JOURNAL OF MEDICAL CASE REPORTS

Ratuapli et al. Journal of Medical Case Reports 2011, 5:5 http://www.jmedicalcasereports.com/content/5/1/5

C A S E R E P O R T

Open Access

Epstein Barr Virus-positive large T-cell lymphoma presenting as acute appendicitis 17 years after cadaveric renal transplant: a case report Shiva K Ratuapli1*, Shishir Murarka1, Karen A Miller2, James C Ferraro1, Haider Zafar1

Abstract

Introduction: The majority of post-transplant lymphoproliferative disorders in renal transplant patients are of the B-cell phenotype, while the T-cell phenotype is rare. We report a case of Epstein Barr Virus-positive, T-cell lymphoma in a renal transplant patient, presenting unusually as acute appendicitis.

Case presentation: A 45-year-old Hispanic male renal transplant patient presented with right-side abdominal pain 17 years after transplant. The laboratory studies were unremarkable. Laparoscopic exploration showed an inflamed appendix so a laparoscopic appendectomy was performed. Pathology of the appendix showed large cells positive for CD3, CD56 and Epstein Barr Virus-encoded RNA staining, and negative for CD20 and CD30. The tissue tested positive for T-cell receptor gene rearrangement by polymerase chain reaction analysis. Treatment management involved reduction of immunosuppression and initiation of chemotherapy with cisplatin, etoposide, gemcitabine, and solumedrol followed by cyclophosphamide, hydroxydaunorubicin, vincristine and prednisone). He recovered and the allo-grafted kidney is fully functional. Conclusion: We report a rare case of post-renal transplant large T-cell lymphoma, with an unusual presentation of acute appendicitis and Epstein Barr Virus-positivity, which responded well to chemotherapy.

Virus (EBV)-positive T-cell lymphoma in a patient, who underwent cadaveric renal transplant 17 years ago and was on chronic multi-drug immunosuppression. Our patient presented unusually with abdominal pain and acute appendicitis.

Introduction Solid organ transplantation has been increasingly per- formed in recent years with the use of highly potent immunosuppressive agents to avoid rejection by the host. Post-transplant lymphoproliferative disorders (PTLD) are well known malignancies found in trans- plant patients, with an incidence reportedly 28 to 49 times greater than in the general population [1]. PTLD in renal transplant patients is reported to be higher in the paediatric population (10.1%) than the adult popula- tion (1.2%) [2]. PTLD in renal transplant patients was first described as a complication with azathioprine-based therapy [3], but was later described after therapy with multiple more novel immunosuppressive agents.

While the majority of PTLD in renal transplant patients are of the B-cell phenotype, a few exhibit the T-cell phenotype [4]. We report a case of Epstein Barr

Case presentation A 45-year-old Hispanic male who underwent cadaveric renal transplant in the right lower quadrant 17 years earlier, presented to the hospital with a three-month history of generalized abdominal pain with localization to the right side for two weeks. He was on chronic immunosuppression with tacrolimus, azathioprine, siro- limus and prednisone. The pain was more pronounced in the right upper quadrant, and the ultrasound imaging of the abdomen was suggestive of cholecystitis. Labora- tory studies did not reveal any abnormalities. He could not confirm if he had had any problems or surgeries on his gall bladder. Hence, he underwent laparoscopic exploration of the gall bladder fossa. During surgery, adhesions of the omentum were found in the gall

* Correspondence: shiva.ratuapli@bannerhealth.com 1Department of Medicine, Banner Estrella Medical Center, 9201 W. Thomas Road, Phoenix, AZ 85037, USA Full list of author information is available at the end of the article

© 2011 Ratuapli et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

with the 3.3 cm liver mass. Non-specific uptake in the stomach was also observed.

bladder fossa in the absence of the gall bladder, and an inflamed appendix was found elevated due to the trans- planted kidney in the right lower quadrant. Laparoscopic appendectomy was performed and the tissue underwent pathological examination. He was discharged after an uneventful post-operative course.

The patient was re-admitted to the hospital 10 days later, with increasing abdominal pain, symptoms of gas- tric outlet obstruction, weight loss, headaches and fever. A lumbar puncture was negative for infection or lym- phoma. Cranial imaging with a computed tomography (CT) scan was also negative. An esophagogastroduode- noscopy (EGD) was performed revealing multiple ulcer- ated nodular masses in the stomach and duodenum (Figures 6 and 7). A stomach biopsy gave similar results as the appendix with large anaplastic cells with irregular nuclei. Immunostaining of the gastric specimen con- firmed T-cell lymphoma as well as positive EBER staining.

