Streptomyces coelicolor

Polyhydroxyalkanoate (PHA) is stored as an important carbon and energy source in bacterial cells. For biomedical applications, gram-positive bacteria can be better sources of PHAs, since they lack outer membrane lipopolysaccharide. Although gram-positive Streptomyces coelicolor A3(2) has been indicated as a high potential PHA producer, phaC gene that encodes the key enzyme PHA synthase in the metabolic pathway is not determined in its genome.

12p thiencuuchu 27-11-2021 18 2   Download

We have investigated the crr gene of Streptomyces coelicolor that encodes a homologue of enzyme IIAGlucose of Escherichia coli, which, as a component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) plays a key role in carbon regulation by triggering glucose transport, carbon catabolite repression, and inducer exclusion. As in E. coli, the crr gene of S. coelicolor is genetically associated with the ptsI gene that encodes the general phosphotransferase enzyme I.

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The folding of a 93-residue protein, the histidine-phospho-carrier protein ofStreptomyces coelicolor,HPr,hasbeen studied using several biophysical techniques, namely fluo-rescence, 8-anilinonaphthalene-1-sulfate binding, circular dichroism, Fourier transform infrared spectroscopy, gel filtration chromatography and differential scanning calori-metry. The chemical-denaturation behaviour of HPr, fol-lowed by fluorescence, CD and gel filtration, at pH 7.5 and 25
C, is described as a two-state process, which does not involve the accumulation of thermodynamically stable intermediates.

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TheSCO2299gene fromStreptomyces coelicolorencodes a single peptide consisting of 497 amino acid residues. Its N-terminal region shows high amino acid sequence similarity to RNase HI, whereas its C-terminal region bears similarity to the CobC protein, which is involved in the synthesis of cobalamin. The SCO2299gene suppressed a temperature-sensitive growth defect of anEscherichia coliRNase H-deficient strain, and the recombinant SCO2299 protein cleaved an RNA strand of RNAÆDNA hybridin vitro.

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In Gram-positive bacteria, the expression of iron-regulated genes is medi-ated by a class of divalent metal-dependent regulatory (DmdR) proteins. We cloned and characterized twodmdR genes of Streptomyces coelicolor that were located in two different nonoverlapping cosmids. Functional ana-lysis of dmdR1anddmdR2was performed by deletion of each copy. Dele-tion of dmdR1resulted in the derepression of at least eight proteins and in the repression of three others, as shown by 2D proteome analysis.

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Thermodynamic parameters describing the conformational stability of the histidine-containing phosphocarrier protein fromStreptomyces coelicolor, scHPr, have been determined by steady-state fluorescence measurements of isothermal urea-denaturations, differential scanning calorimetry at different guanidinium hydrochloride concentrations and, independently, by far-UV circular dichroism measurements of isothermal urea-denaturations, and thermal denatura-tions at fixed urea concentrations.

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Streptomyces coelicolorandStreptomyces pilosusproduce desferrioxamine siderophores which are encoded by thedesABCDgene cluster.S. pilosusis used for the production of desferrioxamine B which is utilized in human medicine. We report the deletion of thedesA gene encoding a lysine decarboxylase in Streptomyces coelicolorA3(2).

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ThedmdR1gene ofStreptomyces coelicolorencodes an important regulator of iron metabolism. An antiparallel gene (adm) homologous to a develop-ment-regulated gene ofStreptomyces aureofacienshas been found to over-lap with dmdR1. Both proteins DmdR1 and Adm are formed in solid and liquid cultures of S. coelicolorA3(2).

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Volume et al. Bucca 2009 10, Issue 1, Article R5 Research Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Development and application of versatile high density microarrays for genome-wide analysis of Streptomyces coelicolor: characterization of the HspR regulon...

0p thulanh21 12-11-2011 57 2   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Analysis of gene expression in operons of Streptomyces coelicolor...

12p thulanh20 10-11-2011 56 4   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài:The global role of ppGpp synthesis in morphological differentiation and antibiotic production in Streptomyces coelicolor A3(2)...

18p thulanh19 05-11-2011 41 2   Download