Protein – examples from

Shikimate kinase was chosen as a convenient representative example of the subclass of a/b proteins with which to examine the mechanism of protein folding. In this paper we report on the refolding of the enzyme after denaturation in urea. As shown by the changes in secondary and tertiary structure monitored by far UV circular dichroism (CD) and fluorescence, respectively, the enzyme was fully unfolded in 4 M urea. From an analysis of the unfolding curve in terms of the two-state model, the stability of the folded state could be estimated as 17 kJÆmol)1.

9p system191 01-06-2013 46 5   Download

14-3-3 proteins play an important role in a multitude of signalling pathways. The interactions between 14-3-3 and other signalling proteins, such as Raf and KSR (kinase suppressor of Ras), occur in a phospho-specific manner. Recently, a phosphorylation-independent interaction has been reported to occur between 14-3-3 and several proteins, for example 5-phosphatase, p75NTR-associated cell death executor (NADE) and the bacterial toxin Exoenzyme S (ExoS), an ADP-ribosyltransferase from Pseudomonas aeruginosa. ...

9p tumor12 22-04-2013 25 3   Download

Most of the examples of protein translocation across a membrane (such as the import of classical secretory proteins into the endoplasmic reticulum, import of proteins into mitochondria and peroxisomes, as well as protein import into and export from the nucleus), are understood in great detail.

11p fptmusic 16-04-2013 39 4   Download

We have studied the reaction native«denatured for the 33-kDa protein isolated from photosystem II. Sucrose and glycerol have profound effects on pressure-induced unfolding. The additives shift the equilibrium to the left; they also cause a significant decrease in the standard volume change (DV). The change in DVwas related to the sucrose and glycerol concentrations.

8p fptmusic 16-04-2013 59 5   Download

A correct three-dimensional structure is a prerequisite for protein function-ality, and therefore for life. Thus, it is not surprising that our cells are packed with proteins that assist protein folding, the process in which the native three-dimensional structure is formed.

28p galaxyss3 07-03-2013 37 5   Download

The 21st century is bringing a flood of genomic sequence data from diverse species and although this information is highly accessible, we are far from understanding its content. Recent discoveries, for example on novel RNA types, clearly show that there is still much to learn.

1p media19 05-03-2013 28 3   Download

The 1,3(4)-b-d-glucanases of glycoside hydrolase family 16 provide useful examples of versatile yet specific protein–carbohydrate interactions. In the present study, we report the X-ray structures of the 1,3(4)-b-d-glucanase Phanerochaete chrysosporiumLaminarinase 16A in complex withb-glucan products from laminarin (1.6 A˚ ) and lichenin (1.1 A ˚ ) hydrolysis. The G6G3G3G glucan, in complex with the enzyme, showed ab-1,6 branch in the acceptor site.

12p vinaphone15 25-02-2013 41 2   Download

There is widespread acceptance that cationic antimicrobial peptides, apart from their membrane-permeabilizing⁄disrupting properties, also operate through interactions with intracellular targets, or disruption of key cellu-lar processes. Examples of intracellular activity include inhibition of DNA and protein synthesis, inhibition of chaperone-assisted protein folding and enzymatic activity

14p viettel02 20-02-2013 30 4   Download

Functional protein serotonylation is a newly recognized post-translational modification with the primary biogenic monoamine (PBMA) serotonin (5-HT). This covalent protein modification is catalyzed by transglutaminas-es (TGs) and, for example, acts in the constitutive activation of small GTP-ases.

16p cosis54 08-12-2012 38 3   Download