Pathology of his appendix by immunostaining revealed anaplastic cells strongly positive for CD3, CD56 together with strong focal EBV-encoded RNA (EBER) staining (Figures 1, 2, 3 and 4). The malignant cells were nega- tive for CD20, CD30, CD45, CD5, Alk-1 and TCK. The tissue was found to be positive for the T-cell receptor (TCR) by gamma gene rearrangement studies by PCR analysis (Figure 5). Immunoglobulin heavy chain rear- rangement (IgH) by PCR analysis did not detect a clonal B-cell population, thereby confirming T-cell lymphoma. A bone marrow examination revealed no involvement with negative flow cytometry and showed normal male karyotype (46, XY). A staging positron emission tomo- graphy (PET) scan showed increased radiotracer uptake in the right cervical and left groin lymph nodes along

Initial treatment management involved reducing the dose of the patient’s immunosuppressive agents and starting chemotherapy. Administration of azathioprine, prednisone and tacrolimus was stopped and low dose sirolimus at 1 mg was given daily. The first cycle of chemotherapy (PEGS) included cisplatin 25 mg/m2,

Page 2 of 8 Ratuapli et al. Journal of Medical Case Reports 2011, 5:5 http://www.jmedicalcasereports.com/content/5/1/5

Figure 1 Appendix biopsy showing large, pleomorphic lymphocytes with irregular nuclear contours and large nucleoli. (400 X).

Page 3 of 8 Ratuapli et al. Journal of Medical Case Reports 2011, 5:5 http://www.jmedicalcasereports.com/content/5/1/5

Figure 2 Positive staining of lymphoid infiltrate for CD3 (400 X).

Figure 3 Gastric biopsy showing atypical lymphoid infiltrate (200 X).

Page 4 of 8 Ratuapli et al. Journal of Medical Case Reports 2011, 5:5 http://www.jmedicalcasereports.com/content/5/1/5

Figure 4 Appendiceal infiltrate showing scattered Epstein Barr Virus-positive cells (100 X).

Discussion Our case is interesting due to the latency of PTLD development, the lack of hematological abnormalities and the EBV-positivity, even though the lymphoma was of T-cell origin. The diagnosis became apparent when an appendectomy was performed for abdominal pain. The cytopathology of our patient shows all the typical features of a peripheral T-cell variant of PTLD, where the T-cell lineage of the lymphoma was confirmed by TCR gene rearrangement studies.

etoposide 40 mg/m2 and solumedrol 250 mg adminis- tered on days one, two and three, and gemcitabine (Gemzar) 1000 mg/m2 on day one (ongoing Phase II trial SWOG 0350). Our patient had a positive and rapid clinical response to this regimen. Thus, the chemother- apy was changed to a standard CHOP regimen (cyclo- phosphamide, doxorubicin [Adriamycin], vincristine, prednisone). His gastric outlet obstruction was sup- ported with total parenteral nutrition (TPN) for a few weeks, after which the patient was able to eat well. A repeat PET scan after the second cycle of CHOP showed a significant response.

While presenting symptoms in the majority of patients are non-specific such as fever and weight loss, approxi- mately 15% of cases present as an emergency with intest- inal perforation [5]. Similar to other reports on T-cell type PTLD, which generally occurred more than five years after transplant, this case occurred 17 years after the renal transplant. The high levels of immunosuppres- sion were due to two episodes of graft rejection in the preceding four years.

The main complications during the therapy were pan- cytopenia, febrile neutropenia and pneumonia. These were managed successfully. He recovered well and is presently receiving treatment as an outpatient. His allo- grafted kidney is also fully functional. Restaging is planned after a total of six cycles of CHOP with a PET scan and EGD.

Page 5 of 8 Ratuapli et al. Journal of Medical Case Reports 2011, 5:5 http://www.jmedicalcasereports.com/content/5/1/5

unspecified type. While the incidence of T-cell PTLD in renal transplant patients is approximately 15%, a recent study of 21 cases of post-transplant hepatosplenic T-cell lymphoma by Tey et al. [7] reported 19 patients who underwent prior renal transplant. This and several other reports [5,7,8] appear to show increased incidence, which might be due to heightened awareness along with use of increasingly potent immunosuppressants.

