Common G102S polymorphism in chitotriosidase differentially affects activity towards 4-methylumbelliferyl substrates Anton P. Bussink1, Marri Verhoek1, Jocelyne Vreede2, Karen Ghauharali-van der Vlugt1, Wilma E. Donker-Koopman1, Richard R. Sprenger1, Carla E. Hollak3, Johannes M. F. G. Aerts1 and Rolf G. Boot1
1 Department of Medical Biochemistry, Academic Medical Center, University of Amsterdam, The Netherlands 2 Van’t Hoff Institute for Molecular Sciences, University of Amsterdam, The Netherlands 3 Department of Internal Medicine, Academic Medical Center, University of Amsterdam, The Netherlands
Keywords chitinase; chitotriosidase; Gaucher disease; molecular dynamics simulation; single nucleotide polymorphism
Correspondence R. G. Boot, Department of Medical Biochemistry, Academic Medical Center, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands Fax: +31 2069 15519 Tel: +31 2056 65157 E-mail: r.g.boot@amc.uva.nl
(Received 20 May 2009, revised 10 July 2009, accepted 5 August 2009)
doi:10.1111/j.1742-4658.2009.07259.x
Chitotriosidase (CHIT1) is a chitinase that is secreted by activated macro- phages. Plasma chitotriosidase activity reflects the presence of lipid-laden macrophages in patients with Gaucher disease. CHIT1 activity can be con- veniently measured using fluorogenic 4-methylumbelliferyl (4MU)–chitotri- the substrate, however, nonsaturating oside or 4MU–chitobioside as concentrations have to be used because of apparent substrate inhibition. Saturating substrate concentrations can, however, be used with the newly designed substrate 4MU–deoxychitobioside. We studied the impact of a known polymorphism, G102S, on the catalytic properties of CHIT1. The G102S allele was found to be common in type I Gaucher disease patients in the Netherlands ((cid:2) 24% of alleles). The catalytic efficiency of recombi- nant Ser102 CHIT1 was (cid:2) 70% that of wild-type Gly102 CHIT1 when measured with 4MU–chitotrioside at a nonsaturating concentration. How- ever, the activity was normal with 4MU–deoxychitobioside as the substrate at saturating concentrations, consistent with predictions from molecular dynamics simulations. In conclusion, interpretation of CHIT1 activity mea- surements with 4MU–chitotrioside with respect to CHIT1 protein concen- trations depends on the presence of Ser102 CHIT1 in an individual, complicating estimation of the body burden of storage macrophages. Use of the superior 4MU–deoxychitobioside substrate avoids such complica- tions because activity towards this substrate under saturating conditions is not affected by the G102S substitution.
Introduction
Abbreviations 4MU, 4-methylumbelliferyl; CHIT1, chitotriosidase; GD, Gaucher disease; MD, molecular dynamics; r.m.s.f., root mean square fluctuations.
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Gaucher disease (GD; MIM 230800) is a recessively inherited disease that is caused by deficient activity of the lysosomal glucocerebrosidase (MIM 606463, EC 3.2.1.45) [1]. Although glucocerebrosidase is present in lysosomes of all cell types, type I GD patients develop exclusive glucosylceramide storage in macrophages. It is believed that the storage material in macrophages stems from the breakdown of exogenous lipids derived from the turnover of blood cells. Characteristic lipid- laden macrophages, Gaucher cells, accumulate in the liver, spleen and bone marrow. GD is characterized by hepatosplenomegaly, haematological abnormalities and
A. P. Bussink et al.
Substrate specificity of G102S chitotriosidase
and coworkers, and Beutler
the artificial
factor, angiotensin
Gaucher patients is therefore recommended. A further polymorphism resulting in a G102S substitution exists in the CHIT1 gene (MIM 600031). This was first reported by Gray and collaborators [30a]. Desnick collaborators and reported the common occurrence of the G102S allele among GD patients and normal subjects [31,32]. The Ser102 CHIT1 enzyme was found to show reduced substrate catalytic efficiency towards 4MU–chitotrioside compared with wild-type enzyme [31]. We have investigated in detail the frequency of the G102S CHIT1 allele and the impact of amino acid substitution on the catalytic efficiency towards various substrates. The interpretation of plasma chitot- riosidase activities when measured with various substrates with respect to estimating disease severity is discussed.
Results
Frequency of CHIT1dup24 and CHIT1 G102S lysosomal
for respectively, replacement [21–25]
The CHIT1 genotype was determined in a large number of Gaucher patients of European ancestry (n = 86). Among the Gaucher patients, 3.5 and 41.7% were the homozygous or heterozygous, G102S mutation, with an allele frequency of 0.24 (41 ⁄ 172). Among the same patients, 6 and 27% were homozygous or heterozygous for the dup24 allele respectively, with an allele frequency of 0.20 (35 ⁄ 172). The numbers of detected homozygotes for the G102 allele and the dup24 allele were consistent with the Hardy Weinberg equilibrium. Sequencing the CHIT1 gene of selected cases revealed that, in the GD patient cohort, all four conceivable CHIT1 alleles occurred (allele containing duplication without G102S mutation, allele containing duplication with G102S mutation, allele without duplication and without G102S mutation, allele without duplication and with G102S mutation).
