Reexamining

In the O2– generating flavocytochrome b, the membranebound component of the neutrophil NADPH oxidase, electrons are transported from NADPH to O2 in the following sequence: NADPH fi FAD fi heme b fi O2. Although p-iodonitrotetrazolium (INT) has frequently been used as a probe of the diaphorase activity of the neutrophil flavocytochrome b, the propensity of its radical to interact reversibly with O2 led us to question its specificity. This study was undertaken to reexamine the interaction of INT with the redox components of the neutrophil flavocytochrome b. ...

10p research12 01-06-2013 42 3   Download

UMP-CMP kinase catalyses an important step in the phosphorylationofUTP, CTPanddCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies. The reactivity of human UMP-CMP kinase towards natural substrates and nucleotide analogs was reexamined. The expression of the recombinant enzyme and conditions for stability of the enzyme were improved. Substrate inhibition was observed for UMP and CMP at concentrations higher than 0.2 mM, but not for dCMP.

7p fptmusic 16-04-2013 37 3   Download