The etiopathogenesis of T-cell PTLD is not entirely known, although it may be similar to non-Hodgkins lymphoma (NHL) seen in the general population. While

PTLD can be categorized into three distinct groups based on the World Health Organization classification of lymphoid tissue neoplasms [6] (Table 1). The first group has diffuse B-cell hyperplasia, which is relatively benign and responds well to a reduction in immunosup- pression. The second group consists of polymorphic PTLD, which is the most common group in both the adult and pediatric populations. The third group con- sists of high-grade invasive lymphomas of either T- or B-cell monoclonality. Monomorphic T-cell PTLD is further subdivided into large cell, anaplastic or an

Figure 5 T cell receptor gene rearrangement by PCR analysis showing monoclonal spike.

Page 6 of 8 Ratuapli et al. Journal of Medical Case Reports 2011, 5:5 http://www.jmedicalcasereports.com/content/5/1/5

a putative role of EBV has been suggested in 89% of cases of B-cell PTLD [9], no direct role for this lympho- tropic virus has been confirmed in T-cell PTLD. EBV infects and immortalizes B cells causing unchecked pro- liferation of EBV-infected cells, as the critical T-cell control of B-cells is lacking in immunosuppressed patients [10]. Human T-cell Lymphotropic Virus 1 (HTLV1) has been reported to cause post renal trans- plant T-cell lymphomas in Japan [11], due to a higher prevalence of the virus among hemodialysis patients. Our case was unusual in that the lymphoma tested posi- tive for EBV, even though it was of T-cell origin, which is only seen in a small minority of patients [12].

in 25% to 63% [13] of adults. Early polyclonal PTLD responds well to a reduction in immunosuppression, whereas monoclonal PTLD generally does not respond to the reduction and has a high mortality rate of 50% to 90% [14]. Early use of anthracycline-based chemotherapy results in long term disease free survival rates of 50% to 60% in monoclonal B-cell lymphomas [15], whereas T-cell PTLD responds very poorly. There are no stan- dardized chemotherapy regimens for PTLD in general and for the T-cell phenotype in particular. Multiple treatment regimens such as CHOP, VAPEC-B (Adria- mycin, etoposide, cyclophosphamide, methotrexate, bleomycin and vincristine), anti-IL-6 mAb, bexarotene and antiviral agents have been used with varied results. Other salvage regimens for high-grade lymphomas have also been suggested in the literature [14,15].

The initial step in treating PTLD is reduction in immunosuppression, and the response is usually seen in three to four weeks, resulting in long term remission

Figure 6 EGD showing large ulcerated gastric nodule together with large nodules in the duodenum.

Page 7 of 8 Ratuapli et al. Journal of Medical Case Reports 2011, 5:5 http://www.jmedicalcasereports.com/content/5/1/5

large multi-centre studies are required to understand the pathogenesis and develop better treatment regimens.

Figure 7 EGD showing multiple large gastric nodules with central ulceration.

Consent Written informed consent was obtained from the patient for publication of this case report and accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.

Conclusion In summary we report the case of a patient with post-renal transplant large T-cell lymphoma, with an unusual presen- tation of acute appendicitis and EBV positivity. We report successful treatment with chemotherapy and stress the need for heightened awareness of this malignancy in patients with prolonged immunosuppression. Monoclonal T-cell PTLD remains a high mortality disease, and further

Table 1 The World Health Organization classification of PTLD

Early Lesions Polymorphic PTLD Monomorphic PTLD

Reactive Plasmacytic Hyperplasia Polyclonal Monoclonal Other Types 1. Hodgkin’s disease- like 2. Plasmacytoma-like

B-Cell Lymphomas (cid:129) Diffuse large B cell lymphoma (cid:129) Burkitt/Burkitt-like lymphoma (cid:129) Plasma cell myeloma T-Cell Lymphomas (cid:129) Peripheral T cell lymphoma (cid:129) Large CellAnaplastic (cid:129) Unspecified (cid:129) Raretypes (gammadelta, Hepatosplenic, T/NKcell)

Author details 1Department of Medicine, Banner Estrella Medical Center, 9201 W. Thomas Road, Phoenix, AZ 85037, USA. 2Department of Pathology, Banner Estrella Medical Center, 9201 W. Thomas Road, Phoenix, AZ 85037, USA.

Authors’ contributions SKR and HZ participated in the conception and literature search. KAM provided and reviewed the pathology slides. SKR, SM, JF and HZ helped to draft the manuscript. All authors read and approved the final manuscript.

Competing interests The authors declare that they have no competing interests.

Received: 20 February 2010 Accepted: 12 January 2011 Published: 12 January 2011

References 1.

2.

3.

4.

5.

6.

7.

8.

9.