Enzymatic activity of chitotriosidase towards various artificial substrates
results
solely express Individuals that
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skeletal involvement [1,2]. There is a remarkable spec- trum of clinical severity among type I GD patients. The limited correlation of genotype with phenotype has stimulated a search for secondary biochemical markers that might indicate disease severity [3]. The importance of markers reflecting disease progression and correction increased further with the introduction of enzyme replacement therapy [4] and substrate reduc- tion therapy [5,6]. Several serum abnormalities have been documented in GD patients (i.e. macrophage colony-stimulating converting enzyme, tartrate-resistant acid phosphatase, CD163 and CCL18) [7–9]. The most striking abnormality is elevated plasma chitotriosidase (CHIT1) activity [10]. CHIT1 is a chitinase (EC 3.2.1.14) secreted by alterna- tively activated human macrophages [11,12]. CHIT1 is produced as a 50 kDa protein, consisting of a chitin- binding domain, a hinge region and a 39 kDa catalytic domain in which enzymatic activity resides [13]. The enzyme is secreted into the circulation as 50 kDa pro- tein [14]. Plasma chitotriosidase activity is increased in [15–19] and nonlysosomal diseases several [20]. In untreated GD patients, the median activity is (cid:2) 600-fold that in normal controls [10]. Plasma CHIT1 activity has proven useful for monitoring disease sever- ity and the effectiveness of therapy in GD, including enzyme and, more therapy recently, substrate reduction therapy [26,27]. In 2004, the International Collaborative Gaucher Group for- mally recommended plasma chitotriosidase activity as the biomarker of choice for evaluating GD patients and monitoring the effectiveness of enzyme replace- ment therapy. Monitoring therapeutic response by measuring plasma chitotriosidase activity has two limi- tations. Assaying CHIT1 activity using commercially available substrates is complicated by the existence of apparent substrate inhibition caused by transglycosi- dase activity [28], because of this, activity cannot be measured at saturating substrate concentrations and does not accurately reflect chitotriosidase protein levels. A novel substrate, 4-methylumbelliferyl (4MU)– deoxychitobiose, has been developed that allows more accurate and sensitive measurement of chitotriosidase [28,29]. Another pitfall from the complete absence of enzymatic activity in (cid:2) 6% of individuals with European ancestry and even higher percentages in is individuals of Asian ancestry [30–32]. This trait caused by homozygosity for a 24 bp duplication in exon 10, designated dup24, in the CHIT1 gene, pre- venting formation of active enzyme [30]. Plasma CHIT1 levels in heterozygotes for this null allele underestimate the actual presence of Gaucher cells in the CHIT1 genotype in patients. Determination of Chitotriosidase activity in plasma samples of 47 type I GD patients with an established CHIT1 genotype was measured using 4MU–chitotrioside and 4MU– deoxychitobiose as substrates. A significant correlation between the G102S genotype and activity towards the two artificial substrates became apparent when analysing the results for individuals lacking the dup24 the allele (Fig. 1). wild-type Gly102 enzyme (genotype G ⁄ G) display the highest 4MU–chitotrioside ⁄ 4MU–deoxychitobioside
A. P. Bussink et al.
Substrate specificity of G102S chitotriosidase
P = 0.0078
P = 0.02
P = 0.0002
0.8
less
i
0.6
/
0.4
0.2
) o b y x o e d o i r t ( o i t a R
0.0
A/A
G/A
G/G
4MU–deoxychitobiose substrate
Fig. 1. Activity of plasma CHIT1 towards artificial substrates according to CHIT1 genotype. Ratios of activities towards sub- strates 4MU–chitotrioside and 4MU–deoxychitobioside as mea- sured for plasma samples according to patient genotype. Horizontal bars represent median values.
chitotriosidase. To test the possibility that the mutant enzyme is indeed glycosylated, we compared recombi- nant-produced 39 kDa wild-type and Ser102 CHIT1 using western blot analysis. As shown in Fig. 2A, the intense, mutant enzyme shows an additional, cross-reactive protein with a molecular mass slightly higher than 39 kDa. To assess the nature of this addi- tional isoform, we subjected the recombinant proteins to endoglycosidase F digestion. Figure 2B shows that the additional isoform of the mutant enzyme is sensi- tive to endoglycosidase F digestion, suggesting that it is glycosylated. Following electrophoretic protein sepa- ration in a SDS-acrylamide gel, two isoforms could also be visualized by detecting hydrolysis of the fluo- rogenic (Fig. 2C, upper). Apparently, both isoforms are enzymatically active.
Next, plasma samples of Gaucher patients with dif- ferent genotypes (G ⁄ G, G ⁄ A and A ⁄ A), and lacking the dup24 allele, were subjected to western blot analy- sis. Figure 2D shows that in the case of plasma from patients that strictly express the wild-type enzyme of 50 kDa, only a single cross-reactive band is detected. However, samples from patients that carry the mutant allele display an additional cross-reactive band above the 50 kDa protein, which was found to be sensitive to endoglycosidase F digestion (not shown). The addi- tional band is more intense in homozygotes for Ser102 CHIT1 (A ⁄ A) than in heterozygotes (G ⁄ A) (Fig. 2D).
Specific activity of wild-type and Ser102 CHIT1
individuals (trio ⁄ deoxybio) activity ratios, whereas that express solely the Ser102 enzyme (genotype A ⁄ A) have substantially lower trio ⁄ deoxybio activity ratios. Heterozygotes (genotype G ⁄ A) show intermediate values. In the case of carriers of the dup24 allele, a broad range of reduced trio ⁄ deoxybio activity ratios was observed (not shown). As established by sequencing of large segments of CHIT1 genes, this is explained by the fact that in some individuals the G102S mutation is on the same allele as the duplication and only wild- type protein is produced, whereas in others the G102S mutation is on the wild-type allele and G102S substi- tuted enzyme is solely present.
and 4MU–deoxychitobiose was
Next, the activity of recombinant produced 39 kDa wild-type and Ser102 CHIT1 towards 4MU–chitotrio- side determined. Recombinant Ser102 CHIT1 showed a clearly reduced (75% of wild-type enzyme) trio ⁄ deoxybio activity ratio, mimicking findings made with plasma enzymes. This suggests that the catalytic efficiency of Ser102 CHIT1 towards 4MU–deoxychitobioside is normal, but is slightly impaired towards 4MU–chitotrioside. Of note, enzyme activity measured with the substrate 4MU–chitobioside revealed that the G102S substitu- tion, either in plasma enzyme or recombinant chitotri- osidase, did not affect markedly the bio ⁄ deoxybio activity ratio (not shown).
