Termination detection

Xem 1-12 trên 12 kết quả Termination detection
  • JR is a language for concurrent programming. It is an imperative language that provides explicit mechanisms for concurrency, communication, and synchronization. JR is an extension of the Java programming language with additional concurrency mechanisms based on those in the SR (Synchronizing Resources) programming language. It is suitable for writing programs for both shared- and distributed-memory applications and machines; it is, of course, also suitable for writing sequential programs.

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  • The CD53 antigen is a member of the tetraspanin membrane protein family that is expressed in the lymphoid-myeloid lineage. We have studied the implication of CD53 antigen in signal transduction by determining the effect of its ligation on the c-Jun N-terminal kinase (JNK) in different cell types. Ligation of the rat or human CD53 antigen induces a threeto fourfold transient activation of JNK activity that peaks at 3–5 min. The effect was detected by assaying the endogenous or exogenous (transfected) JNK activity....

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  • TheHfq (Host factor 1) polypeptide is anucleic acidbinding protein involved in the synthesis of many polypeptides. Hfq particularly affects the translation and the stability of several RNAs. In an earlier study, the use of fold recog-nition methods allowed us to detect a relationship between Escherichia coliHfq and the Sm topology. This topology was further validated by a series of biophysical studies and theHfqstructurewasmodelledonanSmprotein.Hfq forms ab-sheet ring-shaped hexamer.

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  • The N-terminal fusion peptide of Sendai virus F1envelope glycoprotein is a stretchof 14aminoacids,most ofwhichare hydrophobic. Following this region, we detected a segment of 11 residues that are strikingly similar to the N-terminal fusion peptide. We found that, when anchored to the mem-brane by palmitoylation of its N-terminus, this segment (WT-palm-19–33) induces membrane fusion of large unila-mellar liposomes toalmost the same extent as a segment that includes the N-terminal fusion peptide....

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  • A rootkit is a type of malicious software that gives the hacker "root" or administrator access to your network. They are activated before your system's operating system has completely booted up, making them extremely difficult to detect. Rootkits allow hackers to install hidden files, processes, and hidden user accounts. Hackers can use them to open back doors in order to intercept data from terminals, connections, and keyboards. A rootkit hacker can gain access to your systems and stay there for years, completely undetected.

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  • An exo-1,3-b-glucanase has been isolated from cultural filtrate of Trichoderma viride AZ36. The N-terminal sequence of the purified enzyme (m ¼ 61 ^ 1 kDa) showed no significant homology to other known glucanases. The 1,3-b-glucanase displayed high activity against laminarins, curdlan, and 1,3-b-oligoglucosides, but acted slowly on 1,3-1,4-b-oligoglucosides. No significant activity was detected against high molecular mass 1,3-1,4-bglucans. The enzyme carried out hydrolysis with inversion of the anomeric configuration.

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  • This method is real-time PCR, which can be a qualitative or quantitative assay since amplification and detection of amplified products occur simultaneously. Then, typing is performed. Typing is primarily used for epidemiologic investigations, for studies on pathogenesis such as multiple serotype infections, for unusual or especially severe infections, or for treatment approaches such as high titer γ-globulin. The nucleotide sequences from these fragments were determined by a DNA auto sequencer with fluorescent dideoxy chain terminators.

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  • The gene encoding an integrase of Mason–Pfizer monkey virus (M-PMV) is located at the 3¢-end of the pol open reading frame. The M-PMV integ-rase has not been previously isolated and characterized. We have now cloned, expressed, isolated, and characterized M-PMV integrase and com-pared its activities and primary structure with those of HIV-1 and other retroviral integrases. M-PMV integrase prefers untranslated 3¢-region-derived long-terminal repeat sequences in both the 3¢-processing and the strand transfer activity assays.

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  • We have developed a simple reporter assay useful for detection and analysis of mutations and agents influencing mRNA biogenesis in a gene length-dependent manner. We have shown that two transcription units sharing the same promoter, terminator and open reading frame, but differing in the length of their 3¢-untranslated regions, are differentially influenced by muta-

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  • The hypothesis thatL-glutamate (Glu) is an excitatory amino acid neurotransmitter in the mammalian central nervous system is now gaining more support after the suc-cessful cloning of a number of genes coding for the signaling machinery required for this neurocrine at synapses in the brain. These include Glu receptors (signal detection), Glu transporters (signal termination) and vesicular Glu trans-porters (signal output through exocytotic release).

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  • CASK-interactive protein1 is a newly recognized post-synaptic density protein in mammalian neurons. Although its N-terminal region contains several well-known functional domains, its entire C-terminal proline-rich region of 800 amino acids lacks detectable sequence homology to any previously characterized protein. We used multiple techniques for the struc-tural characterization of this region and its three fragments.

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  • The 2-hydroxycarboxylate transporter (2HCT) family of secondary trans-porters belongs to a much larger structural class of secondary transporters termed ST3 which contains about 2000 transporters in 32 families. The transporters of the 2HCT family are among the best studied in the class. Here we detect weak sequence similarity between the N- and C-terminal halves of the proteins using a sensitive method which uses a database con-taining the N- and C-terminal halves of all the sequences in ST3 and involves blastsearches of each sequence in the database against the whole database. ...

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