Boubenider S, Hiesse C, Goupy C, Kriaa F, Marchand S, Charpentier B: Incidence and consequences of post-transplantation lymphoproliferative disorders. J Nephrol 1997, 10:136-145. Shapiro R, Nalesnik M, McCauley J, Fedorek S, Jordan ML, Scantlebury VP, Jain A, Vivas C, Ellis D, Lombardozzi-Lane S, Randhawa P, Johnston J, Hakala TR, Simmons RL, Fung JJ, Starzl TE: Posttransplant lymphoproliferative disorders in adult and pediatric renal transplant patients receiving tacrolimus-based immunosuppression. Transplantation 1999, 68:1851-1854. Penn I, Hammond W, Brettschneider L, Starzl TE: Malignant lymphomas in transplantation patients. Transplant Proc 1969, 1:106-112. Leblond V, Sutton L, Dorent R, Davi F, Bitker MO, Gabarre J, Charlotte F, Ghoussoub JJ, Fourcade C, Fischer A, et al: Lymphoproliferative disorders after organ transplantation: a report of 24 cases observed in a single center. J Clin Oncol 1995, 13:961-968. Taylor AL, Marcus R, Bradley JA: Post-transplant lymphoproliferative disorders (PTLD) after solid organ transplantation. Crit Rev Oncol Hematol 2005, 56:155-167. Harris NL, Jaffe ES, Diebold J, Flandrin G, Muller-Hermelink HK, Vardiman J, Lister TA, Bloomfield CD: The World Health Organization classification of hematological malignancies report of the Clinical Advisory Committee Meeting, Airlie House, Virginia, November 1997. Mod Pathol 2000, 13:193-207. Tey SK, Marlton PV, Hawley CM, Norris D, Gill DS: Post-transplant hepatosplenic T-cell lymphoma successfully treated with HyperCVAD regimen. Am J Hematol 2008, 83:330-333. Balachandran I, Walker JW Jr, Broman J: Fine needle aspiration cytology of ALK 1(-), CD 30(+) anaplastic large cell lymphoma post renal transplantation: A case report and literature review. Diagn Cytopathol 2010, 38:213-216. Pasquale MA, Weppler D, Smith J, Icardi M, Amador A, Gonzalez M, Kato T, Tzakis A, Ruiz P: Burkitt’s lymphoma variant of post-transplant lymphoproliferative disease (PTLD). Pathol Oncol Res 2002, 8:105-108. 10. Cohen JI: Epstein-Barr virus infection. N Engl J Med 2000, 343:481-492. 11. Hoshida Y, Li T, Dong Z, Tomita Y, Yamauchi A, Hanai J, Aozasa K:

Lymphoproliferative disorders in renal transplant patients in Japan. Int J Cancer 2001, 91:869-875.

12. Magro CM, Weinerman DJ, Porcu PL, Morrison CD: Post-transplant EBV- negative anaplastic large-cell lymphoma with dual rearrangement: a propos of two cases and review of the literature. J Cutan Pathol 2007, 34:1-8.

13. Gottschalk S, Rooney CM, Heslop HE: Post-transplant lymphoproliferative

disorders. Annu Rev Med 2005, 56:29-44.

14. Orjuela M, Gross TF, Cheung YK, Alobeid B, Morris E, Cairo MS: A pilot

Page 8 of 8 Ratuapli et al. Journal of Medical Case Reports 2011, 5:5 http://www.jmedicalcasereports.com/content/5/1/5

Submit your next manuscript to BioMed Central and take full advantage of:

• Convenient online submission

15.

• Thorough peer review

• No space constraints or color figure charges

study of chemoimmunotherapy (cyclophosphamide, prednisone, and rituximab) in patients with post-transplant lymphoproliferative disorder following solid organ transplantation. Clin Cancer Res 2003, 9:3945S-3952S. Taylor AL, Bowles KM, Callaghan CJ, Wimperis JZ, Grant JW, Marcus RE, Bradley JA: Anthracycline-based chemotherapy as first-line treatment in adults with malignant posttransplant lymphoproliferative disorder after solid organ transplantation. Transplantation 2006, 82:375-381.

• Immediate publication on acceptance

• Inclusion in PubMed, CAS, Scopus and Google Scholar

• Research which is freely available for redistribution

doi:10.1186/1752-1947-5-5 Cite this article as: Ratuapli et al.: Epstein Barr Virus-positive large T-cell lymphoma presenting as acute appendicitis 17 years after cadaveric renal transplant: a case report. Journal of Medical Case Reports 2011 5:5.

Submit your manuscript at www.biomedcentral.com/submit