Glycosylation of G102S chitotriosidase
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The G102S mutation creates a potential glycosylation site at Asn100 within the 39 kDa catalytic domain of To determine whether the G102S substitution in CHIT1 affects catalytic efficiency towards 4MU–chito- trioside and 4MU–deoxychitobioside, the specific activ- ity of COS-produced recombinant wild-type and Ser102 CHIT1 was studied. Unfortunately, accurate direct measurement of protein concentration was not feasible given the low quantities of recombinant enzymes available. Using SDS ⁄ PAGE and western blotting, the catalytic efficiency of 39 kDa wild-type and Ser102 CHIT1 was compared (Fig. 3). Applying an equal amount of activity towards 4MU–deoxychito- bioside for both enzymes resulted in equally intense amounts of cross-reactive material. However, applying an equal amount of activity towards 4MU–chitotrio- side for both enzymes resulted in less cross-reactive in the case of wild-type enzyme (60–80% material compared with mutant enzyme). Thus, the specific activity of Ser102 CHIT1 towards 4MU–chitotrioside appears to be reduced. Recently, a label-free LC-MS method was developed that allows absolute quantifica- tion of CHIT1 protein in plasma specimens [33].
A. P. Bussink et al.
Substrate specificity of G102S chitotriosidase
the
A
B
substrate 4MU–deoxychitobioside Fortunately, cannot be transglycosylated and shows Michaelis– Menten kinetics allowing determination of Km. The Km of Ser102 CHIT1 for the 4MU–deoxychitobioside (determined by means of Eadie–Hofstee plotting and linear regression) is 102 ± 6 lm, substantially higher than that of wild-type enzyme (43 ± 1 lm). Both recombinant proteins were found to be active towards the natural chito-oligomer chitohexaose releasing both chitobiose and chitotriose moieties from the chitohexa- ose (Table 1). The ability of G102S chitotriosidase to hydrolyse this natural chitin oligomer appeared only marginally reduced compared with wild-type enzyme.
C
Modelling of the G102S substitution
D
Fig. 2. Analysis of glycosylation of Ser102 CHIT1. Western blot and in-gel activity analyses of the glycosylation pattern of both recombinant and plasma proteins. (A) Western blot of recombinant 39 kDa CHIT1 proteins. (B) Effect of digestion with endoglycosi- dase F. (C) In-gel activities of both proteins at increasing concentra- tions (upper) with parallel western blot signal (lower). (D) Western blot of plasma CHIT1 isoforms in relation to Gaucher patients’ genotypes.
The 3D structure of CHIT1 has been extensively stud- ied using crystallography [34,35] and a reliable predic- tion can therefore be made for the enzyme structure containing a serine instead of glycine at amino acid 102. The protein was shown to adopt a highly stabi- lized (b ⁄ a)8-fold, also known as a triosephosphate isomerase barrel. Mutation of the glycine into serine did not alter the overall structure, as concluded from the near superimposability of the energy-minimalized structures of the 102G and 102S proteins (r.m.s.d. = 0.02 A˚ ). However, because Ser102 is located close to the binding cleft, we investigated whether possible hydrogen-bonding interactions of the serine hydroxyl in altered substrate binding. Because might result the G102S mutation was shown to affect hydrolysis of the chitotrioside substrate and to a lesser extent the chitobioside substrate, it was hypothesized that differ- ences in binding of the third sugar are responsible for the observed differences in activity.
plasma containing sample both a hydrogen-bonding reveals
Therefore, the published crystal structure of the chi- totriosidase–allosamidin complex was carefully exam- ined [35]. Allosamidin is a potent chitinase inhibitor consisting of two N-acetylglucosamine residues and a group that mimics the transition-state analogue and can therefore be used to assess positioning of the sec- ond and third sugar residues in the binding cleft. The interaction structure between the N-acetyl moiety of the third sugar and Asn100.
CHIT1 protein concentrations in plasma samples were measured in both a heterozygous individual and a homozygous wild-type individual. The specific activity towards 4MU–chitotrioside was lowest in the case of enzymes the (3.25 mmolÆmg–1Æh–1), and highest in plasma containing only wild-type CHIT1 (4.09 mmolÆmg–1Æh–1). This con- firms the observation (Fig. 3) that Ser102 CHIT1 is only slightly impaired in activity towards 4MU–chito- trioside.
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the Other enzymatic features of wild-type and Ser102 CHIT1 were comparatively investigated. Both enzymes showed apparent substrate inhibition with 4MU–chito- trioside as substrate, a phenomenon caused by transglycosylation of this substrate (not shown). In order to evaluate differences between both pro- teins we performed molecular dynamics (MD) simula- tions in which atoms are allowed to interact for a time under known laws of physics, providing insight into the motion of atoms. Simulations of both native wild-type and mutant unglycosylated structures were performed. The 10 ns MD runs show a considerable overall rigidity of secondary structures, as shown by
A. P. Bussink et al.
Substrate specificity of G102S chitotriosidase
Equal input based on activity towards 4MU-chitotrioside
Equal input based on activity towards 4MU-deoxychitobioside
Gly102
Ser102
Gly102
Ser102
*
** *
*
** *
200
)
%
150
**
100
**
50
*
*
*
*
( l a n g i s e v i t a l e R
0
Gly102
Ser102
Gly102
Ser102
Fig. 3. Apparent specific activity of recombi- nant wild-type and Ser102 CHIT1. (Upper) Equal amounts of activity of recombinant wild-type and Ser102 CHIT1, either with 4MU–chitrioside (left) or 4MU–deoxychito- bioside (right) were subjected to western blot analysis. (Lower) Relative signal inten- sity, quantified as described in Materials and Methods (*relative signal lower band, **rel- ative signal upper glycosylated band). The intensity of the Gly102 enzyme is set at 100% with each substrate.
Correlation of wild-type and Ser102 CHIT1 with severity of GD manifestation
activity measurements, statistical root mean square fluctuations (r.m.s.f., a measure of flexibility) of 0.05–0.15 nm, consistent with the com- pact, highly stabilized structure of the (b ⁄ a)8 barrel. Furthermore, the catalytic glutamic acid is accessible to solvent, compatible with hydrolase activity. Com- parison of the residue-specific r.m.s.f. between wild- type and G102S chitotriosidase shows a markedly decreased mobility for residues 96–104 in the G102S protein, corresponding to the loop separating b3 and a3 containing Asn100 (Fig. 4A). Visual inspection of the MD trajectories shows that the hydroxyl oxygen of Ser102 is able to form additional hydrogen bonds with the peptide backbone at Phe101 and Lys105 and the side chain of both Gln104 and Lys105, resulting in demobilization of the loop (Fig. 4B). Because the G102S substitution results in a marked decrease in flexibility it is conceivable that the sugar at the -3 position may no longer be stabilized by Asn100, which is likely to result in a lower activity of the enzyme towards the 4MU–chitotrioside substrate. It remains unclear, however, how the mutation affects the binding constant of the enzyme for 4MU–deoxy- chitobioside.
