The Fusarium wilt of banana
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The results showed that the designed primers were specific to only Foc isolates from race 1 and tropical race 4 (TR4). The detection efficiency of the designed primers was compared to other published primers through optimized SYBR green-based real-time PCR assay and nested PCR. The new primers could detect the Foc genomic DNA at a minimum of 5 pg and target pathogen in a symptomless banana sucker. The specificity and sensitivity of the new primers were comparable to the published real-time PCR primers and the nested PCR assay.
5p mudbound 10-12-2021 19 2 Download