Realistic case studies

Much of enzyme kinetics builds on simplifications enabled by the quasi-steady-state approximation and is highly useful when the concentration of the enzyme is much lower than that of its substrate. However, in vivo, this condition is often violated. In the present study, we show that, under con-ditions of realistic yet high enzyme concentrations, the quasi-steady-state approximation may readily be off by more than a factor of four when pre-dicting concentrations.

16p viettel02 20-02-2013 32 2   Download