Table 1. Formation of fragments from chitohexaose (expressed in lM) by wild-type and Ser102 CHIT1.
CHIT1 is a useful biomarker with which estimate dis- ease severity and monitor the effectiveness of enzyme replacement therapy. Because CHIT1 is secreted from pathological lipid-laden Gaucher cells that accumulate predominantly in the liver, spleen and bone marrow, a correlation between enzyme activity and both excess liver and spleen volume has been proposed and dem- onstrated [25]. The findings presented above show that the G102S substitution results in an underestimation of the amount of CHIT1 protein when measured enzy- matically with 4MU–chitotrioside. In light of this, we looked for a cohort of type I GD patients lacking the dup24allele and with an intact spleen, the correlation of excess liver volume and plasma CHIT1 employing both 4MU–chitotrioside and 4MU–deoxychitobiose as substrates. We observed a q of 0.58 (P = 0.0004) when plasma chitotriosidase activities were measured with 4MU–chitotrioside. Using 4MU–deoxychitobiose for significance increased to 0.66 (P < 0.0001). Thus, the correlation between excess liver volume and CHIT1 activity indeed improves when using 4MU–deoxychitobiose as sub- strate for enzyme measurements.
Discussion
Substrate (GlcNAc)6 60 lM
Substrate (GlcNAc)6 120 lM
Wild-type
Ser102
Wild-type
Ser102
23.6 27.7 13.3 33.0
28.0 40.2 15.9 21.0
19.7 20.0 13.5 90.0
28.1 36.1 19.2 88.0
(GlcNAc)2 (GlcNAc)3 (GlcNAc)4 (GlcNAc)6
in various groups of
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Coinciding with our investigation, the research groups of Desnick and Beutler independently characterized CHIT1genotypes individuals [31,32]. Like us, Desnick and coworkers noted the common occurrence among GD patients and normal subjects of the dup24 and G102S alleles [31]. Interest- ingly, the observed frequency of the G102S allele was
A. P. Bussink et al.
Substrate specificity of G102S chitotriosidase
0.3
A
0.2
)
m n ( F S M R
0.1
0.0
70
80
90
100
110
120
Residue number
(n = 180), African (n = 150) and Asian (n = 904) subjects, respectively. The results of our study with Gaucher patients of European ancestry are remarkably consistent with the reports by the groups of Desnick and Beutler. The frequency of the G102S allele in the patient population studied by us was 0.24 and that of the dup24 allele was 0.20. Of note, we observed that the 24 bp duplication and G102S mutation are not strictly linked and that all possible combinations of the CHIT1 alleles occur. It thus seems most likely that the two mutations in CHIT1 are ancient and that already among the founders of non-African ethnic groups carriers of all four different CHIT1 alleles must have existed.
B
The consequences of
is shown in nm on the y-axis)
Fig. 4. Structural implications of the G102S mutation. (A) r.m.s.f. in the affected domain (residue numbers are shown on the x-axis, r.m.s.f. in wild-type (grey) and Ser102 CHIT1 (black). Values represent averages obtained from three independent runs. (B) Superposition of wild-type and mutant structures (grey is wild-type). Amino acids 70-95 of both enzymes are coloured according to r.m.s.f. on a scale from blue (r.m.s.f. = 0 nm) to red (r.m.s.f. = 0.25 nm). The location of the mutation is highlighted (green in wild-type protein, yellow in Ser102 CHIT1). Side chains of Ser102 and Lys105 with hydrogen-bonded interactions are shown.
the artificial
structural enzyme. Indeed, the
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(cid:2) 0.3 in subjects of various ancestries, including African. This is in sharp contrast to the situation for the dup24 allele which is far less frequent among indi- viduals of African extraction compared with subjects of European ancestry [32,36,37]. Concomitantly, Beu- tler and coworkers determined, in an impressive series of individuals, the frequency of the dup24 allele, being 0.56 (n = 2054) in subjects of Asian ancestry, 0.17 (n = 984) in subjects of European ancestry and 0.07 (n = 536) in subjects of African ancestry [32]. They also reported high G102S allele frequencies for various ethnic groups, being 0.27, 0.26 and 0.24 for European the G102S substitution in CHIT1 for its enzymatic efficiency are of interest. Desnick and coworkers reported a markedly (about fourfold) reduced catalytic activity of Ser102 CHIT1 towards substrates 4MU–chitotrioside [31]. Although, Beutler and collaborators did not find significantly reduced chitotriosidase activity in plasma of carriers for the G102S substitution, it appears that in individuals homozygous for the G102S allele the plasma chitotriosidase activity is clearly reduced com- pared with individuals lacking this allele [32]. In our the specific activity of recombinant Ser102 hands, CHIT1 towards 4MU–chitotrioside is (cid:2) 70% of nor- mal. A similar extent of reduction in specific activity was noted for plasma-derived Ser102 CHIT1. Desnick and coworkers compared the specific activity of wild- type and Ser102 CHIT1 in media of COS-transfected cells using silver-staining after gel electrophoresis. In their case, the protein signal staining intensities of aliquots containing almost equal 4MU–chitotrioside hydrolysing activity were much higher in the case of Ser102 CHIT1 than wild-type enzyme. It was con- cluded that Ser102 CHIT1 showed only 23% of wild- type catalytic activity. In our hands, the differences between wild-type and Ser102 CHIT1 in activity towards 4MU–chitotrioside are much smaller. A possi- ble, quite trivial, explanation for the apparent differ- ences in findings among various research groups may be that very low substrate 4MU–chitotrioside concen- trations have to be used in assays of CHIT1 activity. The binding constant of Ser102 CHIT1 for this sub- strate may very well differ from that of wild-type analyses Gly102 employed show an increase in rigidity in the Ser102 protein in a region associated with binding of the third sugar of 4MU–chitotrioside, whereas the rest of the protein appears relatively unaffected. Unfortunately, this constant cannot be experimentally determined the because of the ongoing transglycosylation of
