Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli

The adenosine A2a receptor belongs to the seven trans-membrane helix G-protein-coupled receptor family, is abundant in striatum, vasculature and platelets and is involved in several physiological processes such as blood pressure regulation and protection of cells during anoxia. For structural and biophysical studies we have expressed the human adenosine A2areceptor (hA2aR) at high levels inserted into the Escherichia coliinner membrane, and established a puri®cation scheme.