MINISTRY OF EDUCATION AND
TRAINING
MINISTRY OF AGRICULTURE AND RURAL
DEVELOPMENT
VIETNAM ACADEMY OF AGRICULTURAL SCIENCES
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TRAN THI BINH NGUYEN
EVALUATING GENETIC RESOURCES AND ANALYZING MOLECULAR
MARKERS RELATED TO EGG YIELD TRAITS IN LIEN MINH
CHICKEN BREED
Specialization: Biotechnology
Code number: 9420201
SUMMARY OF AGRICULTURAL DOCTORAL THESIS
Hanoi, 2019
The dissertation has been completed at: Vietnam Academy of Agricultural Sciences
Supervisors:
1. Dr. Nguyen Thi Dieu Thuy
2. Dr. Nguyen Huu Duc
Reviewer 1:
Reviewer 2:
Reviewer 3:
The dissertation will be defended at the Dissertation committee at the
National level Vietnam Academy of Agricultural Sciences, on Date
month year
The full text of the dissertation can be found in the following libraries:
- The National Library of Vietnam;
- The Library of Vietnam Academy of Agricultural Sciences.
DANH MỤC CÔNG TRÌNH CÔNG BỐ LIÊN QUAN ĐẾN LUẬN ÁN
1. Tran Thi Binh Nguyen, Nguyen Huu Duc, Do Vo Anh Khoa, Nguyen Thi
Dieu Thuy (2018), “Effect of nucleotide polymorphism of candidate genes on
egg production traits in native Lien Minh chicken”, Livestock Research for
Rural Development 30(6).
2. Tran Thi Binh Nguyen, Nguyen Huu Duc, Vu Duc Quy, Pham Thu Giang,
Nguyen Manh Linh, Dinh Thi Ngoc Thuy, Nguyen Thi Dieu Thuy (2018)
“Polymorphism in candidate genes of Lien Minh chicken”, Vietnam Journal of
Agricultural Sciences 1(2), tr. 174-182.
3. Tran Thi Binh Nguyen, Nguyen Hung Cuong, Vu Thi Tien, Ta Thi Loan,
Do Vo Anh Khoa and Nguyen Thi Dieu Thuy (2018).,“Association of
neuropeptide gene polymorphism with egg production trait in Lien Minh
chicken”, National Biotechnology conference 2018, Hanoi 26/10/2018, pp.
1828-1833.
4. Tran Thi Binh Nguyen, Nguyen Huu Duc and Nguyen Thi Dieu Thuy
(2018), ,“Association of prolactin gene polymorphism with egg production
trait in Lien Minh chicken”, Vietnam Journal of Biotechnology, 16(2), pp. 1-8.
5. Tran Thi Binh Nguyen, Do Thi Lieu, Nguyen Huu Duc, Hoang Thi Yen, Vu
Cong Quy, Nguyen Hung Cuong and Nguyen Thi Dieu Thuy (2016),
,“Reproductive characteristics and preliminary analysis on polymorphism of
candidate genes concerning with egg production traits of Lien Minh chicken
breed”, Journal of Animal Husbandry Sciences and Technics, 212, pp. 2-8
1
INTRODUCTION
1. The necessity of research
Lien Minh chicken is an indigenous breed with several favorable properties, such
as good meat quality and associated with the economic development of the people in
the Lien Minh village, Cat Hai, Hai Phong. Such breed comes in beautiful physical
appearance with its nice feather color, yellow skin and produces high-quality meat
with thinfat under the skin. The source of the Lien Minh chicken gene was assessed at
the level of dangerous threat (FAO, 2007), and has been listed on the conservation list
since 2008. The conservation and efficient use of Lien Minh chicken were developed
in 2013. Research results from more than 30 households studied in Tran Chau
commune, Cat Hai district, Hai Phong city the grazing revealed that, aAge of first egg
laying in Lien Minh chickens was quite late (197.5 days of age) and low egg yield 75.6
eggs/chicken/year (Doan BH et al, 2016). It is therefore essential to increase Lien
Minh chicken egg production in order to conserve, exploit, and develop such poultry
breeds.
Chicken egg production can be affected by a number of factors such as genetics,
nutrition, light, and veterinary care regimes. Among these factors, Genetic
polymorphism is currently studied in order to improve economic characteristics of
indigenous chickens. The polymorphisms of PRL (Prolactin), VIP (Vasoactive
Intestinal Peptide), VIPR1 (Vasoactive Intestinal Peptide Receptor 1), NPY
(Neuropeptide Y), GH (Growth hormone) and GHR (Growth hormone receptor) have
been defined to have significant association with fertility in some in some indigenous
chicken breeds around the world. Therefore, genetic polymorphism can be used as
DNA markers to improve egg production in indigenous chickens.
Additionally, little research has been carried out on the evaluation of the genetic
diversity based on the nucleotide sequence of D-loop mitochondrial genes in Lien
Minh chickens. The results of these studíe can be used to provide information on
genetic diversity, to support for registering the copyright of the Lien Minh chicken
breed. Furthermore, this work is a solid foundation for building the panorama of Lien
Minh chicken’s genetic diversity levels.
Therefore, "Evaluating genetic resources and analyzing molecular markers
related to egg yield traits in Lien chicken breed” was implemented to provide genetic
information of the D-loop region of mitochondrial genes and candidate genes
improving egg production as well as support conservation, exploitation and
development of Lien Minh chicken.
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2. Research objectives
- Evaluate genetic diversity of Lien Minh chickens based on sequences of
mitochondrial DNA D-loop region
- Determine the genetic relationship and the origin of Lien Minh chickens.
- Identif genetic relationships between candidate genes and egg yield traits in
Lien Minh chickens.
3. Scientific and practical significance
* Scientific significance
- Provides the initial information about the sequence of mitochondrial D-loop
region in Lien Minh chickens. The first scientific publication to study the frequency
of allele / genotype occurrence at the polymorphisms of PRL, VIP, VIPR1, NPY, GH,
and GHR in Lien Minh chickens;
- results on candidate genetic polymorphism with egg yield traits are the
scientific database for the study of the application of molecular markers to support the
selection of Lien Minh chickens that have high egg yield. Provide information
molecular markers of indigenous chickens Lien Minh for breeders, reference for
researchers, students.
* Practical significance:
Provide scientific information to promote and develop the market for indigenous
chickens Lien Minh products. Provide scientific information to promote and develop
the market for indigenous chickens Lien Minh products. Provide information for the
Hai Phong Center for Science and Technology Application, companies and livestock
farms to select the line Lien Minh chickens with high egg production.
4. Object and scope of the study
- The thesis applied bioinformatics tools to assessing the genetic resource of Lien
Minh chickens: Analyzing the nucleotide sequence of mtDNA D-loop region of Lien
Minh chicken to assess the diversity of genetic resources.
- Determine genotypic and allelic frequency of at PRL, VIP, VIPR1, NPY, GH,
and GHR genes in Lien Minh chickens.
- Assess the relationship between polymorphisms of PRL, VIP, VIPR1, NPY, GH,
and GHR genes and egg production traits.
5. Subject of research and scope of research
Subjects of research: Lien Minh indigenous chickens in Tran Chau commune,
Cat Hai district, Hai Phong city.
Scope of research: The Thesis applied biotechnology and bioinformatics tools
to analyze the genetic diversity of Lien Minh chicken, and to support conservation,