A. P. Bussink et al.
Substrate specificity of G102S chitotriosidase
substrate patients: Gaucher uncorrected
the binding constant
chitohexaose indicates
[28]. However, using 4MU–chitotrioside 4MU–deoxychitobioside as the substrate, which cannot undergo transglycosylation, a substantially higher Km in the case of Ser102 CHIT1 was observed by us. It is for therefore conceivable that 4MU–chitotrioside is indeed affected by the G102S substitution in CHIT1 and that, in combination to this, slight differences in assay concentration of 4MU– chitotrioside among research groups might generate different results for relative specific activity of Ser102 CHIT1. Our finding that the G102S substitution has only a very small effect on hydrolysis of the natural that chito-oligosaccharide Ser102 CHIT1 is not intrinsically impaired in hydro- lytic activity. The same is suggested by the normal activity of the enzyme towards 4MU–deoxychito- bioside. The consequence of still useful. Awareness of substrate is
q = 0.58 in (P = 0.0004), corrected q = 0.65 (P = 0.0001), the latter being almost identical to q = 0.66 (P < 0.0001) as observed for chitotriosidase data obtained with 4MU–deoxychitobioside as the substrate. Obviously, it should be realized that the correction factor may differ between research groups, being highly dependent on the precise assay conditions, in particular 4MU–chito- trioside concentration. Moreover, it should be kept in mind that, although appealing, such correction is not feasible in carriers of both the G102S allele and dup24 allele. In such cases it is not known a priori whether the two mutations are at the same or distinct CHIT1 alleles. In the former situation no correction should be made and in the latter the correction should be by a factor of 1.6 when using our assay conditions. It should be emphasized that in the longitudinal manage- ment of individual Gaucher patients the 4MU–chitotri- oside the limitations of the 4MU–chitotrioside substrate is of importance to facilitate consistency in the assay in order to correctly assess visit-to-visit variations in plasma chitotriosidase activity in individual patients with or without this polymorphism. the partial glycosylation of Ser102 CHIT1 is still unclear. Our investigation did not point to a major difference in enzymatic activity between glycosylated and unglycosylated enzyme when measured with 4MU–deoxychitobioside as substrate. Obviously, it cannot be excluded that the glycosylated isoform is more rapidly (lectin-mediated) cleared from the circulation. In conclusion,
in an underestimation of disease
use the of
the G102S substitution in CHIT1 occurs commonly among individuals of European ancestry, including Gaucher patients. Because this sub- stitution negatively affects the activity of CHIT1 towards 4MU–chitotrioside, plasma enzyme activities measured with this substrate may, in some individuals, insufficiently reflect chitotriosidase protein, the latter being related to the presence of storage cells. This may result severity. Because of its unambiguity towards the G102S substi- tution, 4MU–deoxychitobioside the substrate has to be recommended for an optimal inter- pretation of plasma chitotriosidase activities in relation to monitoring disease severity. serve as acceptor
Materials and methods
Peripheral blood was collected from type I GD patients and normal subjects evaluated at the Academic Medical Center, University of Amsterdam. All patients gave consent for the use of samples for the purpose of the study. Base- line data on sex, age, splenectomy, severity score index and genotype were recorded. Liver volumes were derived from computerized tomography images as described earlier [24]. Excess liver volume was derived by subtracting a notional ‘expected’ liver volume (2.5% of body weight) from the observed liver volume.
Given the current application of plasma CHIT1 as measure for the body burden of Gaucher cells in GD patients and its use to assess disease severity and effi- cacy of therapeutic intervention, the genetic heterogene- ity in the CHIT1 gene is of importance. This has been elegantly pointed out by Desnick and coworkers [31]. Interpretation of plasma CHIT1 activities, especially when determined with 4MU–chitotrioside as substrate, should take into account the CHIT1 genotype of an individual. Importantly, the newly developed substrate 4MU–deoxychitobiose offers a convenient solution. The catalytic efficacy towards this substrate seems not to be affected by the G102S substitution. The fact that 4MU–deoxychitobiose cannot in transglycosylation offers further advantages such as the use of saturating substrate concentration. When chitotriosidase Patient specimens
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is used as a comparator correction of measured plasma between patients, CHIT1 for patients carrying the G102S allele may be considered. According to the observed reduction in specific activity of Ser102 CHIT1, correction would imply multiplying levels of plasma chitotriosidase activity measured with 4MU–chitotrioside under our assay conditions by a factor of 1.3 in the case of carri- ers for the G102S allele and by a factor of 1.6 in the case of homozygotes for G102S allele. Applying such a correction to our dataset improved the correlation between excess liver volume and plasma chitotriosidase
A. P. Bussink et al.
Substrate specificity of G102S chitotriosidase
CHIT1 cDNA was cloned as described previously [13]. A fragment of the cDNA encoding the 39 kDa catalytic domain was used for recombinant protein production. The G102S point mutation was introduced directly into the wild- type CHIT1 cDNA in the expression plasmid, pcDNA3.1, using a fragment containing the G102S amplified from an individual that contained this polymorphism. Large-scale production and purification of the wild-type and mutant cDNA expression plasmids were performed using Qiagen Plasmid Midi Kits (Qiagen, Venlo, The Netherlands).
transfection,
Chitotriosidase activity in plasma samples, stored at )80 (cid:2)C, was measured with the natural chitin fragment chitohexaose or the fluorogenic substrates 4MU–chitotrioside, 4MU–chi- tobioside and 4MU–deoxychitobioside. Chitohexaose was obtained from Seikagaku Corp. (Tokyo, Japan), 4MU– chitotrioside and 4MU–chitobioside were from Sigma (St Louis, MO, USA). 4MU–deoxychitobiose was synthesized as described previously [28], (contact j.m.aerts@amc.uva.nl for availability). Briefly, for the enzyme activity assay with 4-MU–substrates, 25 lL serum, diluted with BSA ⁄ NaCl ⁄ Pi (1 mgÆmL–1) and 100 lL substrate mixtures were incubated for 20 min at 37 (cid:2)C. To determine activity ratios, the sub- contained 0.0113 mm 4MU–chitotriose, strate mixtures 0.027 mm 4MU–chitobiose or 0.250 mm 4MU–deoxychito- biose and 1 mgÆmL–1 BSA in McIlvain buffer (pH 5.2). Reactions were stopped with 2.0 mL of 0.3 m glycine NaOH buffer (pH 10.6) and the formed 4MU was detected fluoro- metrically (excitation at 366 nm; emission at 445 nm). Only < 10% difference in the duplicates was allowed. One unit (U) of activity is defined as 1 nmol of substrate hydrolysed per hour. Activity towards the natural oligo-saccharide chitohexaose was measured using a HPLC method as described previously [38].
