The dynamic lighting technique

The main aims of this research are: To determine the phase inversion temperatures for the formation of oil-in-brine nanoemulsions at various salt concentrations using conductivity measurements; To prepare the nano-emulsions at PIT points, and study their stability by measuring their droplet size, polydispersity and size distribution using dynamic light scattering technique; To confirm the DLS results using Cryo-SEM technique;...

324p runthenight07 01-03-2023 7 4   Download

Porcine heart cytoplasmic malate dehydrogenase (s-MDH) is a dimeric protein (2 · 35 kDa). We have studied equilibrium unfolding and refolding of s-MDH using activity assay, fluorescence, far-UV and near-UV circular dichroism (CD) spectroscopy, hydrophobic probe-1-anilino8-napthalene sulfonic acid binding, dynamic light scattering, and chromatographic (HPLC) techniques. The unfolding and refolding transitions are reversible and show the presence of two equilibrium intermediate states.

11p system191 01-06-2013 38 4   Download