Patterns for reporting

The broad objective of research reported here was to develop a multi-publishing workflow for student atlases.

162p runthenight05 01-03-2023 5 3   Download

This thesis reports research on analysis of the variance of surface electromyogram (sEMG) for healthy participants and people suffering with Lower Back Pain (LBP) when they are walking and running. SEMG signal recorded when the participants were walking and running on a treadmill. The strength and duration of the muscle activity for each heel strike were the features.

129p runthenight07 01-03-2023 10 3   Download

Cloning and over-expression of human glucose 6-phosphate dehydrogenase (Glc6P dehydrogenase) has for the first time allowed a detailed kinetic study of a preparation that is genetically homogeneous and in which all the protein molecules are of identical age. The steady-state kinetics of the recombinant enzyme, studied by fluorimetric initial-rate measurements, gave converging linear Lineweaver–Burk plots as expected for a ternary-complex mechanism.

8p research12 01-06-2013 59 4   Download

The centromere–kinetochore complex is a highly specialized chromatin domain that both mediates and monitors chromosome–spindle interactions responsible for accurate partitioning of sister chromatids to daughter cells. Centromeres are distinguished from adjacent chromatin by specific patterns of histone modification and the presence of a centromere-specific histone H3 variant (e.g. CENP-A). Centromere-proximal regions usually correspond to sites of avid and persistent sister chromatid cohesion mediated by the conserved cohesin complex.

15p research12 01-06-2013 49 4   Download

From the antennae of the moth Mamestra brassicae, we have identified a lepidopteran G protein a subunit belonging to the Gq family, through immunological detection in crude antennal extract and antennal primary cell cultures, followed by molecular cloning. The complete cDNA sequence (1540 bp) contains an open reading frame encoding a protein of 353 amino acids.

10p research12 01-06-2013 26 4   Download

A technique has been developed to search a proteome database for new members of a functional class of membrane protein. It takes advantage of the highly conserved secondary structure of functionally related membrane proteins. Such proteins typically have the same number of transmembrane domains located at similar relative positions in their polypeptide sequence. This gives rise to a characteristic pattern of peaks in their hydropathy profiles.

7p research12 01-06-2013 32 4   Download

Decorin is a small leucine-rich chondroitin/dermatan sulfate proteoglycan reported to interact with ®brillar collagens through its protein core and to localize atdandebands of the collagen ®bril banding pattern. Using a solid-phase assay, we have determined the interaction of peptides derivedbyCNBr cleavage of type I and type II collagenwith decorin extracted from bovine tendon and its protein core and with a recombinant decorin preparation.

10p research12 29-04-2013 36 3   Download

Fourier transform infrared spectroscopy was performed on human leukemic daunorubicin-sensitiveK562 cells and their multiresistant counterpart derived by selection. Statistical analysis, including variable reduction and linear discrimi-nant analysis was performed on sensitive and multiresistant cells spectra in order to establish a diagnostic tool for multiresistant pattern. For each of the twomethods of data reduction tested [genetic algorithm or principal component analysis (PCA)] discriminationbetween the twocell lineswas found to be possible....

6p research12 23-04-2013 43 2   Download

The cystic ®brosis transmembrane conductance regulator (CFTR) gene shows a complex pattern of expression, with temporal and spatial regulation that is not accounted for by elements in the promoter. One approach to identifying the regulatory elements forCFTRis the mapping of DNase I hypersensitive sites (DHS) within the locus. We previously identi®ed at least 12 clusters of DHS across theCFTRgene andhere further evaluateDHS in introns 2, 3, 10, 16, 17a, 18, 20 and 21 to assess their functional importance in regulation ofCFTRgene expression. ...

7p research12 23-04-2013 30 2   Download

E2F transcription factors play an important role in the regulation of cell cycle progression. E2F6, the most recently identified member of the E2F family, is a retinoblastoma-protein-independent transcriptional repressor that is required for developmental patterning of the axial skeleton. It has recently been shown that theE2f6locus produces two different mRNAs, E2F6 and E2F6b. The E2F6b mRNA contains an additional exon that is inserted by alternative splicing.

7p tumor12 22-04-2013 32 1   Download

Limitedproteolysis of the pyruvate decarboxylase (E1,a2b2 ) component of the pyruvate dehydrogenase (PDH) multi-enzyme complexofBacillus stearothermophilushas indicated the importance for catalysis of a site (Tyr281-Arg282) in the E1asubunit (Chauhan, H.J., Domingo, G.J., Jung, H.-I. & Perham, R.N. (2000)Eur. J. Biochem.267, 7158–7169). This site appears to be conserved in the a-subunit of hetero-tetrameric E1s and multiple sequence alignments suggest that there are additional conserved amino-acid residues in this region, part of a common pattern with the consensus sequence -YR-H-D-YR-DE-.