COS-7 cells were plated in complete media in six-well plates at a cell density of 1–3 · 105 cells per well and left overnight to achieve the desired cell concentration of 50% to 80% confluency. On the day of the complete media in each well was replaced with 1 mL of serum-free media. Transient transfection with the expres- sion plasmid pcDNA3.1 containing the wild-type or mutant CHIT1 cDNA was achieved using FuGene 6 trans- fection reagent according to the manufacturer’s protocol (Roche Applied Science, Indianapolis, IN, USA). After 72 h, the media was collected and subjected to chitotriosi- dase assays.
Plasma chitotriosidase enzyme assays Isolation and expression of normal and mutant CHIT-1 DNA
An antiserum raised against recombinant produced chitotri- osidase [11] was used to visualize chitotriosidase protein on western blots. The presence of N-linked glycans was deter- mined by monitoring the shift in molecular mass of chitot- riosidase upon digestion with endoglycosidase F (New England Biolabs, Frankfurt, Germany).
In-gel enzymatic assay Western blot analysis
In-gel chitinase activity was determined in a 12% polyacryl- amide gel containing SDS, run in the absence of b-mer- captoethanol. Renaturing of separated proteins was accomplished by incubating the gel for 16 h at room tem- perature in a casein-containing suspension (2.5 gÆL–1 casein, 20 mm Tris, 2 mm EDTA, pH 8.5). Prior to exposure to artificial substrate the gel was washed three times in 30 mm NaAc ⁄ HAc (pH 5.2). The gel was soaked in 250 lm 4MU–deoxychitobiose for 1 min, after which the fluores- cent signal was determined at various exposure times in a Roche Lumi-Imager with settings optimized for 4MU fluorescence.
Determination of specific activity of normal and Ser102 CHIT1
DNA was isolated from peripheral blood using the Gentra PureGene kit (Minneapolis, MN, USA). Detection of the common dup24 insertion in exon 10 of the CHIT1 gene (NM_003465.1) was performed as described previously [30]. The G102S mutation was detected by polymerase chain reaction amplification of the appropriate fragment (primers: RB203 5¢-GGCAGCTGGCAGAGTAAATCC-3¢ and RB204 5¢-CCCAGAAGGAAATTCAGCCC-3¢) and sequencing (Big Dye Terminator sequencing kit, Applied Biosystems, Foster City, CA, USA, according to manufac- turers protocol on an Applied Biosystems 377A automated DNA sequencer).
The specific activity of recombinantly produced wild-type and Ser102 CHIT1 was assessed by comparing the inten- sity of cross-reactive material with western blot analysis using a similar input of enzymatic activity of both enzymes. Autoradiographs were quantified by imaging densitometry and analysed using imagequant-tl software (ImageQuant; Molecular Dynamics, Sunnyvale, CA, USA) or quantity one analysis software (Bio-Rad Labo- ratories, Hercules, CA, USA). For comparison, we used a pure standard of recombinant chitotriosidase, produced previously on a large scale and for which specific activity had been determined by protein measurement [39]. The specific activity of plasma wild-type and Ser102 CHIT1 was also determined using label-free LC-MS, as described previously [33]. Plasma was analysed from an individual expressing both G102S and wild-type chitotriosidase and an individual expressing only wild-type enzyme.
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CHIT1 genotyping
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Substrate specificity of G102S chitotriosidase
Modelling of the G102 substitution and MD simulation research was funded by the Academic Medical Center, University of Amsterdam.
References
1 Beutler E & Grabowski G (2001) Gaucher disease. In The Metabolic and Molecular Bases of Inherited Disease (Scriver CR, Beaudet AL, Sly WS & Valle D eds), pp. 3635–3668. McGraw-Hill, New York, NY.
2 Cox TM & Schofield JP (1997) Gaucher’s disease: clinical features and natural history. Baillieres Clin Haematol 10, 657–689.
3 Aerts JM, Hollak CE, van Breemen M, Maas M,
Groener JE & Boot RG (2005) Identification and use of biomarkers in Gaucher disease and other lysosomal storage diseases. Acta Paediatr Suppl 94, 43–46.
4 Barton NW, Brady RO, Dambrosia JM, Di Bisceglie AM, Doppelt SH, Hill SC, Mankin HJ, Murray GJ, Parker RI, Argoff CE et al. (1991) Replacement therapy for inherited enzyme deficiency – macrophage- targeted glucocerebrosidase for Gaucher’s disease. N Engl J Med 324, 1464–1470.
5 Cox T, Lachmann R, Hollak C, Aerts J, van Weely S, Hrebı´ cek M, Platt F, Butters T, Dwek R, Moyses C et al. (2000) Novel oral treatment of Gaucher’s disease with N-butyldeoxynojirimycin (OGT 918) to decrease substrate biosynthesis. Lancet 355, 1481–1485.
6 Aerts JM, Hollak CE, Boot RG, Groener JE & Maas M (2006) Substrate reduction therapy of glycosphingolipid storage disorders. J Inherit Metab Dis 29, 449–456. 7 Aerts JM & Hollak CE (1997) Plasma and metabolic abnormalities in Gaucher’s disease. Baillieres Clin Haematol 10, 691–709.
