The molecular level

In this thesis atomistic level modelling is conducted to investigate the design of an environmentally adaptable self-cleaning polymer paint coating that incorporates biomimetics to remain clean in various environmental setting. This work will be used to aid in the development of stay-clean polymer paint coating.

116p runthenight07 01-03-2023 7 3   Download

The objectives of the thesis: Identify the pathogenicity potential and classificate at species level of Colletotrichum spp. isolated from hot chilli in Vietnam; produce stable oligochitosan-silica nano, which potentially highly induced effectively the systemic resistance against anthranose disease on hot chilli, based on the combination of low molecular weight chitosan with nano silica.

28p capheviahe27 23-02-2021 29 4   Download

The quantum-chemical modeling mechanism of the [4+2]-cycloaddition reaction of 2,3 dimethylbuta-1,3-diene and methyl acrylate was conducted. Its qualitative aspects were analyzed at the molecular level by the program MOPAC2012 and semiempirical method RM1.

6p tocectocec 25-05-2020 7 1   Download

A molecular mechanism of the [3+2] cycloaddition has been explored using various DFT theoretical levels.

8p tocectocec 25-05-2020 6 1   Download

An immuno-homologue of maize Ca2+/calmodulin (CaM)dependent protein kinase with a molecular mass of 72 kDa was identified in pea. The pea kinase (PsCCaMK) was upregulated in roots in response to low temperature and increased salinity. Exogenous Ca2+ application increased the kinase level and the response was faster than that obtained following stress application. Low temperaturemediated, but not salinity-mediated stress kinase increase was inhibited by the application of EGTA and W7, a CaM inhibitor....

12p system191 01-06-2013 39 5   Download

Two genes (CM-AAT1 and CM-AAT2) with strong sequence homology (87% identity at the protein level) putatively involved in the formation of aroma volatile esters have been isolated from Charentais melon fruit. They belong to a large and highly divergent family of multifunctional plant acyl-transferases and show at most 21% identity to the only other fruit acyl-transferase characterized so far in strawberry. RT-PCR studies indicated that both genes were specifically expressed in fruit at increasing rates in the early and mid phases of ripening.

8p research12 01-06-2013 49 4   Download

Wilson disease (WD), an inherited disorder affecting copper metabolism, is characterized by hepatic cirrhosis and neuronal degeneration, which result from toxic levels of copper that accumulate in the liver and brain, respectively. We reported previously that the  1.3-kb promoter of the WD gene contains four metal response elements (MREs). Among the four MREs, MREa plays the most important role in the transcriptional activation of the WD promoter.

11p research12 01-06-2013 53 3   Download

ATP-regenerating enzymes may have an important role in maintaining ATP levels in mitochondria-like kinetoplast organelle and glycosomes in parasitic protozoa. Adenylate kinase (AK) (ATP:AMP phosphotransferase) catalyses the reversible transfer of thec-phosphate group from ATP to AMP, releasing twomolecules ofADP. This study describes cloningand functional characterizationof the gene encoding AK2fromagenomic libraryofLeishmaniadonovaniandalso its expression in leishmania promastigote cultures.

9p tumor12 20-04-2013 37 3   Download

The nature of cGMP transport in human erythrocytes, its relationship to glutathione conjugate transport, and pos-sible mediation by multidrug resistance-associated proteins (MRPs) have been investigated. MRP1, MRP4 and MRP5 are detected in immunoblotting studies with erythrocytes. MRP1and MRP5 are also detected in multidrug resistant COR-L23/R and MOR/R cells but at greatly reduced levels in the parent, drug sensitive COR-L23/P cells. MRP4 is detected in MOR/R but not COR-L23/R cells.

13p tumor12 20-04-2013 41 3   Download

The ethanol utilization pathway inAspergillus nidulansis a model system, which has been thoroughly elucidated at the biochemical, genetic and molecular levels. Three main ele-ments are involved: (a) high level expression of the positively autoregulated activator AlcR; (b) the strong promoters of the structural genes for alcohol dehydrogenase (alcA)and aldehyde dehydrogenase (aldA); and (c) powerful activa-tion of AlcRby the physiological inducer, acetaldehyde, produced from growth substrates such as ethanol and L-threonine. ...

10p tumor12 20-04-2013 21 2   Download

We purified pancreatic deoxyribonucleases I (DNases I) from three snakes, Elaphe quadrivirgata, Elaphe climaco-phoraandAgkistrodon blomhoffii, and cloned their cDNAs. Each mature snake DNase I protein comprised 262 amino acids. Wild-type snake DNases I with Leu130 were more thermally unstable than wild-type mammalian and avian DNases I with Ile130. After substitution of Leu130Ile, the thermal stabilities of the snake enzymes were higher than those of their wild-type counterparts and similar to mam-malian wild-type enzyme levels....

