Báo cáo khoa học: Rational design of a conformation-switchable Ca2+- and Tb3+-binding protein without the use of multiple coupled metal-binding sites

Ca 2+ , as a messenger of signal transduction, regulates numerous target molecules via Ca 2+ -induced conformational changes. Investigation into the determinants for Ca 2+ -induced conformational change is often impeded by cooperativity between multiple metal-binding sites or protein oligomeriza-tion in naturally occurring proteins.