The model of Ser102 CHIT1 was based on the crystal struc- ture of native chitotriosidase (Research Collaboratory for Structural Bioinformatics Protein Data Base accession no 1LQ0, resolution 2.20 A˚ ) [34]. The glycine at position 102 was converted into a serine using the program deepview [40]. The native and modified structures were subjected to energy minimalization in gromacs version 3.3.1 with the GROMOS96 force field using the steepest decent method [41]. Preparation of the systems for MD included solvation of the protein structure in a periodic, cubic box, addition of polar and aromatic hydrogen atoms (at a pH of 5.2), addi- tion of Simple Point Charge water molecules [42], removal of water molecules residing in hydrophobic cavities and charge neutralization by exchanging waters with chloride ions. Prior to actual MD, the systems were subjected to another round of energy minimization, followed by 20 ps of MD with position restraints on heavy protein atoms and an unconstrained equilibration run of 1 ns. Both the tempera- ture and pressure in the systems was kept constant, at 300 K and 1 bar, respectively, using the Berendsen thermostat and barostat. Bonded interactions were described using the GROMOS96 force field, van der Waals interactions and short-range electrostatic interactions were treated with a cut-off radius of 1.0 nm and long-range electrostatic inter- actions were treated using the particle mesh Ewald method [43]. Using the LINCS algorithm to constrain bonds [44] allowed for a timestep of 2 fs. Prepared as such, the dynamics of the two systems were sampled during three initiated from different separate MD runs of 10 ns, starting velocities. From the resulting trajectories r.m.s.f. were calculated using the tools included in the gromacs software package.
The data were analysed using the Mann–Whitney U-test. Correlations were tested by the rank correlation test (Spear- man coefficient, q). P values < 0.05 were considered statistically significant.
8 Moller HJ, de Fost M, Aerts H, Hollak C & Moestrup SK (2004) Plasma level of the macrophage-derived soluble CD163 is increased and positively correlates with sever- ity in Gaucher’s disease. Eur J Haematol 72, 135–139. 9 Boot RG, Verhoek M, de Fost M, Hollak CE, Maas M, Bleijlevens B, van Breemen MJ, van Meurs M, Boven LA, Laman JD et al. (2004) Marked elevation of the chemokine CCL18 ⁄ PARC in Gaucher disease: a novel surrogate marker for assessing therapeutic intervention. Blood 103, 33–39.
Statistical analysis
Acknowledgements
10 Hollak CE, van Weely S, van Oers MH & Aerts JM (1994) Marked elevation of plasma chitotriosidase activity. A novel hallmark of Gaucher disease. J Clin Invest 93, 1288–1292.
11 Renkema GH, Boot RG, Strijland A, Donker-Koop- man WE, van den Berg M, Muijsers AO & Aerts JM (1997) Synthesis, sorting, and processing into distinct isoforms of human macrophage chitotriosidase. Eur J Biochem 244, 279–285.
12 Boven LA, van Meurs M, Boot RG, Mehta A,
skilful
Boon L, Aerts JM & Laman JD (2004) Gaucher cells demonstrate a distinct macrophage phenotype and
FEBS Journal 276 (2009) 5678–5688 ª 2009 The Authors Journal compilation ª 2009 FEBS
5686
the The authors wish to thank Hans Vissers from Waters Corporation for his help with the label-free LC-MS method. We gratefully acknowledge SARA Computing and Networking Services for allowing use of the LISA cluster and their technical assistance. We acknowledge our clinical colleagues Maaike Wiersma, Mirjam Langeveld, Mario Maas and Maaike de Fost collection of patient materials and records. for Acknowledged is support by the continuous Netherlands Gaucher patient society. The described
A. P. Bussink et al.
Substrate specificity of G102S chitotriosidase
resemble alternatively activated macrophages. Am J Clin Pathol 122, 359–369.
13 Boot RG, Renkema GH, Strijland A, van Zonneveld AJ & Aerts JM (1995) Cloning of a cDNA encoding chitotriosidase, a human chitinase produced by macro- phages. J Biol Chem 270, 26252–26256.
24 de Fost M, Hollak CE, Groener JE, Aerts JM, Maas M, Poll LW, Wiersma MG, Haussinger D, Brett S, Brill N et al. (2006) Superior effects of high-dose enzyme replacement therapy in type 1 Gaucher disease on bone marrow involvement and chitotriosidase levels: a 2-center retrospective analysis. Blood 108, 830–835. 25 Deegan PB, Moran MT, McFarlane I, Schofield JP,
14 Renkema GH, Boot RG, Muijsers AO, Donker-Koop- man WE & Aerts JM (1995) Purification and charac- terization of human chitotriosidase, a novel member of the chitinase family of proteins. J Biol Chem 270, 2198–2202.
15 Guo Y, He W, Boer AM, Wevers RA, de Bruijn AM, Groener JE, Hollak CE, Aerts JM, Galjaard H & van Diggelen OP (1995) Elevated plasma chitotriosidase activity in various lysosomal storage disorders. J Inherit Metab Dis 18, 717–722.
Boot RG, Aerts JM & Cox TM (2005) Clinical evalua- tion of chemokine and enzymatic biomarkers of Gaucher disease. Blood Cells Mol Dis 35, 259–267. 26 Elstein D, Hollak C, Aerts JM, van Weely S, Maas M, Cox TM, Lachmann RH, Hrebicek M, Platt FM, But- ters TD et al. (2004) Sustained therapeutic effects of oral miglustat (Zavesca, N-butyldeoxynojirimycin, OGT 918) in type I Gaucher disease. J Inherit Metab Dis 27, 757–766.
27 Elstein D, Dweck A, Attias D, Hadas-Halpern I, Zevin S, Altarescu G, Aerts JM, van Weely S & Zimran A (2007) Oral maintenance clinical trial with miglustat for type I Gaucher disease: switch from or combina- tion with intravenous enzyme replacement. Blood 110, 2296–2301.
16 Ries M, Schaefer E, Luhrs T, Mani L, Kuhn J, Vanier MT, Krummenauer F, Gal A, Beck M & Mengel E (2006) Critical assessment of chitotriosidase analysis in the rational laboratory diagnosis of children with Gaucher disease and Niemann–Pick disease type A ⁄ B and C. J Inherit Metab Dis 29, 647–652.
17 Wajner A, Michelen K, Burin MB, Pires RF, Pereira ML, Giugliani R & Coelho JC (2004) Biochemical characterization of chitotriosidase enzyme: comparison between normal individuals and patients with Gaucher and with Niemann–Pick diseases. Clin Biochem 37, 893–897.