8p tumor12 20-04-2013 41 3   Download

Primary olfactory neuronal cultures exposed to odorant stimulation have previously exhibited concentration-related effects in terms of intracellular cAMP levels and adenylate cyclase activity [Ronnett, G.V., Parfitt, D.J., Hester, L.D. & Snyder, S.H. (1991)PNAS88, 2366–2369].Maximal sti-mulation occurred for intermediate concentrations, whereas AC activity declined for both low and high odorant con-centrations.We suspected that this behavior might be ascribed to the intrinsic response of the first molecular species concerned by odorant detection, i.e. the olfactory receptor itself....

8p fptmusic 16-04-2013 37 3   Download

Serine⁄threonine kinase 33 (STK33⁄Stk33) is a recently discovered gene whose inferred amino acid sequence translation displays characters typical for a calcium⁄calmodulin dependent kinase (CAMK). In this study we ana-lysed the STK33⁄Stk33RNA and protein distribution and the localization of the protein. TheSTK33⁄Stk33expression pattern resembles those of some related members of the CAMK group.STK33⁄Stk33displays a non-ubiquitous and, in most tissues, low level of expression.

15p fptmusic 12-04-2013 38 2   Download

By employing RT-PCR in conjunction with 3¢-RACE, a full-length cDNA encoding a novel zebrafish cytosolic sulfotransferase (SULT) was cloned and sequenced. Sequence analysis revealed that this zebrafish SULT (desig-nated SULT1 ST5) is, at the amino acid sequence level, close to 50% identical to human and dog SULT1B1 (thyroid hormone SULT). A recombinant form of zebrafish SULT1 ST5 was expressed using the pGEX-2TK bacterial expression system and purified from transformed BL21 (DE3) cells.

10p fptmusic 11-04-2013 42 3   Download

PKR is an interferon-induced serine-threonine protein kinase that plays an important role in the mediation of the antiviral and antiproliferative actions of interferons. PKR is present at low basal levels in cells and its expression is induced at the transcriptional level by interferons. PKR’s kin-ase activity stays latent until it binds to its activator. In the case of virally infected cells, double-stranded (ds) RNA serves as PKR’s activator.

15p awards 06-04-2013 31 2   Download

Lack of functional Fragile X mental retardation protein (FMRP) is the pri-mary cause of the Fragile-mental retardation syndrome in humans. In most cases, the disease results from transcriptional silencing of fragile mental retardation gene 1, fmr1, which encodes FMRP. However, a single mis-sense mutation (I304N) in the second KH domain of FMRP gives rise to a particularly severe case of Fragile X syndrome.

0p awards 05-04-2013 38 1   Download

Prion diseases are characterized by the accumulation of a pathogenic mis-folded form of a prion protein (PrP) encoded by the Prnpgene in humans. In the present study in zebrafish, two transcripts and the corresponding genes encoding prion proteins, PrP1 and PrP2, related to human PrP have been characterized with a relatively divergent deduced amino acid sequence, but a well preserved overall organization of structural prion pro-tein motifs.

14p awards 05-04-2013 39 2   Download

The extremely heat-stable 5¢-methylthioadenosine phos-phorylase from the hyperthermophilic archaeonPyrococcus furiosuswas cloned, expressed to high levels inEscherichia coli, and purified to homogeneity by heat precipitation and affinity chromatography. The recombinant enzyme was subjected to a kinetic analysis including initial velocity and product inhibition studies. The reaction follows an ordered Bi–Bi mechanism and phosphate binding precedes nucleo-side binding in the phosphorolytic direction....

11p awards 05-04-2013 39 2   Download

In our continued studies on corticotropin releasing factor receptor (CRFR1) signaling in the skin, we tested functional activity of CRFR1a, e, f, g and h isoforms after transfection to COS cells.Both membrane-bound and soluble variants are translatedin vivointofinal proteinproducts that undergo further post-translational modifications.

10p dell39 03-04-2013 40 3   Download

The physiological phenomenon that the antisweet taste effect of gymnemic acid (GA) is diminished by application of c-cyclodextrin (c-CD) to the mouth was evaluated at the molecular level using isothermal titration calorimetry, NMR and dynamic light scattering. These analyses showed that GA specifically binds to c-CD.

7p dell39 27-03-2013 48 4   Download