7p. A carbon source-conditional strain with the NOP53coding sequence under the control of theGAL1promoter did not grow in glucose-containing medium, showing the phenotype of an essential gene. ...

14p fptmusic 11-04-2013 32 2   Download

In the majority of hypophosphatasia patients, reductions in the serum lev-els of alkaline phosphatase activity are caused by various missense muta-tions in the tissue-nonspecific alkaline phosphatase (TNSALP) gene. A unique frame-shift mutation due to a deletion of T at cDNA number 1559 [TNSALP (1559delT)] has been reported only in Japanese patients with high allele frequency.

14p awards 06-04-2013 43 4   Download

The Mycobacterium tuberculosisgenome contains an unusually high number of proteins involved in the metabo-lism of lipids belonging to the Lip family, including various nonlipolytic and lipolytic hydrolases. Driven by a structural genomic approach, we have biochemically characterized the Rv1399c gene product, LipH, previously annotated as a putative lipase. Rv1399c was overexpressed inE. colias inclusion bodies and refolded. Rv1399c efficiently hydro-lyzes soluble triacylglycerols and vinyl esters.

0p awards 05-04-2013 49 3   Download

The dementia familial encephalopathy with neuroserpin inclusion bodies (FENIB) is caused by point mutations in the neuroserpin gene.We have shown a correlation between the predicted effect of the mutation and the number of intracerebral inclusions, and an inverse relationshipwith the age of onset of disease. Our previous work has shown that the intraneuronal inclusions in FENIB result from the sequential interactionbetween the reactive centre loopofone neuroserpin molecule withb-sheet A of the next. ...

0p awards 05-04-2013 31 3   Download

The hypothesis thatL-glutamate (Glu) is an excitatory amino acid neurotransmitter in the mammalian central nervous system is now gaining more support after the suc-cessful cloning of a number of genes coding for the signaling machinery required for this neurocrine at synapses in the brain. These include Glu receptors (signal detection), Glu transporters (signal termination) and vesicular Glu trans-porters (signal output through exocytotic release).

13p dell39 03-04-2013 64 4   Download

The sequencing of the genome ofRalstonia solanacearum[Salanoubat M, Genin S, Artiguenave F,et al.(2002) Nature415, 497–502] revealed several genes that putatively code for polyphenol oxidases (PPOs). This soil-borne pathogenic bacterium withers a wide range of plants. We detected the expression of two PPO genes (accession numbers NP_518458 and NP_519622) with high similarity to tyrosinases, both containing the six conserved histidines required to bind the pair of type-3 copper ions at the active site....

14p dell39 27-03-2013 47 3   Download

Group I intron ribozymes require cations for folding and catalysis, and the current literature indicates that a number of cations can promote folding, but only Mg 2+ and Mn 2+ support both processes. However, some group I introns are active only with Mg 2+ , e.g. three of the five group I introns in Chlamydomonas reinhardtii. We have investigated one of these ribozymes, an intron from the 23S LSU rRNA gene of Chlamydomonas reinhardtii (Cr.LSU), by determining if the inhibition by Mn 2+ involves catalysis, folding, or both....

14p inspiron33 26-03-2013 46 3   Download

Catharanthus roseus(L.) G. Don produces a number of biologically active terpenoid indole alkaloids via a complex terpenoid indole alkaloid biosyn-thetic pathway. The final dimerization step of this pathway, leading to the synthesis of a dimeric alkaloid, vinblastine, was demonstrated to be cata-lyzed by a basic peroxidase.

14p galaxyss3 21-03-2013 60 5   Download

The cAMP response element-binding protein (CREB) was identified almost 20 years ago. Since then, CREB has become one of the most extensively studied tran-scription factors. Currently, the importance of CREB for numerous physiological events has been confirmed by the number of targets: up to 4000 genes or up to 6000 loci.

1p galaxyss3 19-03-2013 34 4   Download