28 Aguilera B, Ghauharali-van der Vlugt K, Helmond MT, Out JM, Donker-Koopman WE, Groener JE, Boot RG, Renkema GH, van der Marel GA, van Boom JH et al. (2003) Transglycosidase activity of chi- totriosidase: improved enzymatic assay for the human macrophage chitinase. J Biol Chem 278, 40911–40916.
29 Schoonhoven A, Rudensky B, Elstein D, Zimran A, Hollak CE, Groener JE & Aerts JM (2007) Monitor- ing of Gaucher patients with a novel chitotriosidase assay. Clin Chim Acta 381, 136–139.
30 Boot RG, Renkema GH, Verhoek M, Strijland A,
18 Brinkman J, Wijburg FA, Hollak CE, Groener JE, Verhoek M, Scheij S, Aten J, Boot RG & Aerts JM (2005) Plasma chitotriosidase and CCL18: early bio- chemical surrogate markers in type B Niemann–Pick disease. J Inherit Metab Dis 28, 13–20.
19 Vedder AC, Cox-Brinkman J, Hollak CE, Linthorst
Bliek J, de Meulemeester TM, Mannens MM & Aerts JM (1998) The human chitotriosidase gene. Nature of inherited enzyme deficiency. J Biol Chem 273, 25680– 25685.
30a Gray PW (1997) Chitinase materials and methods.
GE, Groener JE, Helmond MT, Scheij S & Aerts JM (2006) Plasma chitotriosidase in male Fabry patients: a marker for monitoring lipid-laden macrophages and their correction by enzyme replacement therapy. Mol Genet Metab 89, 239–244.
20 Michelakakis H, Dimitriou E & Labadaridis I (2004) The expanding spectrum of disorders with elevated plasma chitotriosidase activity: an update. J Inherit Metab Dis 27, 705–706.
ICOS Corp., Bothell, WA. Patent no. WO9747752. 31 Grace ME, Balwani M, Nazarenko I, Prakash-Cheng A & Desnick RJ (2007) Type 1 Gaucher disease: null and hypomorphic novel chitotriosidase mutations – implications for diagnosis and therapeutic monitoring. Hum Mutat 28, 866–873.
32 Lee P, Waalen J, Crain K, Smargon A & Beutler E
21 Hollak CE, Maas M & Aerts JM (2001) Clinically rele- vant therapeutic endpoints in type I Gaucher disease. J Inherit Metab Dis 24 (Suppl 2), 97–105. 22 Mistry PK & Abrahamov A (1997) A practical
(2007) Human chitotriosidase polymorphisms G354R and A442V associated with reduced enzyme activity. Blood Cells Mol Dis 39, 353–360.
approach to diagnosis and management of Gaucher’s disease. Baillieres Clin Haematol 10, 817–838. 23 Weinreb NJ, Charrow J, Andersson HC, Kaplan P,
33 Vissers JP, Langridge JI & Aerts JM (2007) Analysis and quantification of diagnostic serum markers and protein signatures for Gaucher disease. Mol Cell Prote- omics 6, 755–766.
34 Fusetti F, von Moeller H, Houston D, Rozeboom HJ,
Dijkstra BW, Boot RG, Aerts JM & van Aalten DM (2002) Structure of human chitotriosidase. Implications
Kolodny EH, Mistry P, Pastores G, Rosenbloom BE, Scott CR, Wappner RS et al. (2002) Effectiveness of enzyme replacement therapy in 1028 patients with type 1 Gaucher disease after 2 to 5 years of treatment: a report from the Gaucher Registry. Am J Med 113, 112–119.
FEBS Journal 276 (2009) 5678–5688 ª 2009 The Authors Journal compilation ª 2009 FEBS
5687
A. P. Bussink et al.
Substrate specificity of G102S chitotriosidase
for specific inhibitor design and function of mamma- lian chitinase-like lectins. J Biol Chem 277, 25537– 25544.
39 van Eijk M, van Roomen CP, Renkema GH, Bussink AP, Andrews L, Blommaart EF, Sugar A, Verhoeven AJ, Boot RG & Aerts JM (2005) Characterization of human phagocyte-derived chitotriosidase, a component of innate immunity. Int Immunol 17, 1505–1512. 40 Guex N & Peitsch MC (1997) SWISS-MODEL and
35 Rao FV, Houston DR, Boot RG, Aerts JM, Sakuda S & van Aalten DM (2003) Crystal structures of allos- amidin derivatives in complex with human macrophage chitinase. J Biol Chem 278, 20110–20116.
36 Malaguarnera L, Simpore J, Prodi DA, Angius A,
the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis 18, 2714–2723.
41 van Gunsteren WF & Berendsen HJC (1987)
GROMOS-87 Manual. BIOMOS BV, Groningen, The Netherlands.
Sassu A, Persico I, Barone R & Musumeci S (2003) A 24-bp duplication in exon 10 of human chitotriosidase gene from the sub-Saharan to the Mediterranean area: role of parasitic diseases and environmental conditions. Genes Immun 4, 570–574.
37 Rodrigues MR, Sa Miranda MC & Amaral O (2004)
Allelic frequency determination of the 24-bp chitotrios- idase duplication in the Portuguese population by real- time PCR. Blood Cells Mol Dis 33, 362–364.
42 Berendsen HJ, Postma JP, van Gunsteren WF & Hermans J (1981) Interaction models for water in relation to protein hydration. In Intermolecular Forces (Pullman B ed), pp. 331–342. D. Reidel, Dordrecht. 43 Lindahl E, Hess B & van der Spoel D (2001) GRO- MACS 3.0: a package for molecular simulation and trajectory analysis. J Mol Model 7, 306–317.
38 Ghauharali-van der Vlugt K, Bussink AP, Groener JE, Boot RG & Aerts JM (2009) Detection of chitinase activity by 2-aminobenzoic acid labeling of chito-oligo- saccharides. Anal Biochem 384, 191–193.
44 Hess B, Bekker B, Berendsen HJ & Fraaije JG (1997) LINCS: a linear constraints solver for molecular simu- lations. J Comput Chem 18, 1463–1472.
FEBS Journal 276 (2009) 5678–5688 ª 2009 The Authors Journal compilation ª 2009 FEBS
5688
