The ties that bind

The intrachloroplastic localization of post-transcriptional gene expression steps represents one key determinant for the regulation of chloroplast development. We have character-ized an RNA binding protein of 63kDa (RBP63) from Chlamydomonas reinhardtiichloroplasts, which cofraction-ates with stromal thylakoid membranes. Solubility proper-ties suggest that RBP63is a peripheral membrane protein.

8p research12 23-04-2013 34 3   Download

We report here on the formation of a complex between the two NrpR homologs present inMethanosarcina mazeiGo¨1 and their binding proper-ties to the nifHandglnK1 promoters. Reciprocal co-chromatography dem-onstrated that NrpRI forms stable complexes with NrpRII (at an NrpRI : NrpRII molar ratio of1 : 3), which are not affected by 2-oxo-glutarate.

14p viettel02 19-02-2013 27 2   Download