We indicate the stressed

Mitochondrial functional and structural impairment and generation of oxidative stress have been implicated in aging, various diseases and chemotherapies. This study analyzed azidothymidine (AZT)-caused failures in mitochondrial functions, in redox regulation and activation of the HIV-1 gene expression. We monitored intracellular concentrations of ATP and glutathione (GSH) as the indicators of energy production and redox conditions, respectively, during the time-course experiments with U937 and MOLT4 human lymphoid cells in the presence of AZT (0.05 mgÆmL)1) or H2O2 (0.01 mM) for 15–25 days.

7p system191 01-06-2013 42 3   Download

Metabolically unstable proteins are involved in a multitude of regulatory networks, including those that control cell signaling, the cell cycle and in many responses to physiological stress. In the present study, we have deter-mined the stability and characterized the degradation process of some members of the Gq class of heterotrimeric G proteins. Pulse-chase experi-ments in HEK293 cells indicated a rapid turnover of endogenously expressed Gaq and overexpressed Gaq and Ga16 subunits.

13p fptmusic 12-04-2013 37 2   Download

This paper reports the results obtained using the osmotic stress method applied to the purified cathodic and anodic hemoglobins (Hbs) from the catfishHoplosternum littorale, a species that displays facultative accessorial air oxygen-ation. We demonstrate that water potential affects the oxy-gen affinity ofH. littoraleHbs in the presence of an inert solute (sucrose). Oxygen affinity increases when water activity increases, indicating that water molecules stabilize thehigh-affinitystateof theHb.

0p awards 05-04-2013 41 3   Download