10p tumor12 20-04-2013 54 3   Download

HIV-1 protease is an important target for treatment of AIDS, and efficient drugs have been developed. However, the resistance and negative side effects of the current drugs has necessitated the development of new compounds with different binding patterns. In this study, nine C-terminally duplicated HIV-1 protease inhibitors were cocrystallised with the enzyme, the crystal structures analysed at 1.8–2.3 A˚ resolution, and the inhibitory activity of the compounds characterized inorder toevaluate the effects of the individual modifications. ...

13p fptmusic 16-04-2013 44 3   Download

The wealth of available genomic data has spawned a corresponding interest in computational methods that can impart biological meaning and context to these experiments. Traditional computational methods have drawn rela-tionships between pairs of proteins or genes based on notions of equality or similarity between their patterns of occurrence or behavior. For exam-ple, two genes displaying similar variation in expression, over a number of experiments, may be predicted to be functionally related.

9p fptmusic 12-04-2013 41 4   Download

Serine⁄threonine kinase 33 (STK33⁄Stk33) is a recently discovered gene whose inferred amino acid sequence translation displays characters typical for a calcium⁄calmodulin dependent kinase (CAMK). In this study we ana-lysed the STK33⁄Stk33RNA and protein distribution and the localization of the protein. TheSTK33⁄Stk33expression pattern resembles those of some related members of the CAMK group.STK33⁄Stk33displays a non-ubiquitous and, in most tissues, low level of expression.

15p fptmusic 12-04-2013 38 2   Download

In the nematodeCaenorhabditis elegans, 40 genes code for astacin-likeproteins (nematodeastacins,NAS).The astacins are metalloproteases present in bacteria, invertebrates and vertebrates and serve avarietyof physiological functions like digestion, hatching, peptide processing, morphogenesis and pattern formation. With the exception of one distorted pseudogene, all the otherC. elegansastacins are expressed and are evidently functional. For 13 genes we found splicing patterns differing from the Genefinder predictions in WormBase, sometimes markedly....

12p fptmusic 12-04-2013 37 3   Download

Photodynamic treatment (PDT) is an emerging therapeutic procedure for the management of cancer, based on the use of photosensitizers, compounds that generate highly reactive oxygen species (ROS) on irradiation with visible light. The ROS generated may oxidize a variety of bio-molecules within the cell, loaded with a photosensitizer. The high reactivity of these ROS restricts their radius of action to 5–20 nm from the site of their generation.

7p fptmusic 12-04-2013 44 3   Download

The major 2S albumin allergen from Brazil nuts, Ber e 1, was subjected to gastrointestinal digestion using a physiologically relevantin vitro model sys-tem either before or after heating (100
C for 20 min). Whilst the albumin was cleaved into peptides, these were held together in a much larger struc-ture even when digested by using a simulated phase 1 (gastric) followed by a phase 2 (duodenal) digestion system. Neither prior heating of Ber e 1 nor the presence of the physiological surfactant phosphatidylcholine affected the pattern of proteolysis.

0p awards 05-04-2013 41 2   Download

HIV-1 protease is a pivotal enzyme in the later stages of the viral life cycle which is responsible for the processing and maturation of the virus particle into an infectious virion. As such,HIV-1proteasehasbecomean important target for the treatment ofAIDS, and efficient drugs have beendeveloped. However, negative side effects and fast emerging resistance to the current drugs have necessitated the development of novel chemical entities in order to exploit different phar-macokinetic properties as well as new interaction patterns....

9p awards 05-04-2013 32 2   Download

The LmbB1 protein, participating in the biosynthesis of lincomycin,was heterologously expressed inEscherichia coli, purified in its active form, and characterized as a dimer of identical subunits. Methods for purification and analysis of the LmbB1 reaction product were developed. Molecular mass and fragmentation pattern of the product revealed by capillary electrophoresis-mass spectrometry were in agree-ment with its proposed structure, 4-(3-carboxy-3-oxo-propenyl)-2,3-dihydro-1H-pyrrole-2-carboxylic acid.

6p awards 05-04-2013 36 5   Download

To test thioredoxin resistance to oxidizing free radicals, we have studied the one-electron oxidation of wild-type thio-redoxin and of two forms with the point mutations D30A and W35A, using azide radicals generated byc-ray or pulse radiolysis. The oxidation patterns of wild-type thioredoxin and D30A are similar. In these forms, Trp35 is the primary target and is
repaired by one-electron reduction; first by intramolecular electron transfer from tyrosine, and then fromother residues. Conversely, duringoxidationofW35A, Trp13 is poorly reactive.

7p awards 05-04-2013 37 4   Download